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MEK kinase-4 抗体 (E-5): sc-166196

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说明书
  • MEK kinase-4抗体(E-5)是小鼠单克隆IgG1 κ, 在2篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1531-1607的抗原,在MEK kinase-4的C-terminus处映射,human来源
  • 推荐用于 mouse, rat 和 human 来源的MEK kinase-4 WB, IP, IF 和 ELISA检测
  • 1 BP-HRP">m-IgG1 BP-HRP是MEK kinase-4 Antibody (E-5) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与MEK kinase-4 Antibody (E-5) 打包提供(请参阅下面的订购信息)。
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有丝分裂原活化蛋白(MAP)激酶级联反应可被多种细胞外刺激物激活,包括生长因子。MEK激酶(也称为MAP激酶激酶激酶、MKKKs、MAP3Ks或MEKKs)通过磷酸化作用激活MEK(也称为MAP激酶激酶或MKKs),包括ERK、JNK和p38。这些被激活的MEK反过来又磷酸化并激活MAP激酶。MEK激酶包括Raf-1、Raf-B、Mos、MEK激酶-1、MEK激酶-2、MEK激酶-3、MEK激酶-4、ASK 1(MEK激酶-5)和MAP3K6(MEK激酶-6)。MEK激酶-1已通过非Raf依赖途径显示可磷酸化MEK-1。有证据表明,MEK-3优先被MEK激酶-3激活,而MEK-4同时被MEK激酶-2和MEK激酶-3激活。MEK激酶-4已被证明可特异性激活JNK通路。ASK1激活MEK-4和MEK-3/MEK-6通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MEK kinase-4 抗体 (E-5) 参考文献:

  1. Axin 利用不同的区域与 MEKK1 和 MEKK4 竞争性结合并激活 JNK。  |  Luo, W., et al. 2003. J Biol Chem. 278: 37451-8. PMID: 12878610
  2. GADD45beta/GADD45gamma和MEKK4共同构成一条遗传通路,在T细胞中介导STAT4非依赖性IFNgamma的产生。  |  Chi, H., et al. 2004. EMBO J. 23: 1576-86. PMID: 15044949
  3. 在角质形成细胞中,干扰素-γ 依赖性 MEKK4 通过 Pyk2 产生的酪氨酸磷酸化受 annexin II 和 SHP2 的调控。  |  Halfter, UM., et al. 2005. Biochem J. 388: 17-28. PMID: 15601262
  4. 促分裂原活化蛋白激酶激酶4(MEKK4)的缺失会导致细胞凋亡增强和神经管发育缺陷。  |  Chi, H., et al. 2005. Proc Natl Acad Sci U S A. 102: 3846-51. PMID: 15731347
  5. CIN85调节MEKK4激活p38 MAP激酶通路的能力。  |  Aissouni, Y., et al. 2005. Biochem Biophys Res Commun. 338: 808-14. PMID: 16256071
  6. MTK1/MEKK4的激活会导致心肌细胞死亡和心力衰竭。  |  Mizote, I., et al. 2010. J Mol Cell Cardiol. 48: 302-9. PMID: 19850048
  7. Gadd45β-MEKK4-p38途径对Atg5的磷酸化抑制了自噬。  |  Keil, E., et al. 2013. Cell Death Differ. 20: 321-32. PMID: 23059785
  8. TRAIL/MEKK4/p38/HSP27/Akt生存网络受Src/CIN85/c-Cbl复合物的双相调节。  |  Kim, J., et al. 2013. Cell Signal. 25: 372-9. PMID: 23085457
  9. HER2/HER3 通过 MTK1 (MEKK4) 调节细胞外酸化和细胞迁移。  |  Sollome, JJ., et al. 2014. Cell Signal. 26: 70-82. PMID: 24036211
  10. ALV-J通过GADD45β/MEKK4/P38MAPK信号通路抑制自噬,并介导自噬后的细胞凋亡。  |  Liao, Z., et al. 2020. Cell Death Dis. 11: 684. PMID: 32826872

订购信息

产品名称产品编号规格价格数量收藏夹

MEK kinase-4 抗体 (E-5)

sc-166196
200 µg/ml
$322.00

MEK kinase-4 (E-5): m-IgG1 BP-HRP 套装

sc-542657
200 µg Ab; 20 µg BP
$361.00

I need to use an antibody to detect the protein in samples of avian origin. Do you know if sc-166196: MEK kinase-4 (E-5) mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by Angeles3 from Good antibody for WBThis antibody works very well in western blotting using mouse bone marrow macrophages
Date published: 2018-09-12
Rated 5 out of 5 by SS from Nice Western Blot data of MEK kinaseNice Western Blot data of MEK kinase-4 expression in Jurkat and K-562 whole cell lysates. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by JM from Good Western Blot data of MEK kinaseGood Western Blot data of MEK kinase-4 expression in KNRK whole cell lysate. -SCBT QC
Date published: 2013-05-20
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MEK kinase-4 抗体 (E-5) is rated 5.0 out of 5 by 3.
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