IMP-1/IGF2BP1 Antibody (D-9): sc-166344

IGF2BP1 Antibody (D-9) is a mouse monoclonal IgM antibody that detects IGF2BP1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-IGF2BP1 antibody (D-9) is available as a non-conjugated format. The Insulin-like Growth Factor 2 mRNA Binding Protein 1 (IGF2BP1), also known as coding region determinant-binding protein (CRD-BP), plays a crucial role in regulating RNA metabolism and stability, which is vital for cellular growth and differentiation. IGF2BP1 is predominantly located in the cytoplasm, where IGF2BP1 binds to specific mRNAs, including those for c-Myc and IGF-II, thereby influencing their translation and stability. IGF2BP1 presence in embryonal tissues highlights IGF2BP1′s importance in developmental processes, while IGF2BP1 overexpression in various cancers, such as colon, sarcoma, and breast cancer, suggests a role in tumorigenesis. IGF2BP1′s unique structure, characterized by multiple RNA recognition motifs and hnRNP K homology domains, enables IGF2BP1 to interact with a variety of RNA targets, thereby modulating gene expression in response to cellular signals. This multifaceted role in RNA binding and regulation underscores IGF2BP1′s significance in both normal physiology and disease states, making IGF2BP1 (D-9) antibody an essential tool for researchers studying cancer biology and RNA metabolism.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

IMP-1/IGF2BP1 Antibody (D-9) References:

1. C-MYC and IGF-II mRNA-binding protein (CRD-BP/IMP-1) in benign and malignant mesenchymal tumors.  |  Ioannidis, P., et al. 2001. Int J Cancer. 94: 480-4. PMID: 11745432
2. 8q24 Copy number gains and expression of the c-myc mRNA stabilizing protein CRD-BP in primary breast carcinomas.  |  Ioannidis, P., et al. 2003. Int J Cancer. 104: 54-9. PMID: 12532419
3. Targeted knockdown of the RNA-binding protein CRD-BP promotes cell proliferation via an insulin-like growth factor II-dependent pathway in human K562 leukemia cells.  |  Liao, B., et al. 2004. J Biol Chem. 279: 48716-24. PMID: 15355996
4. Effect of all-trans-retinoic acid on mRNA binding protein p62 in human gastric cancer cells.  |  Ping, S., et al. 2005. Int J Biochem Cell Biol. 37: 616-27. PMID: 15618018
5. The RNA-binding protein IMP-3 is a translational activator of insulin-like growth factor II leader-3 mRNA during proliferation of human K562 leukemia cells.  |  Liao, B., et al. 2005. J Biol Chem. 280: 18517-24. PMID: 15753088
6. CRD-BP/IMP1 expression characterizes cord blood CD34+ stem cells and affects c-myc and IGF-II expression in MCF-7 cancer cells.  |  Ioannidis, P., et al. 2005. J Biol Chem. 280: 20086-93. PMID: 15769738
7. Developmental regulation of CRD-BP, an RNA-binding protein that stabilizes c-myc mRNA in vitro.  |  Leeds, P., et al. 1997. Oncogene. 14: 1279-86. PMID: 9178888

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-IMP-1/IGF2BP1 Antibody (D-9) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-IMP-1/IGF2BP1 Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

IMP-1/IGF2BP1 Antibody (D-9) Specifications

Alternate Product Name: ZBP1

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 324-367

Epitope Target: IGF2BP1

Epitope Species: human

Product Reactivity/Detection: IGF2BP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for IMP-1/IGF2BP1 Antibody (D-9): P: Blocking Peptide