Date published: 2025-9-6
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IFN-γ/Interferon gamma Antibody (E-10) - Western Blotting - Image 146558
IFN-γ (E-10): sc-373727. 人重组 IFN-γ 融合蛋白的 Western 印迹分析.
IFN-γ/Interferon gamma Antibody (E-10) - Western Blotting - Image 146558
IFN-γ (E-10): sc-373727. 人重组 IFN-γ 融合蛋白的 Western 印迹分析.
IFN-γ (E-10): sc-373727. 人重组 IFN-γ 融合蛋白的 Western 印迹分析.

IFN-γ/Interferon gamma 抗体 (E-10): sc-373727

3.5(6)
寫評論提問

说明书
  • IFN-γ/Interferon gamma 抗体 E-10 是小鼠单克隆 IgG2a κ，IFN-γ/Interferon gamma抗体, 在15篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的IFN-γ的N-terminus的氨基酸25-50之间
  • IFN-γ/Interferon gamma 抗体 (E-10) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 precursor and mature IFN-γ
  • 抗IFN-γ/Interferon gamma抗体（E-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IFN-γ/Interferon gamma (E-10): sc-373727的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是IFN-γ/Interferon gamma Antibody (E-10) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与IFN-γ/Interferon gamma Antibody (E-10) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
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说明书与实验方案
研究信息

関連項目

附加IFN抗体，包括IFN-α, IFN-α 1, IFN-α 13, IFN-α 2, IFN-β, IFN-γ和IFN-ω

IFN-γ抗体（E-10）是一种IgG2a κ小鼠单克隆IFN-gamma抗体（也称为IFNG抗体、干扰素gamma抗体、免疫干扰素抗体、IFN gamma抗体或干扰素-γ抗体），可通过WB、IP、IF和ELISA检测人源IFN-gamma蛋白。 IFN-γ抗体（E-10）既可作为非偶联抗IFN-gamma抗体形式，也可作为多种偶联形式的抗IFN-gamma抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。 干扰素(IFN)-γ是一种由CD4+/CD8+淋巴细胞和自然杀伤细胞产生的抗病毒和抗寄生虫剂，这些细胞通过抗原、有丝分裂原或同种异型抗原进行激活。 IFN-γ的产生可调节T细胞的生长和分化，并抑制B细胞的生长。 IFN-γ的合成可通过IL-2、FGF和EGF诱导。 IFN-γ的活性形式是同型二聚体，每个亚基包含六个螺旋。 人IFN-γ的二聚体结构通过螺旋界面的非共价相互作用而稳定。 IFN-γ翻译前体是166个氨基酸，包括23个氨基酸的分泌序列。 由于可变的糖基化和非变性条件，存在多种形式，包括二聚体和四聚体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IFN-γ/Interferon gamma 抗体 (E-10)

sc-373727
200 µg/ml
$316.00

IFN-γ/Interferon gamma (E-10): m-IgGκ BP-HRP 套装

sc-522473
200 µg Ab, 40 µg BP
$354.00

IFN-γ/Interferon gamma 抗体 (E-10) AC

sc-373727 AC
500 µg/ml, 25% agarose
$416.00

IFN-γ/Interferon gamma 抗体 (E-10) HRP

sc-373727 HRP
200 µg/ml
$316.00

IFN-γ/Interferon gamma 抗体 (E-10) FITC

sc-373727 FITC
200 µg/ml
$330.00

IFN-γ/Interferon gamma 抗体 (E-10) PE

sc-373727 PE
200 µg/ml
$343.00

IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor® 488

sc-373727 AF488
200 µg/ml
$357.00

IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor® 546

sc-373727 AF546
200 µg/ml
$357.00

IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor® 594

sc-373727 AF594
200 µg/ml
$357.00

IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor® 647

sc-373727 AF647
200 µg/ml
$357.00

IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor® 680

sc-373727 AF680
200 µg/ml
$357.00

IFN-γ/Interferon gamma 抗体 (E-10) Alexa Fluor® 790

sc-373727 AF790
200 µg/ml
$357.00

IFN-γ/Interferon gamma (E-10) 中和勝肽

sc-373727 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-373727 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-28
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Rated 3 out of 5 by from better results with sc-8423pref. the sc-8423 product over this when working with mouse samples.
Date published: 2018-03-23
Rated 1 out of 5 by from Holy (and expensive) waterTried the free IFNgamma (E-10) in Western blot (1:100 as suggested by datasheet) with olfactory mesenchymal stem cells (oMSC) and mouse cortex protein extracts to check for signal in these two models. Not a single band was detected. Everything was done properly. I have tested 8 (eight!) mouse monoclonal antibodies from Santa Cruz and not a single one gave any band except one that gave an aspecific signal (I would like to upload a picture, but it is not allowing me). I will not loose more time with them and I strongly advice you to do the same. Good luck, fellow researchers.
Date published: 2017-11-17
Rated 3 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by from A GOOD PRODUCTI used the antibody in western blot, and to test the mice liver samples. So, that's good!
Date published: 2017-09-19
Rated 5 out of 5 by from very usefulIFN-gama antibody effect is good, immunohistochemical staining uniform, positive coloring significantly.
Date published: 2017-07-21
Rated 5 out of 5 by from Produced positive Western blot data of humanProduced positive Western blot data of human recombinant IFN- fusion protein. -SCBT QC
Date published: 2014-03-15
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IFN-γ/Interferon gamma 抗体 (E-10) is rated 3.5 out of 5 by 6.
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