Lipocalin-15 CRISPR/Cas9 KO Plasmid (h): sc-415842
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Lipocalin-15 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Lipocalin-15 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Lipocalin-15 CRISPR/Cas9 KO Plasmid (h) | sc-415842 | 20 µg | $397.00 |
Overview
LCN15 encodes lipocalin-15, a member of the lipocalin family of secreted proteins that bind and transport small hydrophobic ligands, including lipids and retinoid-like metabolites. Through ligand sequestration and delivery, lipocalin-15 is positioned to influence extracellular signaling, membrane homeostasis, and intercellular communication in tissue microenvironments. Altered expression of lipocalins has been associated with inflammation, metabolic stress responses, and epithelial remodeling, making LCN15 a useful target for dissecting lipid-handling and stress-adaptive pathways. Studying LCN15 function can inform mechanisms linking secreted carrier proteins to paracrine regulation and disease-associated changes in cellular state.
Lipocalin-15 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the LCN15 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the LCN15 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the LCN15 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Lipocalin-15 protein expression.
This CRISPR knockout system enables efficient generation of LCN15-deficient cell models for investigation of Lipocalin-15 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting LCN15 exon(s) critical for Lipocalin-15 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple LCN15 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Lipocalin-15 CRISPR/Cas9 KO Plasmid (h) and Lipocalin-15 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the LCN15 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Lipocalin-15 HDR Plasmid (h) and Lipocalin-15 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by LCN15 homology arms to support homology-directed repair at defined LCN15 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.