Date published: 2025-9-8

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Mesothelin 抗体 (K1): sc-33672

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说明书
  • Mesothelin 抗体 K1 是小鼠单克隆 IgG1 κ,Mesothelin抗体, 在16篇文献中引用,规格为200 µg/ml
  • 针对与OVCAR-3卵巢肿瘤细胞系融合的小鼠脾淋巴细胞
  • Mesothelin 抗体 (K1) 推荐用于 WB, IP, IF 和 FCM,检测mouse, rat 和human 来源的 Mesothelin
  • 抗Mesothelin抗体(K1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mesothelin (K1): sc-33672免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Mesothelin Antibody (K1) for WB applications. 的首选辅助检测试剂,这些试剂现与Mesothelin Antibody (K1) 打包提供(请参阅下面的订购信息)。

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関連項目

间皮素抗体(K1)是一种小鼠单克隆IgG1κ轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和流式细胞术(FCM)检测小鼠、大鼠和人源的间皮素蛋白。抗间皮素抗体(K1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。间皮素是一种以糖基磷脂酰肌醇为连接的细胞表面蛋白,主要表达于体腔的间皮衬里,在包括卵巢癌和间皮瘤在内的多种恶性肿瘤中显著过度表达。间皮素在细胞粘附和信号传导中发挥着关键作用,对于维持组织完整性和促进细胞交流至关重要。肿瘤中过量的间皮素使这种蛋白质成为癌症免疫治疗的重要靶标,因为间皮素可以作为诊断的生物标记和潜在的治疗靶标。在成年小鼠组织中,间皮素转录物存在于肺、心脏、脾脏、肝脏、肾脏和睾丸中,这凸显了间皮素在正常生理和疾病状态下的多种生物学作用和潜在意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mesothelin 抗体 (K1) 参考文献:

  1. 小鼠的正常发育或繁殖不需要中皮素。  |  Bera, TK. and Pastan, I. 2000. Mol Cell Biol. 20: 2902-6. PMID: 10733593
  2. 对常见人类癌症中间皮素表达的大规模分子和组织芯片分析。  |  Frierson, HF., et al. 2003. Hum Pathol. 34: 605-9. PMID: 12827615
  3. 卵巢癌抗原 CA125/MUC16 与间皮素的结合介导了细胞粘附。  |  Rump, A., et al. 2004. J Biol Chem. 279: 9190-8. PMID: 14676194
  4. 间皮素特异性 CAR T 细胞靶向卵巢癌。  |  Schoutrop, E., et al. 2021. Cancer Res. 81: 3022-3035. PMID: 33795251
  5. IL-7和CCL19分泌的CAR-T细胞疗法可用于治疗糖蛋白-3或间皮素阳性的肿瘤。  |  Pang, N., et al. 2021. J Hematol Oncol. 14: 118. PMID: 34325726
  6. PD-1和TCR干扰CAR-T细胞治疗间皮瘤阳性实体瘤的I期研究。  |  Wang, Z., et al. 2021. Cell Mol Immunol. 18: 2188-2198. PMID: 34381179
  7. 开发用于治疗胃癌的间皮素特异性 CAR NK-92 细胞。  |  Cao, B., et al. 2021. Int J Biol Sci. 17: 3850-3861. PMID: 34671203
  8. 两例高活性间皮素定向 CAR T 细胞引起的严重肺毒性。  |  Haas, AR., et al. 2023. Mol Ther. 31: 2309-2325. PMID: 37312454
  9. 胰腺癌细胞的间皮素分泌会吸引巨噬细胞并促进转移。  |  Luckett, T., et al. 2024. Cancer Res. 84: 527-544. PMID: 38356443
  10. 间皮素的分子克隆,间皮素是一种存在于间皮细胞, 间皮瘤和卵巢癌上的分化抗原。  |  Chang, K. and Pastan, I. 1996. Proc Natl Acad Sci U S A. 93: 136-40. PMID: 8552591
  11. 从噬菌体展示文库中分离抗间皮素抗体。  |  Chowdhury, PS., et al. 1997. Mol Immunol. 34: 9-20. PMID: 9182872
  12. 111铟标记的单克隆抗体K1:在携带表达间皮素的人类癌症异种移植物的裸鼠中进行的生物分布研究。  |  Hassan, R., et al. 1999. Int J Cancer. 80: 559-63. PMID: 9935157

