ITI-H4 Antibody (F-9): sc-515353

ITI-H4 Antibody (F-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects ITI-H4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-ITI-H4 antibody (F-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The inter-α-trypsin inhibitor (ITI) family, to which ITI-H4 belongs, consists of structurally related plasma serine protease inhibitors synthesized in the liver, comprising different combinations of three highly homologous heavy chains (ITI-HI, ITI-H2, and ITI-H3) and one light chain (Bikunin). Notably, ITI-H4 features a proline-rich region (PRR) at its C-terminus, which may play a role in functional properties. The ITI family is a glycoprotein composed of three polypeptides linked by chondroitin sulfate: two heavy chains, ITI-H1 and ITI-H2, and Bikunin, with the latter conferring the protease-inhibitor function of ITI. The heavy chains, known as serum-derived hyaluronan-associated proteins (SHAPs), bind covalently to hyaluronic acid, contributing to the stabilization of the pericellular matrix. Importantly, while the ITI family is primarily composed of multi-polypeptide molecules, ITI-H4 is unique as a single-chain molecule. The gene encoding human ITI-H4 is located on chromosome 3p21.1, and interestingly, the gene transcriptions and products for rat and human ITI-H4 exhibit significant differences, indicating potential species-specific functions for this protein.

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ITI-H4 Antibody (F-9) References:

1. ITIH4 serum concentration increases during acute-phase processes in human patients and is up-regulated by interleukin-6 in hepatocarcinoma HepG2 cells.  |  Piñeiro, M., et al. 1999. Biochem Biophys Res Commun. 263: 224-9. PMID: 10486281
2. Itih-4, a serine protease inhibitor regulated in interleukin-6-dependent liver formation: role in liver development and regeneration.  |  Bhanumathy, CD., et al. 2002. Dev Dyn. 223: 59-69. PMID: 11803570
3. Hypercholesterolemia associated with splice-junction variation of inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) gene.  |  Fujita, Y., et al. 2004. J Hum Genet. 49: 24-28. PMID: 14661079
4. The concentrations of EGFR, LRG1, ITIH4, and F5 in serum correlate with the number of colonic adenomas in ApcPirc/+ rats.  |  Ivancic, MM., et al. 2014. Cancer Prev Res (Phila). 7: 1160-9. PMID: 25200834
5. Long Non-coding RNA ITIH4-AS1 Accelerates the Proliferation and Metastasis of Colorectal Cancer by Activating JAK/STAT3 Signaling.  |  Liang, C., et al. 2019. Mol Ther Nucleic Acids. 18: 183-193. PMID: 31557619
6. ITIH4, as an inflammation biomarker, mainly increases in bacterial bloodstream infection.  |  Ma, Y., et al. 2021. Cytokine. 138: 155377. PMID: 33348064
7. ITIH4 acts as a protease inhibitor by a novel inhibitory mechanism.  |  Pihl, R., et al. 2021. Sci Adv. 7: PMID: 33523981
8. ITIH4 is a novel serum biomarker for early gastric cancer diagnosis.  |  Sun, Y., et al. 2021. Clin Chim Acta. 523: 365-373. PMID: 34687700
9. Prioritization of human well-being spectrum related GWAS-SNVs using ENCODE-based web-tools predict interplay between PSMC3, ITIH4, and SERPINC1 genes in modulating well-being.  |  Pyne, T., et al. 2021. J Psychiatr Res. 145: 92-101. PMID: 34883412
10. PGK1 modulates balance between pro- and anti-inflammatory cytokines by interacting with ITI-H4.  |  Park, HB., et al. 2023. Biomed Pharmacother. 161: 114437. PMID: 36841032

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ITI-H4 Antibody (F-9) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-ITI-H4 Antibody (F-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ITI-H4 Antibody (F-9) Specifications

Alternate Product Name: ITI-H4

Product Clone Name: F-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 891-920

Epitope Target: ITI-H4

Epitope Species: human

Product Reactivity/Detection: full length and 35 kDa fragment of ITI-H4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for ITI-H4 Antibody (F-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred ITI-H4 antibody is ITI-H4 Antibody (G-2): sc-515060.