Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Josephin-3 (TATA box-binding protein-associated factor RNA polymerase I subunit D) is a 278 amino acid transcription factor encoded by the human TAF1D gene. The TAF1D gene product is a major component of a TBP and TAF's (TATA box-binding protein-associated factors) complex known as SL1. This SL1 complex is important for the assembly of the preinitiation complex required for RNA polymerase I-dependent transcription. The SL1/TIF-IB complex has been shown to stabilize the nucleolar transcription factor 1/UBTF on rDNA. Impaired function of the SL1 complex leads to reduced levels of Pol I transcription. The TAF1D gene product has been shown to be involved in cell cycle progression and exhibits G2/M phase specific phosphorylations. The TAF1D gene product is expressed in most tissues and is localized to the nucleus.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
How much sample do you recommend loading on the gel for Western blot detection using Josephin-3 (B-10): sc-515158?
Asked by: Professor Griffin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: