KIAA0664 Double Nickase Plasmid (h): sc-407383-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIAA0664 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- KIAA0664 Double Nickase Plasmid (h) and KIAA0664 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLUH. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIAA0664 Double Nickase Plasmid (h) | sc-407383-NIC | 20 µg | $410.00 | |||
KIAA0664 Double Nickase Plasmid (h2) | sc-407383-NIC-2 | 20 µg | $410.00 |
Human CLUH (KIAA0664) encodes a cytosolic RNA-binding protein that coordinates mitochondrial biogenesis by regulating the localization, stability, and translation of nuclear-encoded mRNAs for mitochondrial proteins. CLUH supports oxidative phosphorylation capacity and mitochondrial distribution by coupling post-transcriptional control to organelle dynamics, helping maintain energy homeostasis during proliferation and stress responses. Disruption of CLUH-dependent mRNA regulation can alter mitochondrial morphology, respiratory chain function, and cellular metabolism, processes frequently interrogated in studies of neurodegeneration, cardiometabolic dysfunction, and cancer cell bioenergetics. As a hub for mitochondria-associated RNA granules, KIAA0664 is also used to probe links between RNA metabolism, proteostasis, and mitochondrial quality control pathways.
KIAA0664 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLUH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLUH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLUH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLUH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.