Date published: 2025-9-7
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MerTK Antibody (B-1) - Western Blotting - Image 295610
MerTK 抗体 (B-1): sc-365499. 未转染 293: sc-110760 (A), 小鼠 MerTK 转染 293: sc-178930 (B) 和 Hep G2 (C) 全细胞裂解液中 MerTK 表达的 Western 印迹分析.
MerTK 抗体 (B-1): sc-365499. 未转染 293: sc-110760 (A), 小鼠 MerTK 转染 293: sc-178930 (B) 和 Hep G2 (C) 全细胞裂解液中 MerTK 表达的 Western 印迹分析.

MerTK 抗体 (B-1): sc-365499

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说明书
  • MerTK 抗体 B-1 是小鼠单克隆 IgG2b κ，MerTK抗体, 在19篇文献中引用，规格为200 µg/ml
  • 针对MerTK的human起源的N-terminal细胞外域内的氨基酸1-222
  • MerTK 抗体 (B-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MerTK
  • 抗MerTK抗体（B-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MerTK (B-1): sc-365499的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是MerTK Antibody (B-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MerTK Antibody (B-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

MerTK抗体（B-1）是一种IgG2b κ小鼠单克隆MerTK抗体（也称为MER原癌基因抗体、酪氨酸激酶抗体、C-Mer原癌基因酪氨酸激酶抗体、受体酪氨酸激酶MerTK抗体、酪氨酸蛋白激酶Mer抗体、原癌基因C-Mer抗体、Tyro12抗体、C-Eyk抗体、RP38抗体、MER抗体或STK激酶抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类的MerTK蛋白。MerTK抗体（B-1）既可作为非偶联抗MerTK抗体形式提供，也可作为多种偶联形式的抗MerTK抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。MerTK，也称为c-Mer，是Mer/Axl/Tyro3受体激酶家族的成员。它是一种由984个残基组成的跨膜蛋白，由一个酪氨酸激酶结构域、两个纤连蛋白III型结构域和两个免疫球蛋白样C2型结构域组成。MerTK是鸡逆转录病毒癌基因产物v-Eyk的哺乳动物同源物。这种蛋白质在巨噬细胞激活、血小板聚集、血栓稳定性和凋亡细胞的有效清除中起关键作用。具体而言，MerTK作为一种信号分子，在视网膜色素上皮（RPE）吞噬过程中触发外段摄取。有证据表明，MerTK通过与磷脂酰肌醇特异性磷脂酶C γ2（PI-PLC γ2）的相互作用发出信号。当编码MerTK的基因发生突变时，RPE吞噬途径会被破坏，并可能导致常染色体隐性视网膜色素变性（RP），从而导致视网膜光感受器细胞的退化。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MerTK 抗体 (B-1) 参考文献:

  1. 视网膜营养不良 RCS 大鼠的受体酪氨酸激酶基因 Mertk 突变。  |  D'Cruz, PM., et al. 2000. Hum Mol Genet. 9: 645-51. PMID: 10699188
  2. MERTK 是 RCS 大鼠视网膜营养不良基因的人类直向同源物，它的突变会导致视网膜色素变性。  |  Gal, A., et al. 2000. Nat Genet. 26: 270-1. PMID: 11062461
  3. 视网膜色素变性：受体酪氨酸激酶基因 MERTK 突变。  |  Kumar, A. 2001. J Biosci. 26: 3-5. PMID: 11255506
  4. 培养的视网膜色素上皮细胞在吞噬过程中，Mertk会触发感光体外节段的吸收。  |  Feng, W., et al. 2002. J Biol Chem. 277: 17016-22. PMID: 11861639
  5. 受体酪氨酸激酶 MerTK 在小鼠巨噬细胞识别凋亡胸腺细胞的过程中激活磷脂酶 C gamma2。  |  Todt, JC., et al. 2004. J Leukoc Biol. 75: 705-13. PMID: 14704368
  6. 原癌基因 Mer 在小儿 T 细胞急性淋巴细胞白血病中的异位表达。  |  Graham, DK., et al. 2006. Clin Cancer Res. 12: 2662-9. PMID: 16675557
  7. 由 Mertk 基因突变引起的视网膜营养不良症家族的临床特征。  |  Tschernutter, M., et al. 2006. Br J Ophthalmol. 90: 718-23. PMID: 16714263
  8. 与韩国系统性红斑狼疮患者罹患血液病风险相关的 MERTK 多态性。  |  Cheong, HS., et al. 2007. Rheumatology (Oxford). 46: 209-14. PMID: 16837475
  9. MERTK突变在遗传性视网膜疾病中的更新。  |  Audo, I., et al. 2018. Hum Mutat. 39: 887-913. PMID: 29659094
  10. KPNB1 介导的 PD-L1 核转位通过 Gas6/MerTK 信号通路促进非小细胞肺癌细胞增殖。  |  Du, W., et al. 2021. Cell Death Differ. 28: 1284-1300. PMID: 33139930
  11. 通过ADAM17和γ-分泌酶蛋白水解加工调节Mertk表面表达。  |  Lahey, KC., et al. 2024. Int J Mol Sci. 25: PMID: 38673989
  12. 新型人类原癌基因 c-mer 的克隆和 mRNA 表达分析。  |  Graham, DK., et al. 1994. Cell Growth Differ. 5: 647-57. PMID: 8086340

