JMJD5 Double Nickase Plasmid (h): sc-405787-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- JMJD5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- JMJD5 Double Nickase Plasmid (h) and JMJD5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting KDM8. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: JMJD5 Antibody (D-5): sc-377078
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
JMJD5 Double Nickase Plasmid (h) | sc-405787-NIC | 20 µg | $410.00 | |||
JMJD5 Double Nickase Plasmid (h2) | sc-405787-NIC-2 | 20 µg | $410.00 |
KDM8 (JMJD5) encodes a Jumonji C domain–containing dioxygenase that functions as an epigenetic regulator linking chromatin state to transcriptional control. JMJD5 has been implicated in histone and non-histone substrate hydroxylation/demethylation activities and modulates processes including cell-cycle progression, DNA damage responses, and metabolic gene expression. Through interactions with transcriptional and chromatin-associated complexes, JMJD5 contributes to regulation of hypoxia- and stress-responsive pathways and coordination of proliferative programs. Dysregulated JMJD5 activity or expression has been associated with altered growth control and genome maintenance, making KDM8 a useful target for mechanistic studies in cancer biology and related disorders.
JMJD5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the KDM8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within KDM8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt KDM8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of KDM8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.