Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
M-cadherin CRISPR/Cas9 KO Plasmid (h): sc-401232
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- M-cadherin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the M-cadherin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: M-cadherin Antibody (C-8): sc-398107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
M-cadherin CRISPR/Cas9 KO Plasmid (h) | sc-401232 | 20 µg | $397.00 |
Overview
CDH15 encodes M-cadherin, a calcium-dependent cell–cell adhesion receptor enriched in skeletal muscle that supports myoblast recognition, alignment, and fusion during myogenesis and muscle regeneration. Through cadherin-mediated junctions and linkage to β-catenin/p120-catenin and the actin cytoskeleton, M-cadherin contributes to contact-dependent signaling that influences cytoskeletal remodeling, migration, and differentiation programs. CDH15 activity intersects with pathways governing adherens junction dynamics and can modulate cellular responses relevant to tissue repair and remodeling. Altered expression of cadherins, including CDH15, has been associated with changes in cell adhesion and invasive behavior in disease contexts, making it relevant for studies of tumor cell–cell interactions and muscle-associated pathophysiology.
M-cadherin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDH15 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDH15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDH15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish M-cadherin protein expression.
This CRISPR knockout system enables efficient generation of CDH15-deficient cell models for investigation of M-cadherin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDH15 exon(s) critical for M-cadherin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDH15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by M-cadherin CRISPR/Cas9 KO Plasmid (h) and M-cadherin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDH15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by M-cadherin HDR Plasmid (h) and M-cadherin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDH15 homology arms to support homology-directed repair at defined CDH15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.