订购信息

产品名称产品编号规格价格数量收藏夹

MerTK 抗体 (B-1)

sc-365499
200 µg/ml
$316.00

MerTK (B-1): m-IgG Fc BP-HRP 套装

sc-529382
200 µg Ab; 10 µg BP
$354.00

MerTK (B-1): m-IgGκ BP-HRP 套装

sc-522257
200 µg Ab, 40 µg BP
$354.00

MerTK (B-1): m-IgG2b BP-HRP 套装

sc-550102
200 µg Ab; 10 µg BP
$354.00

MerTK 抗体 (B-1) AC

sc-365499 AC
500 µg/ml, 25% agarose
$416.00

MerTK 抗体 (B-1) HRP

sc-365499 HRP
200 µg/ml
$316.00

MerTK 抗体 (B-1) FITC

sc-365499 FITC
200 µg/ml
$330.00

MerTK 抗体 (B-1) PE

sc-365499 PE
200 µg/ml
$343.00

MerTK 抗体 (B-1) Alexa Fluor® 488

sc-365499 AF488
200 µg/ml
$357.00

MerTK 抗体 (B-1) Alexa Fluor® 546

sc-365499 AF546
200 µg/ml
$357.00

MerTK 抗体 (B-1) Alexa Fluor® 594

sc-365499 AF594
200 µg/ml
$357.00

MerTK 抗体 (B-1) Alexa Fluor® 647

sc-365499 AF647
200 µg/ml
$357.00

MerTK 抗体 (B-1) Alexa Fluor® 680

sc-365499 AF680
200 µg/ml
$357.00

MerTK 抗体 (B-1) Alexa Fluor® 790

sc-365499 AF790
200 µg/ml
$357.00

Do you have any reference paper showing the IF staining of MER using this antibody?

Asked by: Anonymous
Thank you for your question. We do not have any references showing IF staining of this antibody at this time, we do warranty it for IF use however. Please check back for any future updates.
Answered by: Technical Service 4
Date published: 2020-12-18

I was confused that I got the result that the MerTK Antibody (B-1) stained nucleus, how to expolain

Asked by: whmsherry
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-15

When using this antibody to do IHC, the cellular location is what, nucleus, cytosol or membrane?

Asked by: whmsherry
Thank you for your question. MerTK is a transmembrane protein that possesses three different domains: Amino acids 19 – 497 represent its extracellular topological domain. Amino acids 498 – 518 correspond to its helical transmembrane domain. Amino acids 519 – 994 form a cytoplasmic topological domain. Since MerTK Antibody (B-1): sc-365499 has been raised against the N-terminal amino acids 1 – 222, the antibody should recognize the extracellular part of MerTK.
Answered by: Tech Support Europe
Date published: 2020-07-14

can i use this antibody to mouse MerTK protein?

Asked by: eurofighter
Thank you for your question. Yes, this MerTK antibody is mouse, rat and human reactive, so it's fine for sure on mouse samples.
Answered by: Tech Service
Date published: 2019-11-26

How can I use sc-365499 to stain my mouse samples?

Asked by: Cweed
We recommend using a directly conjugated primary antibody. This product, sc-365499 is available conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 10
Date published: 2017-01-11

I am looking for a new secondary antibody to use with this since I've run out of my goat anti-mouse. What do recommend I use for my WB?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-11
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Rated 5 out of 5 by from Worked well in IHCThis antibody worked well in IHC using human samples
Date published: 2025-06-06
Rated 4 out of 5 by from Worked for Western.This worked for Western Blot application. Haven't tried anywhere else.
Date published: 2017-11-08
Rated 5 out of 5 by from Works great in FACSMerTK used in Flow Cytometry. 1*10^5 PMA-differentiated THP-1 macrophages were were stained with 2.5ul MerTK in 4% FCS/PBS for 30 minutes on ice, washed and incbuated with anti mouse secondary ab conjugated to CF(tm) 640R. Cells were read in a Accuri C6 using the FL4 sensor.
Date published: 2017-10-17
Rated 4 out of 5 by from MERTK antibody for immunohistochemistythis antibody can be used for immunohistochemisty，paraffin-embedded sections were deparaffinized with xylene and then rehydrated in a descending series of ethanol washes. Place the sections into the boil in 600 mL Tris-EDTA buffer for 20 min, and maintained at a room temperature to cool. then were blocked with 10% normal goat serum for 10 min at 37 °C. The sections incubated with theprimary antibodies to MERTK for overnight at 4 °C. renal tubule in see positive expression of brown-yellow granules.although there‘s nothing in glomerular.
Date published: 2017-10-11
Rated 5 out of 5 by from WBThis antibody could be used to de WB, and the result is good.
Date published: 2017-04-06
Rated 5 out of 5 by from Strong western dataVery nice, specific western data; strong signal, low background
Date published: 2017-01-29
Rated 5 out of 5 by from Good, versatile antibodyThe MerTK Antibody (B-1): sc-365499 works very well for multiple applications including WB, IP, IF and ELISA. This antibody has also been cited several times for further verification.
Date published: 2017-01-16
Rated 4 out of 5 by from Potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~140-205 kDa target proteins in an A431/HepG2/MCF7 cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, but the antibody can generate a slightly higher than typical background when used at 2 µg/ml. A ~52 kDa medium cross-reactive protein was detectable in the A431/HepG2/MCF7 cell lysate mix.
Date published: 2016-11-10
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MerTK 抗体 (B-1) is rated 4.6 out of 5 by 12.
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