Date published: 2025-9-6

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IL-3Rα 抗体 (C-5): sc-74522

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说明书
  • IL-3R alpha 抗体 C-5 是小鼠单克隆 IgG1, 在3篇文献中引用,提供 200 µg/ml
  • 针对IL-3Rα的mouse起源的N-terminal细胞外域内的氨基酸17-210
  • IL-3R alpha 抗体 (C-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测mouse 来源的 IL-3Rα
  • 抗IL-3R alpha抗体(C-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IL-3Rα (C-5): sc-74522免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成IL-3Rα 抗体 (C-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

IL-3Rα抗体(C-5)是一种小鼠单克隆IgG1抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测小鼠来源的IL-3Rα。IL-3Rα (C-5) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。白细胞介素-3受体α(IL-3Rα)通过介导白细胞介素-3的作用在造血中发挥关键作用,白细胞介素-3是一种促进造血祖细胞增殖和分化的细胞因子。IL-3Rα位于细胞表面,与常见的β亚基(IL-3Rβ)形成异二聚体,对于高亲和力IL-3结合至关重要。IL-3和IL-3Rα相互作用会启动细胞内信号传导通路,包括JAK2激酶的激活,从而导致Stat5a和Stat5b转录因子的磷酸化。IL-3Rα信号支持血细胞的存活和增殖,因此IL-3Rα对于免疫反应和血液疾病至关重要。IL-3Rα的独特结构特征(包括低亲和力结合特性)能够微调造血反应,凸显了IL-3Rα在正常生理和疾病状态中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IL-3Rα 抗体 (C-5)

sc-74522
200 µg/ml
$316.00

IL-3Rα 抗体 (C-5) AC

sc-74522 AC
500 µg/ml, 25% agarose
$416.00

IL-3Rα 抗体 (C-5) HRP

sc-74522 HRP
200 µg/ml
$316.00

IL-3Rα 抗体 (C-5) FITC

sc-74522 FITC
200 µg/ml
$330.00

IL-3Rα 抗体 (C-5) PE

sc-74522 PE
200 µg/ml
$343.00

IL-3Rα 抗体 (C-5) Alexa Fluor® 488

sc-74522 AF488
200 µg/ml
$357.00

IL-3Rα 抗体 (C-5) Alexa Fluor® 546

sc-74522 AF546
200 µg/ml
$357.00

IL-3Rα 抗体 (C-5) Alexa Fluor® 594

sc-74522 AF594
200 µg/ml
$357.00

IL-3Rα 抗体 (C-5) Alexa Fluor® 647

sc-74522 AF647
200 µg/ml
$357.00

IL-3Rα 抗体 (C-5) Alexa Fluor® 680

sc-74522 AF680
200 µg/ml
$357.00

IL-3Rα 抗体 (C-5) Alexa Fluor® 790

sc-74522 AF790
200 µg/ml
$357.00

What is the host of this antibody?

Asked by: Mona
Thank you for your question. IL-3Rα Antibody (C-5): sc-74522 is a monoclonal IgG1 raised in mouse.
Answered by: Tech Support Europe
Date published: 2024-11-18

For use in IHC paraffin, antigen retrieval what it is recommended?

Asked by: Lorena Alonso
Thank you for your message. Please find on the following link our highly recommended protocol: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining If you have further questions, please do not hesitate to contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-11-27

I am interested in trying this antibody. I am confused by the example staining, however. I have not seen any reports of hepatocytes expressing IL-3Rα and the staining looks non-specific. Would it be possible to try a sample? 

Asked by: nsorr
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-11-22

Can IL-3Rα (C-5): sc-74522 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, IL-3Rα (C-5): sc-74522 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-25
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Rated 1 out of 5 by from No staining for immunohistochemistryWe tried to stain human cells using an automated immunostainer with the concentration 1:100, but no staining at all.
Date published: 2020-09-23
Rated 3 out of 5 by from Used in IHCStain in both human and mouse tissues, some background observed in staining
Date published: 2017-10-19
Rated 5 out of 5 by from Nice Western blot data of mouse recombinant ILNice Western blot data of mouse recombinant IL-3Ra. -SCBT QC
Date published: 2014-04-20
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IL-3Rα 抗体 (C-5) is rated 3.0 out of 5 by 3.
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