Date published: 2025-9-6

021-6093-6350

SCBT Portrait Logo
Seach Input

Ly-6G 抗体 (RB6-8C5): sc-53515

4.4(7)
寫評論提問

说明书
  • Ly-6G 抗体 RB6-8C5 是大鼠单克隆 IgG2bLy-6G抗体, 在38篇文献中引用,规格为200 µg/ml
  • 针对mouse来源的粒细胞
  • Ly-6G 抗体 (RB6-8C5) 推荐用于WB, IP, IF, IHC(P) 和 FCM,检测mouse 来源的 Ly-6G; 可能与 Ly-6c产生交联反应
  • 抗Ly-6G抗体(RB6-8C5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ly-6G (RB6-8C5): sc-53515免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Ly-6G 抗体 (RB6-8C5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

関連項目

Ly-6G抗体(RB6-8C5)是一种鼠单克隆IgG2b抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)检测小鼠来源的Ly-6G蛋白。Ly-6G (RB6-8C5) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。Ly-6G蛋白,也称为淋巴细胞抗原6G,是Ly-6家族中以糖基磷脂酰肌醇(GPI)为锚的蛋白质之一,其特点是富含半胱氨酸的特定结构域含有O-连接碳水化合物。Ly-6G蛋白主要表达于中性粒细胞,在细胞膜上定位,在免疫反应中发挥关键作用。值得注意的是,Ly-6G是Gr-1抗原复合物的一部分,表达于单核细胞、中性粒细胞和部分巨噬细胞,以及浆细胞样树突状细胞和T细胞。这些免疫细胞上Ly-6G的表达非常重要,因为Ly-6G参与调节免疫反应,包括抑制抗肿瘤活性和T细胞反应,这使得Ly-6G成为研究中性粒细胞生物学及其在各种病理条件下的作用的重要标记物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ly-6G 抗体 (RB6-8C5) 参考文献:

  1. 小鼠 Ly-6C 和 Ly-6G 之间不存在交叉反应。  |  Nagendra, S. and Schlueter, AJ. 2004. Cytometry A. 58: 195-200. PMID: 15057973
  2. 浸润中枢神经系统的中性粒细胞可调节 T 细胞反应。  |  Zehntner, SP., et al. 2005. J Immunol. 174: 5124-31. PMID: 15814744
  3. β-arrestin-2缺陷小鼠模型中CXCR2信号的改变。  |  Su, Y., et al. 2005. J Immunol. 175: 5396-402. PMID: 16210646
  4. 在皮肤修复的早期炎症阶段,Oncostatin M 的表达与中性粒细胞的功能有关:对正常和糖尿病受损伤口的影响。  |  Goren, I., et al. 2006. J Invest Dermatol. 126: 628-37. PMID: 16410783
  5. BALB/c 和 C57BL/6 小鼠对体外假丝酵母伯克霍尔德菌感染的先天性γ干扰素反应。  |  Koo, GC. and Gan, YH. 2006. BMC Immunol. 7: 19. PMID: 16919160
  6. 胶囊通过限制粘液介导的清除,增强肺炎球菌的定植。  |  Nelson, AL., et al. 2007. Infect Immun. 75: 83-90. PMID: 17088346
  7. CCR2 和 MCP-3 在单核细胞从骨髓动员和招募到炎症部位的过程中发挥着关键作用。  |  Tsou, CL., et al. 2007. J Clin Invest. 117: 902-9. PMID: 17364026
  8. 小鼠骨髓中髓系细胞选择性表达 Ly-6G。粒细胞分化抗原(Gr-1)的 RB6-8C5 mAb 可检测 Ly-6 家族成员。  |  Fleming, TJ., et al. 1993. J Immunol. 151: 2399-408. PMID: 8360469
  9. 两个新型 Ly-6 基因的特征。蛋白质序列以及与 alpha-bungarotoxin 和其他神经毒素的潜在结构相似性。  |  Fleming, TJ., et al. 1993. J Immunol. 150: 5379-90. PMID: 8515066

订购信息

产品名称产品编号规格价格数量收藏夹

Ly-6G 抗体 (RB6-8C5)

sc-53515
200 µg/ml
$316.00

Ly-6G 抗体 (RB6-8C5) AC

sc-53515 AC
500 µg/ml, 25% agarose
$416.00

Ly-6G 抗体 (RB6-8C5) HRP

sc-53515 HRP
200 µg/ml
$316.00

Ly-6G 抗体 (RB6-8C5) FITC

sc-53515 FITC
200 µg/ml
$330.00

Ly-6G 抗体 (RB6-8C5) PE

sc-53515 PE
200 µg/ml
$343.00

Ly-6G 抗体 (RB6-8C5) Alexa Fluor® 488

sc-53515 AF488
200 µg/ml
$357.00

Ly-6G 抗体 (RB6-8C5) Alexa Fluor® 546

sc-53515 AF546
200 µg/ml
$357.00

Ly-6G 抗体 (RB6-8C5) Alexa Fluor® 594

sc-53515 AF594
200 µg/ml
$357.00

Ly-6G 抗体 (RB6-8C5) Alexa Fluor® 647

sc-53515 AF647
200 µg/ml
$357.00

Ly-6G 抗体 (RB6-8C5) Alexa Fluor® 680

sc-53515 AF680
200 µg/ml
$357.00

Ly-6G 抗体 (RB6-8C5) Alexa Fluor® 790

sc-53515 AF790
200 µg/ml
$357.00

Will you please provide me with the protocol used to run the western blot for the Ly-6G Antibody (RB6-8C5): sc-53515 that was performed under non-reducing conditions. It would be most helpful to know the composition of the Transfer Buffer. Thank you.

Asked by: PeterMac
Thank you for your question. We followed our recommended protocol for Western blotting. Except, for the mouse PBL lysate we added our non-reducing electrophoresis buffer (sc-45085), and loaded on the regular SDS-PAGE gel without boiling. For our transfer buffer, we use sc-363874 Towbin powder, and add methanol at 10%. The bottle of Towbin powder makes 20L of 1x.
Answered by: Technical Support
Date published: 2019-08-17

How can Ly-6G (RB6-8C5): sc-53515 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: DefinitelyNotMatt
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-53515 PE) or fluoroscein (sc-53515 FITC).
Answered by: Technical Support
Date published: 2017-12-04
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_53515, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 30ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Ly-6GGood antibody for detecting Lefty in mouse gastric tissue. Dilution: 1:1000, perfect staining.
Date published: 2024-01-11
Rated 4 out of 5 by from Good antibodyWorks well on murine tissues, low background detected.
Date published: 2021-02-12
Rated 4 out of 5 by from Good product!We purchased this product a month ago and it worked nice for IHC staining.
Date published: 2018-07-10
Rated 5 out of 5 by from Great for immunohistochemical analysisI bought this antibody a month ago, and we use this antibody to mark the TANs isolated from the tumor site very well.
Date published: 2017-12-04
Rated 3 out of 5 by from Slight issue with background noiseUsing zinc-salt fixed mouse tumour section. Until now, I haven't convinced myself that the signal I've seen is actually Ly6G.
Date published: 2017-11-22
Rated 5 out of 5 by from A good antibodyI am lucky to choose the good antibody, It is really a good antibody.
Date published: 2016-11-02
Rated 5 out of 5 by from Blow out band detected in mouse PBL tissueBlow out band detected in mouse PBL tissue extract under non-reducing conditions. - SCBT QC
Date published: 2014-07-14
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_53515, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
Ly-6G 抗体 (RB6-8C5) is rated 4.4 out of 5 by 7.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_53515, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 8ms
  • REVIEWS, PRODUCT