Date published: 2026-5-17

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m-IgG1 BP-CFL 594: sc-533663

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Datasheets
  • mouse IgG1 binding protein (m-IgG1 BP) conjugated to CruzFluor™ 594 (CFL 594)
  • supplied at 100 µg in 0.5 ml volume
  • CruzFluor™ 594 (CFL 594) is a red fluorescent dye that is an excellent substitute for Alexa Fluor® 594, offering comparable photostability and the ability to resist protein quenching
  • Excitation of 587 nm, Emission of 603 nm
  • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
  • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
  • Suitable for binding to most, but not all, mouse IgG1 immunoglobulins; not suitable for use with mouse monoclonal IgG2a, IgG2b, IgG3, IgM, IgA or IgE antibodies. May slightly cross react with mouse IgG2b and goat IgG antibodies
  • Not cross-reactive with human, rat or rabbit IgG antibodies
  • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
  • Mouse IgG binding proteins are recommended for some, but not all of our monoclonal antibodies. Product descriptions on our monoclonal antibody product pages will state if the Mouse IgG binding protein is the preferred detection reagent for that product.
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    Mouse IgG1 binding protein (IgG1 BP) conjugated to CruzFluor™ 594 (CFL 594) is a strongly recommended alternative to conventional goat/rabbit anti-mouse IgG secondary antibodies for RGB Western Blotting (WB), immunofluorescence (IF) and flow cytometry (FCM) signal enhancement. CruzFluor™ 594 (CFL 594) is a red fluorescent dye that is an excellent substitute for AlexFluor® 594, offering comparable photostability and the ability to resist protein quenching. Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems. Mouse IgG1 binding protein is a highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies. Mouse IgG1 binding protein (m-IgG1 BP) is suitable for binding to most, but not all, mouse IgG1 immunoglobulins, comprising approximately 55% of SCBT's mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgG2a, IgG2b, IgG3, IgM, IgA and IgE antibodies. It may slightly cross react with mouse IgG2b and goat IgG antibodies. Not cross reactive with human, rat and rabbit IgG antibodies.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    m-IgG1 BP-CFL 594

    sc-533663
    100 µg/0.5 ml
    $93.00

    it said "May slightly cross react with mouse IgG2b and goat IgG antibodies". So to what extent it cross react?

    Asked by: wwww
    Thank you for your question. We do not provide specific cross-reactivity ratios .
    Answered by: BlakeJ
    Date published: 2024-07-10
    • y_2026, m_5, d_16, h_14CST
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    Rated 5 out of 5 by from Great for multiple applications[Employee of scbt] Great detection reagent for flow cytometry, immunofluorescence, and fluorescent western blot. For use with primary mouse monoclonal antibodies that bind to mouse IgG1 Binding Protein. -SCBT QC
    Date published: 2023-09-14
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    m-IgG 1 BP-CFL 594 is rated 5.0 out of 5 by 1.
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