Date published: 2025-9-6
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KV4.2 Antibody (H-5) - Western Blotting - Image 289583
KV4. 2 抗体 (H-5): sc-390571. 大鼠大脑 (A), 小鼠大脑 (B) 和人类海马 (C) 组织提取物中 KV4. 2 表达的 Western 印迹分析.
KV4.2 Antibody (H-5) - Western Blotting - Image 289583
KV4. 2 抗体 (H-5): sc-390571. 大鼠大脑 (A), 小鼠大脑 (B) 和人类海马 (C) 组织提取物中 KV4. 2 表达的 Western 印迹分析.
KV4.2 Antibody (H-5) - Western Blotting - Image 304566
KV4. 2 抗体 (H-5): sc-390571. 未经处理 (A) 和λ蛋白磷酸酶 (sc-200312A) 处理 (B) 的人脑组织提取物中 KV4. 2 磷酸化的 Western 印迹分析.
KV4. 2 抗体 (H-5): sc-390571. 大鼠大脑 (A), 小鼠大脑 (B) 和人类海马 (C) 组织提取物中 KV4. 2 表达的 Western 印迹分析.

KV4.2 抗体 (H-5): sc-390571

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说明书
  • KV4.2 抗体 H-5 是小鼠单克隆 IgG1 κ，KV4.2抗体, 在2篇文献中引用，规格为200 µg/ml
  • 免疫human来源KV4.2位于C-terminal细胞质域的406-630氨基酸
  • KV4.2 抗体 (H-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 KV4.2; 也和以下物种反应，包括： porcine
  • 抗KV4.2抗体（H-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取KV4.2 (H-5): sc-390571的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是KV4.2 Antibody (H-5) for WB applications. 的首选辅助检测试剂，这些试剂现与KV4.2 Antibody (H-5) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

此外 KV 抗体包括 和

KV4.2抗体（H-5）是一种IgG1 κ小鼠单克隆KV4.2抗体（也称为KV4.2抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的KV4.2蛋白。KV4.2抗体（H-5）既有非偶联抗KV4.2抗体形式，也有多种偶联形式的抗KV4.2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。质膜中的电压门控K+通道控制着神经元、肌肉和其他可兴奋细胞的动作电位的复极和频率。KV基因家族编码30多个基因，这些基因构成K+通道的亚单位，它们在门控和渗透特性、亚细胞分布和表达模式上有所不同。功能性的KV通道是由构成孔道的α-亚单位（KVα）组成的四聚体，包括KV1、KV2、KV3和KV4蛋白，以及修饰共表达的KVα亚单位门控特性的辅助或KVβ亚单位。大鼠和人脑表达的主要KV1亚单位（KV1.1、KV1.2、KV1.4、KV1.5和KV1.6）在转运、生物合成处理和表面表达模式上存在差异，这表明单个蛋白质亚单位受到高度调控，以控制功能性神经元通道的组装和形成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

KV4.2 抗体 (H-5) 参考文献:

  1. 人类中枢神经系统 Kv1 通道的亚基组成  |  Coleman, SK., et al. 1999. J Neurochem. 73: 849-58. PMID: 10428084
  2. 亚基组成决定了 Kv1 钾通道的表面表达。  |  Manganas, LN. and Trimmer, JS. 2000. J Biol Chem. 275: 29685-93. PMID: 10896669
  3. Kv4.2基因敲除小鼠心肌细胞中存在编码为Kv4.3的快速瞬态外向电流  |  Liu, J., et al. 2015. PLoS One. 10: e0133274. PMID: 26196737
  4. Kv4.2 基因敲除小鼠在拉什利迷宫中表现出学习和记忆缺陷。  |  Smith, GD., et al. 2016. F1000Res. 5: 2456. PMID: 28163893
  5. Pin1 对 Kv4.2 的活动依赖性异构化可调节认知灵活性。  |  Hu, JH., et al. 2020. Nat Commun. 11: 1567. PMID: 32218435
  6. 类脂质病神经元 3 蛋白 CLN3 对 Kv4.2/KChIP3 相互作用的调控  |  Seifert, C., et al. 2020. J Biol Chem. 295: 12099-12110. PMID: 32641494
  7. 电压依赖性钾通道Kv4.2通过激活神经发生减轻缺血性中风损伤  |  Xiao, F., et al. 2021. Neurochem Int. 150: 105155. PMID: 34384853
  8. 通过串联亲和纯化-质谱法鉴定原代神经元中的 Kv4.2 蛋白复合物和修饰。  |  Hu, JH., et al. 2022. Front Cell Neurosci. 16: 1070305. PMID: 36568885
  9. 脑 Kv1.1 钾通道：亚基组装和翻译后加工的体外和体内研究。  |  Deal, KK., et al. 1994. J Neurosci. 14: 1666-76. PMID: 8126562
  10. Kv1 通道亚基五个成员的免疫组化定位：大鼠大脑中不同的亚细胞位置和神经元特异性共定位。  |  Veh, RW., et al. 1995. Eur J Neurosci. 7: 2189-205. PMID: 8563969
  11. Beta 亚基通过生物合成早期的效应促进 K+ 通道表面的表达。  |  Shi, G., et al. 1996. Neuron. 16: 843-52. PMID: 8608002
  12. 哺乳动物脑 K+ 通道复合物中 Kvbeta1 和 Kvbeta2 β-亚基与 Kv1 α-亚基的缔合作用和共定位。  |  Rhodes, KJ., et al. 1997. J Neurosci. 17: 8246-58. PMID: 9334400