订购信息

产品名称产品编号规格价格数量收藏夹

Mesothelin 抗体 (K1)

sc-33672
200 µg/ml
$316.00

Mesothelin (K1): m-IgG Fc BP-HRP 套装

sc-526701
200 µg Ab; 10 µg BP
$354.00

Mesothelin (K1): m-IgG1 BP-HRP 套装

sc-532074
200 µg Ab; 20 µg BP
$354.00

Mesothelin 抗体 (K1) AC

sc-33672 AC
500 µg/ml, 25% agarose
$416.00

Mesothelin 抗体 (K1) HRP

sc-33672 HRP
200 µg/ml
$316.00

Mesothelin 抗体 (K1) FITC

sc-33672 FITC
200 µg/ml
$330.00

Mesothelin 抗体 (K1) PE

sc-33672 PE
200 µg/ml
$343.00

Mesothelin 抗体 (K1) Alexa Fluor® 488

sc-33672 AF488
200 µg/ml
$357.00

Mesothelin 抗体 (K1) Alexa Fluor® 546

sc-33672 AF546
200 µg/ml
$357.00

Mesothelin 抗体 (K1) Alexa Fluor® 594

sc-33672 AF594
200 µg/ml
$357.00

Mesothelin 抗体 (K1) Alexa Fluor® 647

sc-33672 AF647
200 µg/ml
$357.00

Mesothelin 抗体 (K1) Alexa Fluor® 680

sc-33672 AF680
200 µg/ml
$357.00

Mesothelin 抗体 (K1) Alexa Fluor® 790

sc-33672 AF790
200 µg/ml
$357.00

What is Molecular weight of cat. sc-33672 antibody? Is it 69KDa? Also, I would like to ask what is K1s' epitope sequence. (I wonder where the antibody binding. CAT. sc-33672 antibodys' MSLN clone binding site)

Asked by: dasts111
Thank you for your question. Molecular Weight of Mesothelin precursor: 69 kDa. Molecular Weight of mature Mesothelin: 40 kDa. sc-33672 raised against spleen lymphocytes from mice fused with OVCAR-3 ovarian tumor cell line. We do not provide specific epitope information.
Answered by: Ling Chen
Date published: 2023-05-05

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-33672 PE), fluorescein (sc-33672 FITC), Alexa Fluor® 488 (sc-33672 AF488) or Alexa Fluor® 647 (sc-33672 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Service 11
Date published: 2021-12-29
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Rated 5 out of 5 by from 功能非常出色使用了这个产品,目前实验结果稳定,后续会多加考虑。目前在方法开发上面,得到一个很大得帮忙,后续稳定后会增加一些新产品得测试。
Date published: 2025-07-31
Rated 5 out of 5 by from MesothelinDetection of Mesothelin expression in corneal endothelial cells with Western Blotting
Date published: 2017-05-19
Rated 4 out of 5 by from Mesothelin Antibody (K1) Alexa Fluor ® 647Mesothelin Antibody (K1) Alexa Fluor ® 647 antibody works fine for flow, separating the mesothelial cells from mouse lung.
Date published: 2017-04-05
Rated 1 out of 5 by from not working.haven't detected Mesothelin antigen on Mesothelin positive cell lines.
Date published: 2017-03-01
Rated 5 out of 5 by from Strong westernVery nice western blot data; strong signal, low background
Date published: 2017-01-27
Rated 5 out of 5 by from Reliable Antibody, Great for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even Flow. Strong, clear signal with little to no background.
Date published: 2017-01-12
Rated 5 out of 5 by from Used for western blotting with urine of patientsUsed for western blotting with urine of patients with ovarian or pancreatic cancer. -SCBT Publication Review
Date published: 2015-07-08
Rated 5 out of 5 by from Used for studying cardiac muscleUsed for studying cardiac muscle
Date published: 2015-05-15
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Mesothelin 抗体 (K1) is rated 4.5 out of 5 by 12.
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