Met抗体 (B-2) 推荐用于 mouse 和 rat 来源的Met WB, IP, IF, IHC(P) 和 ELISA检测

Met 抗体 (B-2): sc-8057

Met抗体 (B-2) is rated 4.5 out of 5 by 13.
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说明书

    • 抗-Met 抗体 B-2 是小鼠单克隆 IgG1 (kappa轻链),Met抗体,规格为200 µg/ml
    • 特异性抗原位于mouse物种的c-Met p140的C-terminus的氨基酸1359-1379之间
    • 抗-Met 抗体 (B-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse 和rat 来源的 Met
    • 抗-Met 抗体 (B-2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 封闭肽,sc-8057 P
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Met (B-2): sc-8057免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

What is the recommended secondary for use with this Met Antibody (B-2) mouse monoclonal antibody?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 8
Date published: 2017-01-09
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Rated 5 out of 5 by from So far so good Works very well for IF on mouse kidney cryosections.
Date published: 2019-04-19
Rated 2 out of 5 by from Did not work in mouse liver lysate Did not work in mouse liver lysate, I tried it with 1:500 dilutions
Date published: 2018-04-12
Rated 2 out of 5 by from Not specific The antibody is very strong and clean but it detects a band near 30-kDa in MCF-7 cells.
Date published: 2017-09-11
Rated 5 out of 5 by from specific bands The specific bands were detected by Western blot and 1:500 in mouse kidney tissues.
Date published: 2017-05-24
Rated 5 out of 5 by from WB 结果很不错 用鼠的内皮细胞提取蛋白,条带很特异。背景很干净。 用去石蜡切片免疫荧光效果还不清楚。过段时间再评论。
Date published: 2017-03-09
Rated 5 out of 5 by from Superb !! Outstanding western data; strong signal with low background; also great IFC staining !!
Date published: 2017-01-27
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-12
Rated 5 out of 5 by from Positive detection of the Met protein by Positive detection of the Met protein by WB using LADMAC whole cell lysate. -SCBT QC
Date published: 2015-04-07
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