订购信息

产品名称产品编号规格价格数量收藏夹

KV4.2 抗体 (H-5)

sc-390571
200 µg/ml
$316.00

KV4.2 (H-5): m-IgG Fc BP-HRP 套装

sc-527639
200 µg Ab; 10 µg BP
$354.00

KV4.2 (H-5): m-IgG1 BP-HRP 套装

sc-533012
200 µg Ab; 20 µg BP
$354.00

KV4.2 抗体 (H-5) AC

sc-390571 AC
500 µg/ml, 25% agarose
$416.00

KV4.2 抗体 (H-5) HRP

sc-390571 HRP
200 µg/ml
$316.00

KV4.2 抗体 (H-5) FITC

sc-390571 FITC
200 µg/ml
$330.00

KV4.2 抗体 (H-5) PE

sc-390571 PE
200 µg/ml
$343.00

KV4.2 抗体 (H-5) Alexa Fluor® 488

sc-390571 AF488
200 µg/ml
$357.00

KV4.2 抗体 (H-5) Alexa Fluor® 546

sc-390571 AF546
200 µg/ml
$357.00

KV4.2 抗体 (H-5) Alexa Fluor® 594

sc-390571 AF594
200 µg/ml
$357.00

KV4.2 抗体 (H-5) Alexa Fluor® 647

sc-390571 AF647
200 µg/ml
$357.00

KV4.2 抗体 (H-5) Alexa Fluor® 680

sc-390571 AF680
200 µg/ml
$357.00

KV4.2 抗体 (H-5) Alexa Fluor® 790

sc-390571 AF790
200 µg/ml
$357.00

Is there any issue in using sc-390571 for detection of the short isoform of Kv4.3 (Uniprot: Q9UK17-2)? Do you have any data showing detection of the above mentioned isoform in Western blot experiment?

Asked by: janclod
Thank you for your question. We have not tested this mouse monoclonal, KV4.2/4.3 Antibody (H-5): sc-390571, to evaluate potential reactivity with the long and short isoforms of the protein. All Western blot data for this antibody is shown on our website. This antibody is raised against amino acids 406-630 of KV4.2 of human origin. Based on sequence analysis, the immunogen does share only 59% sequence identity with the short isoform of Kv4.3 (Q9UK17-2). The exact epitope recognized by this antibody has not been determined, but based on this alignment there is a possibility that the antibody could recognize both the long and short isoforms.
Answered by: Technical Support
Date published: 2017-03-30

Are there any issues with using sc-390571: KV4.2/4.3 (H-5) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-390571 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from Very reliable antibody for a tricky protein!The KCND2/3 (Kv4.2/4.3) gene translates into proteins of theoretical molecular weight approximately 70 and 73kDa in Rat respectively. I have tried this antibody in rat cortex and hippo-campus tissue and found a 75 kDa band which is equivalent to Kv4.3/4.2 proteins (see my blot picture). The 50 kDa band may be coming from he secondary antibody because I see that in other antibodies too occasionally (time to change the secondary I guess). the higher molecular weight bands (>250kDa) which appeared as smear and possibly post translation modified form of Kv4.2/4.3, membrane proteins are highly post transnational modified ( Glycosylated etc) which may cause a huge shift in mol weight. Another thing is that the membrane proteins need special lysis buffer, they are meant to be associated with lipids, so regular RIPA buffer may not be always good to get them in dissolved state and sometimes heating tend to precipitate membrane proteins. so someone may miss the high molecular (>250) smeared bands because of his/her choice of buffer, but in this case I have used a special detergent and did not boiled the sample. so this blot opens up new questions and have the potential for a summer student project. anyway buy this antibody and it will not disappoint you. who knows, an extra band may lead to a great discovery. Cheers!
Date published: 2017-04-08
Rated 5 out of 5 by from Satisfactory Western Blot data of KV4Satisfactory Western Blot data of KV4.2/4.3 expression in rat brain, mouse brain and human hippocampus tissue extracts. -SCBT QC
Date published: 2013-08-13
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KV4.2 抗体 (H-5) is rated 5.0 out of 5 by 2.
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