Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
LL-37 Antibody (D-5) - Immunohistochemistry - Image 159513
LL-37 抗体 (D-5): sc-166770. 福尔马林固定, 石蜡包埋的人脾组织的免疫过氧化物酶染色显示红髓细胞的胞浆和膜染色.
LL-37 抗体 (D-5): sc-166770. 福尔马林固定, 石蜡包埋的人脾组织的免疫过氧化物酶染色显示红髓细胞的胞浆和膜染色.

LL-37 抗体 (D-5): sc-166770

5.0(7)
寫評論提問

说明书
  • LL-37 抗体 D-5 是小鼠单克隆 IgG1 κ，LL-37抗体, 在31篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列131-170位于human物种的LL-37 proteolytic fragment of CAP-18的C-terminus
  • LL-37 抗体 (D-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 mature LL-37
  • 抗LL-37抗体（D-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LL-37 (D-5): sc-166770的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 LL-37 抗体 (D-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LL-37 抗体 (D-5) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 LL-37 & CAP-18 抗体包括 CAP-18 和 LL-37

LL-37抗体（D-5）是一种IgG1 κ小鼠单克隆LL-37抗体（也被称为抗菌肽前体衍生物LL-37抗体、巨噬细胞/多形核白细胞（PMN）特异性溶酶体定位肽LL37抗体，或CAP-18（CAMP）前体翻译后裂解产物LL37特异性抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人类来源的LL-37蛋白。LL-37抗体（D-5）既有非偶联抗LL-37抗体形式，也有多种偶联形式的抗LL-37抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。抗菌肽是一类在中性粒细胞过氧化物酶阴性颗粒中发现的抗菌蛋白。抗菌肽与防御素蛋白家族一起，通过预防局部感染和系统性的微生物入侵，参与第一道防线。CAP-18前体（FALL-39肽抗生素、阳离子抗菌蛋白、CAMP、HSD26）是一种含有抗菌肽LL-37（氨基酸134-170）的抗菌肽蛋白。与富含半胱氨酸、折叠成β-折叠片的防御素不同，LL-37是一种不含半胱氨酸的肽，可以采用两亲性α-螺旋构象。LL-37与细菌脂多糖（LPS）结合，是一种有效的趋化因子，可募集肥大细胞至炎症部位。LL-37存在于包括银屑病、亚急性红斑狼疮、皮炎和镍接触性过敏在内的炎症性皮肤病中。在正常皮肤表皮中未发现。该分泌蛋白主要在骨髓、睾丸和中性粒细胞中表达。小鼠和大鼠的正交基因CRAMP（cathelin相关抗菌肽）也是宿主防御肽抗菌肽家族的一部分。其中包括强效抗菌肽的前体，这些前体直接对各种微生物病原体产生抗菌活性，并在伤口愈合过程中激活间充质细胞。CRAMP在睾丸、脾脏、胃和肠中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LL-37 抗体 (D-5) 参考文献:

  1. 人类 cathelicidin（hCAP-18）通过蛋白酶 3 在细胞外裂解加工成抗菌肽 LL-37。  |  Sørensen, OE., et al. 2001. Blood. 97: 3951-9. PMID: 11389039
  2. 人类抗菌肽 LL-37 中的一种猫肝菌素家族可诱导肥大细胞趋化。  |  Niyonsaba, F., et al. 2002. Immunology. 106: 20-6. PMID: 11972628
  3. 唾液腺和唾液中都表达有猫肽抗菌肽。  |  Murakami, M., et al. 2002. J Dent Res. 81: 845-50. PMID: 12454100
  4. 小鼠和人类新生儿皮肤表达的抗菌肽水平增加：适应性反应发育过程中的先天免疫。  |  Dorschner, RA., et al. 2003. Pediatr Res. 53: 566-72. PMID: 12612195
  5. 大鼠 cathelicidin rCRAMP 的系统发育, 加工和表达：先天性抗菌肽模型。  |  Termén, S., et al. 2003. Cell Mol Life Sci. 60: 536-49. PMID: 12737313
  6. cathelicidins--结构, 功能和进化。  |  Tomasinsig, L. and Zanetti, M. 2005. Curr Protein Pept Sci. 6: 23-34. PMID: 15638766
  7. LL-37 转运免疫活性 cGAMP，激活 STING 信号，增强干扰素介导的宿主抗病毒免疫力。  |  Wei, X., et al. 2022. Cell Rep. 39: 110880. PMID: 35649354
  8. LL-37是一种参与NETosis的多方面两性肽。  |  Radic, M. and Muller, S. 2022. Cells. 11: PMID: 35954305
  9. LL-37 在骨和牙周再生中的调节作用  |  Chinipardaz, Z., et al. 2022. Life (Basel). 12: PMID: 36294968
  10. 一种在骨髓中表达的新型小鼠 cathelin 样蛋白。  |  Popsueva, AE., et al. 1996. FEBS Lett. 391: 5-8. PMID: 8706928
  11. 鉴定 CRAMP，一种在胚胎和成年小鼠中表达的 cathelin 相关抗菌肽。  |  Gallo, RL., et al. 1997. J Biol Chem. 272: 13088-93. PMID: 9148921
  12. 人类角质细胞在发生炎症时会诱导抗菌肽 LL-37 的编码基因表达。  |  Frohm, M., et al. 1997. J Biol Chem. 272: 15258-63. PMID: 9182550

