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Luciferase Antibody (Luci 21 1-107) - Western Blotting - Image 14976
荧光素酶抗体 (Luci 21 1-107): sc-57603. Photinus pyralis 重组荧光素酶的 Western 印迹分析.
Luciferase Antibody (Luci 21 1-107) - Western Blotting - Image 14976
荧光素酶抗体 (Luci 21 1-107): sc-57603. Photinus pyralis 重组荧光素酶的 Western 印迹分析.
荧光素酶抗体 (Luci 21 1-107): sc-57603. Photinus pyralis 重组荧光素酶的 Western 印迹分析.

Luciferase 抗体 (Luci 21 1-107): sc-57603

4.3(3)
寫評論提問
说明书
  • Luciferase抗体(Luci 21 1-107)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 针对全长天然Luciferase的Photinus pyralis来源
  • 推荐用于 Photinus pyralis 来源的Luciferase WB, IP, IF 和 ELISA检测
  • 看 Luciferase (C-12): sc-74548，可查看有标记的 Luciferase 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP是Luciferase Antibody (Luci 21 1-107) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Luciferase Antibody (Luci 21 1-107) 搭配使用（请参阅下面的订购信息）。
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関連項目

荧光素酶是从北美常见的萤火虫 Photinus pyralis 中分离得到，它在生物发光生物体中起催化作用产生光，是最为广泛研究的一种酶。荧光素酶属于ATP依赖性与AMP结合的酶家族。它能产生波长为 562 nM 的绿光。下列是由荧光素酶催化的化学催化反应：Photinus荧光素+ O(2) + ATP = 氧化Photinus荧光素+ CO(2) + AMP + 二磷酸 + 光

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Luciferase 抗体 (Luci 21 1-107) 参考文献:

  1. 光萤火虫荧光素酶和大肠杆菌β-半乳糖苷酶在血液污染器官裂解液中的光度定量。  |  Smith, AD. and Trempe, JP. 2000. Anal Biochem. 286: 164-72. PMID: 11038287
  2. 一组用于体内生物发光的多色光萤火虫荧光素酶突变体。  |  Shapiro, E., et al. 2005. Protein Eng Des Sel. 18: 581-7. PMID: 16243898
  3. 光萤火虫荧光素酶中溶剂暴露的氨基酸突变可提高热稳定性和耐受pH值。  |  Law, GH., et al. 2006. Biochem J. 397: 305-12. PMID: 16551268
  4. Photinus pyralis萤火虫萤光素酶最灵活区域中的稳定性和灵活性之间的关系。  |  Amini-Bayat, Z., et al. 2012. Biochim Biophys Acta. 1824: 350-8. PMID: 22155276
  5. 萤火虫和蛾萤火虫的嵌合体荧光素酶产生增强的生物发光。  |  Branchini, BR., et al. 2014. Biochemistry. 53: 6287-9. PMID: 25264115
  6. 萤火虫荧光素酶基因：结构和在哺乳动物细胞中的表达。  |  de Wet, JR., et al. 1987. Mol Cell Biol. 7: 725-37. PMID: 3821727
  7. 亚洲萤火虫Pygoluciola qingyu荧光素酶的克隆和特性描述，以及与其他甲虫荧光素酶的比较。  |  Li, J., et al. 2024. Photochem Photobiol Sci. 23: 719-729. PMID: 38441849
  8. 对潜在入侵萤火虫Photinus signaticollis Blanchard 1845的基因组学研究：完整的线粒体基因组、多基因系统发育以及萤光素酶和荧光素再生基因的获取。  |  Koken, M. and Gastineau, R. 2024. Arthropod Struct Dev. 82: 101384. PMID: 39288692
  9. 手性控制碳量子点诱导的萤火虫荧光素酶的结构和活性变化。  |  Rad-Faraji, M., et al. 2024. Nanoscale Adv. 6: 6154-65. PMID: 39386119
  10. 萤火虫（光萤火虫）荧光素酶的折叠：未折叠中间体的聚集和再激活。  |  Herbst, R., et al. 1998. Biochemistry. 37: 6586-97. PMID: 9572876

订购信息

产品名称产品编号规格价格数量收藏夹

Luciferase 抗体 (Luci 21 1-107)

sc-57603
200 µg/ml
$322.00

Luciferase (Luci 21 1-107): m-IgG Fc BP-HRP 套装

sc-539064
200 µg Ab; 10 µg BP
$361.00

I want to use luciferase antibody for detecting N terminal domain of luciferase. What protein sequence Luciferase (Luci21 1-107) does target ? Is this antibody useful for detecting only N terminal domain of luciferase ?

Asked by: regret
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-08-17

What is the recommended starting dilution for Western blot for sc-57603: Luciferase (Luci 21 1-107) monoclonal antibody?

Asked by: jerojero
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by Wordsworth from goodGood release data in the mouse MHV-68WB was a great help to my experiment and would be considered again.
Date published: 2017-01-17
Rated 4 out of 5 by QZ from Strong band in WB in Photinus pyralis recombinantStrong band in WB in Photinus pyralis recombinant Luciferase. -SCBT QC
Date published: 2015-03-07
Rated 4 out of 5 by NZ from Good publishable data in WB in mouse MHVGood publishable data in WB in mouse MHV-68 (WT or M3FL infected). -SCBT Publication Review
Date published: 2013-04-01
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Luciferase 抗体 (Luci 21 1-107) is rated 4.3 out of 5 by 3.
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