Date published: 2025-9-6
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LAMP-2 Antibody (ABL-93) - Western Blotting - Image 4904
LAMP-2 抗体 (ABL-93): sc-20004. NIH/3T3 (A), RAW 264. 7 (B), J774. A1 (C) 和 Sol8 (D) 全细胞裂解液中 LAMP-2 表达的 Western 印迹分析.
LAMP-2 抗体 (ABL-93): sc-20004. NIH/3T3 (A), RAW 264. 7 (B), J774. A1 (C) 和 Sol8 (D) 全细胞裂解液中 LAMP-2 表达的 Western 印迹分析.

LAMP-2 抗体 (ABL-93): sc-20004

4.3(16)
寫評論提問

说明书
  • LAMP-2 抗体 ABL-93 是大鼠单克隆 IgG2a, 在43篇文献中引用，提供 200 µg/ml
  • 针对从BALB/c mouse胚胎3T3细胞系纯化的糖蛋白部分
  • 抗-LAMP-2 抗体 (ABL-93) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 LAMP-2
  • 抗LAMP-2抗体（ABL-93）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联Alexa Fluor® 405 用于IF, IHC(P) 和FCM
  • 关于如何获取LAMP-2 (ABL-93): sc-20004的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成LAMP-2 抗体 (ABL-93)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 LAMP 抗体 包括 LAMP-1, LAMP-2和LAMP-5

LAMP-2抗体（ABL-93）是一种鼠单克隆IgG2a抗体，针对从BALB/c小鼠胚胎3T3细胞系纯化的糖蛋白部分，在小鼠、大鼠和人类中均具有反应性。抗LAMP-2抗体（ABL-93）经过验证可用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）在内的多种应用。LAMP-2，也称为CD107B，是一种溶酶体相关膜糖蛋白，在溶酶体功能和细胞稳态中起着至关重要的作用。LAMP-2是一种I型跨膜蛋白，有助于维持溶酶体的完整性、酸度，并保护溶酶体膜免受降解。 LAMP-2还参与自噬，调节自噬体与溶酶体的融合，这对于细胞成分的降解和回收至关重要。 LAMP-2失调与溶酶体贮积症（如达农病）有关，并可能导致癌症和其他疾病中溶酶体功能的改变。LAMP-2在跨高尔基体网络中进行分类，并在细胞内运输，这凸显了其在细胞运输和溶酶体生物发生中的作用。LAMP-2单克隆抗体（ABL-93）是研究LAMP-2在溶酶体维持、自噬和疾病过程中的功能的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

LAMP-2 抗体 (ABL-93)

sc-20004
200 µg/ml
$316.00

LAMP-2 抗体 (ABL-93) AC

sc-20004 AC
500 µg/ml, 25% agarose
$416.00

LAMP-2 抗体 (ABL-93) HRP

sc-20004 HRP
200 µg/ml
$316.00

LAMP-2 抗体 (ABL-93) FITC

sc-20004 FITC
200 µg/ml
$330.00

LAMP-2 抗体 (ABL-93) PE

sc-20004 PE
200 µg/ml
$343.00

LAMP-2 抗体 (ABL-93) Alexa Fluor® 488

sc-20004 AF488
200 µg/ml
$357.00

LAMP-2 抗体 (ABL-93) Alexa Fluor® 546

sc-20004 AF546
200 µg/ml
$357.00

LAMP-2 抗体 (ABL-93) Alexa Fluor® 594

sc-20004 AF594
200 µg/ml
$357.00

LAMP-2 抗体 (ABL-93) Alexa Fluor® 647

sc-20004 AF647
200 µg/ml
$357.00

LAMP-2 抗体 (ABL-93) Alexa Fluor® 680

sc-20004 AF680
200 µg/ml
$357.00

LAMP-2 抗体 (ABL-93) Alexa Fluor® 790

sc-20004 AF790
200 µg/ml
$357.00

LAMP-2 抗体 (ABL-93) Alexa Fluor® 405

sc-20004 AF405
200 µg/ml
$357.00

Can LAMP-2 (ABL-93): sc-20004 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, LAMP-2 (ABL-93) is available in a variety of direct conjugations, such as HRP, PE, FITC, Alexa Fluor® 405, AlexaFluor 488 and AlexaFluor 647.
Answered by: Technical Support 6
Date published: 2021-02-09
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Rated 1 out of 5 by from no signalI tried this antibody at different concentration for WB on mouse cortex lysates and it didn't work, no signal.
Date published: 2021-11-25
Rated 5 out of 5 by from wbuse it to text the autophasy signal in the primary neuron cell of rats,and it worked
Date published: 2017-12-13
Rated 3 out of 5 by from I don't think this is a right pattern for LAMP2I got the sample sack two weeks ago. Antibody for detecting mouse specifical LAMP2 is not easy to look for. Although I got the same signal as in the product description, I don't think it is a right pattern for LAMP2.
Date published: 2017-09-19
Rated 3 out of 5 by from can detect signal but not effeciencecan detect signal but not effecience。 the signal is enough strong
Date published: 2017-09-05
Rated 1 out of 5 by from Did not work for brain lysateSuppose to be a negative control in brain lysate in western blot, but band did not show up
Date published: 2017-08-10
Rated 5 out of 5 by from works ok for IHC\DC 2.4 cells (murine) cells. It however worked with the 2' AB specifically that can recognize IgG2b form. It did not work well in my hand with IgG (H+L) 2' Abs
Date published: 2017-07-16
Rated 5 out of 5 by from good result of WBI use this antibody detect mosue brain tissue expression the target protein.we recommend transferred the protein onto PVDF membranes with a semi-dry transfer method,and check and confirm the proteinis transfer to the membrance successfully,then incubate the antibody with the membrance for 1hour at RT ,the expose time shoudnot be too long,it may cause dark background.
Date published: 2017-07-01
Rated 5 out of 5 by from Clear bandThis antibody works well for WB, diluted with 1/600, shows good quality
Date published: 2017-05-10
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LAMP-2 抗体 (ABL-93) is rated 4.3 out of 5 by 16.
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