Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
L3MBTL3 CRISPR/Cas9 KO Plasmid (h): sc-413649
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- L3MBTL3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the L3MBTL3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: L3MBTL3 Antibody (F-9): sc-518224
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
L3MBTL3 CRISPR/Cas9 KO Plasmid (h) | sc-413649 | 20 µg | $397.00 |
Overview
Human L3MBTL3 encodes a member of the malignant brain tumor (MBT) domain family of chromatin readers that recognize methyl-lysine marks and contribute to transcriptional repression. L3MBTL3 participates in epigenetic regulation by linking histone mark interpretation to chromatin compaction and gene silencing programs that influence cell cycle control, differentiation, and maintenance of genome stability. It has been implicated in hematopoietic biology and broader transcriptional networks where altered chromatin regulation can reshape lineage decisions and proliferative capacity. Dysregulation of MBT-domain chromatin readers, including L3MBTL3, is relevant to studies of oncogenic transcriptional states and other diseases driven by epigenetic imbalance.
L3MBTL3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the L3MBTL3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the L3MBTL3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the L3MBTL3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish L3MBTL3 protein expression.
This CRISPR knockout system enables efficient generation of L3MBTL3-deficient cell models for investigation of L3MBTL3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting L3MBTL3 exon(s) critical for L3MBTL3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple L3MBTL3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by L3MBTL3 CRISPR/Cas9 KO Plasmid (h) and L3MBTL3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the L3MBTL3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by L3MBTL3 HDR Plasmid (h) and L3MBTL3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by L3MBTL3 homology arms to support homology-directed repair at defined L3MBTL3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.