Date published: 2025-9-9

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LEF-1 抗体 (B-6): sc-374522

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说明书
  • LEF-1抗体(B-6)是小鼠单克隆IgG1 κ, 在15篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列61-130位于human物种的LEF-1的N-terminus附近
  • 抗-LEF-1 抗体 (B-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 LEF-1
  • 抗LEF-1抗体(B-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-374522 X, 200 µg/0.1 ml)
  • 关于如何获取LEF-1 (B-6): sc-374522免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 LEF-1 抗体 (B-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与LEF-1 抗体 (B-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

LEF-1抗体(B-6)是一种IgG1 κ小鼠单克隆LEF-1抗体,可通过WB、IP、IF、IHC(P)和ELISA方法检测小鼠、大鼠和人源LEF-1蛋白。LEF-1抗体(B-6)既可以是非偶联的抗LEF-1抗体形式,也可以是多种偶联形式的抗LEF-1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。TCF(T细胞因子)家族的转录因子由Wnt-1和Wingless途径激活,其特征是存在一个保守的蛋白质基序,即高迁移率组(HMG)1盒,它介导DNA结合。TCF(T细胞因子)蛋白在发育过程中是必需的,包括对于淋巴细胞发育至关重要的TCF-1,以及参与神经元发育和LEF(白血病增强因子)的TCF-3和TCF-4。Wnt介导的信号通路诱导细胞质中的β-连环蛋白与细胞核内的TCF蛋白结合,从而增强Wnt靶向基因的表达。β-连环蛋白-TCF复合物受到腺瘤性结肠息肉(APC)肿瘤抑制蛋白的负调控,该蛋白使β-连环蛋白磷酸化,进而增加细胞质中β-连环蛋白的降解,从而抑制TCF蛋白的转录活性。APC基因的突变在结直肠癌中常见,会破坏这一调控途径,并与β-连环蛋白的积累和TCF靶向基因的激活增加相关联。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

LEF-1 抗体 (B-6)

sc-374522
200 µg/ml
$316.00

LEF-1 (B-6): m-IgG Fc BP-HRP 套装

sc-525974
200 µg Ab; 10 µg BP
$354.00

LEF-1 抗体 (B-6) X

sc-374522 X
200 µg/0.1 ml
$316.00

LEF-1 抗体 (B-6) AC

sc-374522 AC
500 µg/ml, 25% agarose
$416.00

LEF-1 抗体 (B-6) HRP

sc-374522 HRP
200 µg/ml
$316.00

LEF-1 抗体 (B-6) FITC

sc-374522 FITC
200 µg/ml
$330.00

LEF-1 抗体 (B-6) PE

sc-374522 PE
200 µg/ml
$343.00

LEF-1 抗体 (B-6) Alexa Fluor® 488

sc-374522 AF488
200 µg/ml
$357.00

LEF-1 抗体 (B-6) Alexa Fluor® 546

sc-374522 AF546
200 µg/ml
$357.00

LEF-1 抗体 (B-6) Alexa Fluor® 594

sc-374522 AF594
200 µg/ml
$357.00

LEF-1 抗体 (B-6) Alexa Fluor® 647

sc-374522 AF647
200 µg/ml
$357.00

LEF-1 抗体 (B-6) Alexa Fluor® 680

sc-374522 AF680
200 µg/ml
$357.00

LEF-1 抗体 (B-6) Alexa Fluor® 790

sc-374522 AF790
200 µg/ml
$357.00

Could this antibody work for FA fixed chromatin immunoprecipitation?

Asked by: Anonymous
Thank you for your question. For use in ChIP we recommend using LEF-1 Anticorpo (B-6) X, as the gelatin in our standard solution could interfere with the experiment.
Answered by: Tech Support Europe
Date published: 2021-07-14

this antibody recognizes all LEF1 isoforms ? if it does, do you have any antibody against lef1 short isoforms (dominant negative isoforms) ? thanks !

Asked by: JoanaG
Thank you for your question. LEF-1 Antibody (B-6): sc-374522 has been raised against amino acids 61-130 mapping near the N-terminus of LEF-1 of human origin. It recognizes isoforms 1 (canonical), 2, 5, and 6. Since the amino acid sequences 1-115 are missing in isoforms 3 and 4, and 1-70 is missing in isoform 7, the antibody may not recognize these variants. LEF-1 Antibody (B-10): sc-374412 has been raised against amino acids 61-130 mapping near the N-terminus of LEF-1 of human origin as well. Please contact our technical service for further information or to arrange for a sample of the antibodies.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I am using LEF-1 (B-6): sc-374522 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from no good signal in IFI didn't detect good staining signal in 4%PFA fixed embryonic mouse brain(after antigen retrieval)
Date published: 2018-03-07
Rated 5 out of 5 by from good for IHCnuclear staining, 1:100 dilution, sodium citrate antigen retrieval
Date published: 2018-02-13
Rated 5 out of 5 by from Good antibodyThis antibody works well at 1:500 dilution for WB.
Date published: 2018-02-09
Rated 4 out of 5 by from Good antibodyImmunofluorescence (1:200) works well on frozen section from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0) is necessary.
Date published: 2017-10-03
Rated 5 out of 5 by from Good choice for IHC(P) and WBExcellent results in both IHC on paraffin sections and in Western blotting, strong, clean and specific.
Date published: 2017-01-19
Rated 5 out of 5 by from Great immunoperoxidase nuclear staining inGreat immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-03-01
Rated 5 out of 5 by from Great Western Blot data of LEFGreat Western Blot data of LEF-1 expression in non-transfected and human LEF-1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-06-10
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LEF-1 抗体 (B-6) is rated 4.4 out of 5 by 7.
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