ISGF-3γ p48 CRISPR/Cas9 KO Plasmid (m): sc-421156
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ISGF-3γ p48 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ISGF-3γ p48 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ISGF-3γ p48 CRISPR/Cas9 KO Plasmid (m) | sc-421156 | 20 µg | $397.00 |
Overview
Irf9 encodes ISGF-3γ p48 (also known as IRF9), a DNA-binding component of the interferon-stimulated gene factor 3 (ISGF3) complex that partners with STAT1 and STAT2 to drive transcription of interferon-stimulated genes. In mouse cells, IRF9 is central to type I and type III interferon signaling, linking JAK–STAT activation to antiviral restriction programs, antigen presentation, and broader innate immune regulation. Through modulation of ISG networks, IRF9 influences inflammatory tone, cytokine responsiveness, and immune cell communication in tissues. Dysregulated IRF9/ISGF3 activity is frequently studied in contexts of viral susceptibility, autoimmunity-associated interferon signatures, and inflammation-driven pathology relevant to cancer and metabolic disease models.
ISGF-3γ p48 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Irf9 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Irf9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Irf9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ISGF-3γ p48 protein expression.
This CRISPR knockout system enables efficient generation of Irf9-deficient cell models for investigation of ISGF-3γ p48 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Irf9 exon(s) critical for ISGF-3γ p48 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Irf9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ISGF-3γ p48 CRISPR/Cas9 KO Plasmid (m) and ISGF-3γ p48 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Irf9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ISGF-3γ p48 HDR Plasmid (m) and ISGF-3γ p48 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Irf9 homology arms to support homology-directed repair at defined Irf9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.