KIAA0664 CRISPR/Cas9 KO Plasmid (h): sc-407383
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- KIAA0664 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the KIAA0664 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
KIAA0664 CRISPR/Cas9 KO Plasmid (h) | sc-407383 | 20 µg | $397.00 |
Overview
CLUH (also known as KIAA0664) encodes a cytosolic RNA-binding protein that couples mitochondrial biogenesis with spatial organization of mitochondria by regulating the stability, localization, and translation of nuclear-encoded mRNAs for mitochondrial proteins. It contributes to oxidative phosphorylation capacity and cellular energy homeostasis, influencing processes such as stress responses and metabolic adaptation through control of mitochondria-associated gene expression programs. Disruption of CLUH-linked mitochondrial regulation can perturb respiratory function, alter mitochondrial distribution, and impact pathways governing cell growth and survival. Accordingly, CLUH is relevant to research on mitochondrial dysfunction and metabolism-associated disease mechanisms, including contexts where altered bioenergetics and organelle dynamics are hallmarks.
KIAA0664 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CLUH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CLUH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CLUH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish KIAA0664 protein expression.
This CRISPR knockout system enables efficient generation of CLUH-deficient cell models for investigation of KIAA0664 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CLUH exon(s) critical for KIAA0664 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CLUH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by KIAA0664 CRISPR/Cas9 KO Plasmid (h) and KIAA0664 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CLUH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by KIAA0664 HDR Plasmid (h) and KIAA0664 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CLUH homology arms to support homology-directed repair at defined CLUH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.