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KPI-2 抗体 (D-11): sc-398396

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说明书
  • KPI-2 抗体 D-11 是小鼠单克隆 IgG1 (kappa轻链),KPI-2抗体,规格为200 µg/ml
  • 免疫原氨基酸序列15-148位于human物种的KPI-2的N-terminus附近
  • KPI-2 抗体 (D-11) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 KPI-2
  • 抗KPI-2抗体(D-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取KPI-2 (D-11): sc-398396免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是KPI-2 Antibody (D-11) for WB applications. 的首选辅助检测试剂,这些试剂现与KPI-2 Antibody (D-11) 打包提供(请参阅下面的订购信息)。

    快捷链接

    関連項目

    KPI-2抗体(D-11)是一种IgG1 κ小鼠单克隆KPI-2抗体(也被称为KPI-2抗体),可通过WB、IP、IF和ELISA等方法检测人类来源的KPI-2蛋白。KPI-2抗体(D-11)既有非偶联形式的KPI-2抗体,也有多种偶联形式的KPI-2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。KPI-2(激酶/磷酸酶/抑制剂2),也被称为BREK、LMR2、cprk、AATYK2或LMTK2(狐猴酪氨酸激酶2),是一种含有1503个氨基酸的单通道膜蛋白,属于蛋白激酶超家族和蛋白酪氨酸激酶家族。KPI-2主要在骨骼肌中表达,在脑和胰腺中表达较弱,KPI-2含有N端跨膜螺旋和一个长的C端细胞质尾,具有丝氨酸/苏氨酸/酪氨酸激酶活性。KPI-2可能参与神经生长因子(NGF)-TrkA信号传导、内体膜转运和精子生成。KPI-2定位于细胞质膜囊泡和核周循环内体。KPI-2对于内吞膜囊泡从早期内体向循环内体的转变至关重要。编码KPI-2的基因是前列腺癌的潜在治疗靶点。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    KPI-2 抗体 (D-11) 参考文献:

    1. 一种新型跨膜 Ser/Thr 激酶与蛋白磷酸酶-1 和抑制剂-2 复合。  |  Wang, H. and Brautigan, DL. 2002. J Biol Chem. 277: 49605-12. PMID: 12393858
    2. 大脑中富含的丝氨酸/苏氨酸激酶 BREK 参与了 NGF 信号的传递。  |  Kawa, S., et al. 2004. Genes Cells. 9: 219-32. PMID: 15005709
    3. 对跨膜 Ser/Thr 激酶 KPI-2 底物特异性的肽芯片分析表明,它与囊性纤维化跨膜传导调节因子和磷酸化酶有反应。  |  Wang, H. and Brautigan, DL. 2006. Mol Cell Proteomics. 5: 2124-30. PMID: 16887929
    4. 定向干扰 Brek/Lmtk2(脑丰富激酶/lemur 酪氨酸激酶 2)基因的小鼠无精子症。  |  Kawa, S., et al. 2006. Proc Natl Acad Sci U S A. 103: 19344-9. PMID: 17158803
    5. 肌球蛋白VI及其相互作用蛋白LMTK2调控小管的形成和向内细胞循环区的运输。  |  Chibalina, MV., et al. 2007. J Cell Sci. 120: 4278-88. PMID: 18029400
    6. 新发现的与前列腺癌易感性相关的多个基因位点。  |  Eeles, RA., et al. 2008. Nat Genet. 40: 316-21. PMID: 18264097
    7. BREK/LMTK2 是一种肌球蛋白 VI 结合蛋白,参与内体膜的转运。  |  Inoue, T., et al. 2008. Genes Cells. 13: 483-95. PMID: 18429820
    8. 确定前列腺癌的新遗传风险因素。  |  Guy, M., et al. 2009. Asian J Androl. 11: 49-55. PMID: 19050691
    9. LMTK2和PARP-2基因多态性与继发于减数分裂停滞的无精子症。  |  Sakugawa, N., et al. 2009. J Assist Reprod Genet. 26: 545-52. PMID: 19806447

    订购信息

    产品名称产品编号规格价格数量收藏夹

    KPI-2 抗体 (D-11)

    sc-398396
    200 µg/ml
    $316.00

    KPI-2 (D-11): m-IgG Fc BP-HRP 套装

    sc-527771
    200 µg Ab; 10 µg BP
    $354.00

    KPI-2 (D-11): m-IgG1 BP-HRP 套装

    sc-533144
    200 µg Ab; 20 µg BP
    $354.00

    KPI-2 抗体 (D-11) AC

    sc-398396 AC
    500 µg/ml, 25% agarose
    $416.00

    KPI-2 抗体 (D-11) HRP

    sc-398396 HRP
    200 µg/ml
    $316.00

    KPI-2 抗体 (D-11) FITC

    sc-398396 FITC
    200 µg/ml
    $330.00

    KPI-2 抗体 (D-11) PE

    sc-398396 PE
    200 µg/ml
    $343.00

    KPI-2 抗体 (D-11) Alexa Fluor® 488

    sc-398396 AF488
    200 µg/ml
    $357.00

    KPI-2 抗体 (D-11) Alexa Fluor® 546

    sc-398396 AF546
    200 µg/ml
    $357.00

    KPI-2 抗体 (D-11) Alexa Fluor® 594

    sc-398396 AF594
    200 µg/ml
    $357.00

    KPI-2 抗体 (D-11) Alexa Fluor® 647

    sc-398396 AF647
    200 µg/ml
    $357.00

    KPI-2 抗体 (D-11) Alexa Fluor® 680

    sc-398396 AF680
    200 µg/ml
    $357.00

    KPI-2 抗体 (D-11) Alexa Fluor® 790

    sc-398396 AF790
    200 µg/ml
    $357.00

    What is the recommended fixation method for immunofluorescence staining with KPI-2 (D-11): sc-398396 monoclonal antibody?

    Asked by: DefinitelyNotMatt
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-02-28
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    Rated 3 out of 5 by from good featuresI applied for the samples several days ago and they gave it to me immediately. very good and sensitive.
    Date published: 2018-01-31
    Rated 5 out of 5 by from Good immunoprecipitationI used this antibody to pull down LMTK2, and it worked great.
    Date published: 2017-07-06
    Rated 5 out of 5 by from Product quality is guaranteedThree months ago I bought this antibody, the results is very beautiful.A good antibody can save a lot of time to experiment,the company's antibody is trustworthy.
    Date published: 2016-12-07
    Rated 4 out of 5 by from Highly potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~250 kDa target protein in a HeLa/Jurkat cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested. A ~55 kDa medium cross-reactive protein was detected in the HeLa/Jurkat cell lysate mix.
    Date published: 2016-11-10
    Rated 5 out of 5 by from Satisfactory Western Blot data of KPISatisfactory Western Blot data of KPI-2 expression in HeLa, ARPE-19 and ES-2 whole cell lysates. -SCBT QC
    Date published: 2013-02-20
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    KPI-2 抗体 (D-11) is rated 4.4 out of 5 by 5.
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