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KSP37 Antibody (D-2): sc-365803

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Datasheets
  • KSP37 Antibody (D-2) is a mouse monoclonal IgG3 κ KSP37 antibody provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 21-50 near the N-terminus of KSP37 of human origin
  • recommended for detection of KSP37 of human origin by WB, IP, IF, IHC(P) and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for KSP37 Antibody (D-2). This work is in progress.
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    KSP37 (D-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects KSP37 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). Anti-KSP37 antibody (D-2) is available as non-conjugated form, as well as multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. KSP37 (37 kDa killer-specific secretory protein), also known as FGFBP2 (fibroblast growth factor binding protein 2), is a 223 amino acid protein secreted into extracellular space and belongs to fibroblast growth factor-binding protein family. KSP37 is expressed in serum, cytotoxic T lymphocytes and peripheral leukocytes, and is thought to be involved in lymphocyte-mediated immunity, possibly playing a role in asthma development. The gene encoding KSP37 maps to human chromosome 4, which encodes nearly 6% of human genome and has largest gene deserts (regions of genome with no protein encoding genes) of all human chromosomes. Defects in some genes located on chromosome 4 are associated with Huntington′s disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    KSP37 Antibody (D-2)

    sc-365803
    200 µg/ml
    $322.00

    Can you please advise me on the best methodology to use this antibody in IF assay using paraffin embedded human kidney samples? I´ve tried 1:100 and 1:50 dilution and i) citrate ph6; ii) pH9 and iii) PK, as antigen recovery methods without success.Thanks.

    Asked by: Anonymous
    Thank you for your question. You can find our recommended protocol with recommendations for antigen retrieval options under this link: https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
    Answered by: Tech Support Europe
    Date published: 2020-10-08

    What application is the blocking peptide sc-365803 P appropriate for?

    Asked by: jerojero
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-24
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    Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skeletal muscle tissue. -SCBT QC
    Date published: 2015-04-23
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    KSP37 Antibody (D-2) is rated 5.0 out of 5 by 2.
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