Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ISYNA1 CRISPR/Cas9 KO Plasmid (h): sc-404785
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ISYNA1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ISYNA1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ISYNA1 Antibody (C-9): sc-271830
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ISYNA1 CRISPR/Cas9 KO Plasmid (h) | sc-404785 | 20 µg | $397.00 |
Overview
ISYNA1 (inositol-3-phosphate synthase 1) encodes the rate-limiting enzyme that converts glucose-6-phosphate to myo-inositol-3-phosphate, providing a key entry point into de novo myo-inositol biosynthesis. Myo-inositol supplies precursors for phosphatidylinositol and phosphoinositide signaling lipids that regulate membrane trafficking, receptor-linked signaling, cytoskeletal dynamics, and intracellular calcium homeostasis. Through its contribution to PI/PI(4,5)P2/PI(3,4,5)P3 metabolism, ISYNA1 influences pathways such as PI3K–AKT and PLC-driven signaling that shape proliferation, stress responses, and differentiation. Altered inositol and phosphoinositide homeostasis has been implicated in metabolic and neurodevelopmental phenotypes, making ISYNA1 a useful target for mechanistic studies of signaling and membrane dynamics.
ISYNA1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ISYNA1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ISYNA1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ISYNA1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ISYNA1 protein expression.
This CRISPR knockout system enables efficient generation of ISYNA1-deficient cell models for investigation of ISYNA1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ISYNA1 exon(s) critical for ISYNA1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ISYNA1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ISYNA1 CRISPR/Cas9 KO Plasmid (h) and ISYNA1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ISYNA1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ISYNA1 HDR Plasmid (h) and ISYNA1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ISYNA1 homology arms to support homology-directed repair at defined ISYNA1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.