订购信息

产品名称产品编号规格价格数量收藏夹

LL-37 抗体 (D-5)

sc-166770
200 µg/ml
$316.00

LL-37 (D-5): m-IgG Fc BP-HRP 套装

sc-527185
200 µg Ab; 10 µg BP
$354.00

LL-37 (D-5): m-IgG1 BP-HRP 套装

sc-532558
200 µg Ab; 20 µg BP
$354.00

LL-37 抗体 (D-5) AC

sc-166770 AC
500 µg/ml, 25% agarose
$416.00

LL-37 抗体 (D-5) HRP

sc-166770 HRP
200 µg/ml
$316.00

LL-37 抗体 (D-5) FITC

sc-166770 FITC
200 µg/ml
$330.00

LL-37 抗体 (D-5) PE

sc-166770 PE
200 µg/ml
$343.00

LL-37 抗体 (D-5) Alexa Fluor® 488

sc-166770 AF488
200 µg/ml
$357.00

LL-37 抗体 (D-5) Alexa Fluor® 546

sc-166770 AF546
200 µg/ml
$357.00

LL-37 抗体 (D-5) Alexa Fluor® 594

sc-166770 AF594
200 µg/ml
$357.00

LL-37 抗体 (D-5) Alexa Fluor® 647

sc-166770 AF647
200 µg/ml
$357.00

LL-37 抗体 (D-5) Alexa Fluor® 680

sc-166770 AF680
200 µg/ml
$357.00

LL-37 抗体 (D-5) Alexa Fluor® 790

sc-166770 AF790
200 µg/ml
$357.00

We are using this Ab (LL-37 (D-5): sc-166770) along with m-IgGk-BP-HRP (2ndry Ab as recommended) to analyze protein expression in cancer cell lines (positive control HeLa cells), but having difficulty to see any signal. Any suggestions? thanks

Asked by: SeemaD
Thank you for your question. We recommend using this primary antibody at a starting dilution of 1:200 and loading 40-60 µg of protein. A link to our complete recommended protocol for Western blot is provided below. LL-37 is a short secreted peptide (~4.5 kDa) that is difficult to detect in Western blot. We have not tested this antibody in HeLa cells, so we do not know how abundant LL-37 is in this cell line. We are aware of publications citing use of this antibody in Western blot and we would recommend reviewing these references for more detailed information about using the antibody (e.g., PMID: 25330301 & 23940207). Please contact our Technical Service Department for additional support. Please call us toll-free at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-14

Can this mouse primary antibody be used for IF or IHC staining in mouse tissue?

Asked by: Anna99
Yes. LL-37 Antibody (D-5): sc-166770, is available conjugated to HRP (sc-166770 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-166770 PE), fluorescein (sc-166770 FITC), Alexa Fluor® 488 (sc-166770 AF488) or Alexa Fluor® 647 (sc-166770 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 13
Date published: 2017-01-09
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_166770, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 99ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from LL-37 antibody - the only one that workedWe brought LL-37 antibodies from other companies to detected LL-37 in bronchial epithelial cells and this monoclonal from Santa Cruz (D-5) was the only one that worked for Western blot analysis
Date published: 2017-12-01
Rated 5 out of 5 by from goodwe applicate it into the ICC of HGECs and it works well
Date published: 2017-02-25
Rated 5 out of 5 by from Outstanding WB and IHCVery clear western blot results; strong signal; also great IHC data
Date published: 2017-01-27
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-12
Rated 5 out of 5 by from WB publishable data with human carcinomaWB publishable data with human carcinoma - SCBT Publication Review
Date published: 2015-04-26
Rated 5 out of 5 by from Strong band in western blot analysis of humanStrong band in western blot analysis of human recombinant CAMP. -SCBT QC
Date published: 2015-02-08
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human spleen tissue. -SCBT QC
Date published: 2014-11-03
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_166770, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
LL-37 抗体 (D-5) is rated 5.0 out of 5 by 7.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_zh_CN, sid_166770, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 98ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。