Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
HPRT Antibody (F-1) - Western Blotting - Image 299286
HPRT 抗体 (F-1): sc-376938. Hep G2 (A), KNRK (B), HeLa (C), A-431 (D) 和 MCF7 (E) 全细胞裂解液中 HPRT 表达的 Western 印迹分析.
HPRT 抗体 (F-1): sc-376938. Hep G2 (A), KNRK (B), HeLa (C), A-431 (D) 和 MCF7 (E) 全细胞裂解液中 HPRT 表达的 Western 印迹分析.

HPRT 抗体 (F-1): sc-376938

4.9(8)
寫評論提問

说明书
  • HPRT 抗体 F-1 是小鼠单克隆 IgG1 κ，HPRT抗体, 在25篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的HPRT的C-terminus附近的氨基酸167-201之间
  • HPRT 抗体 (F-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HPRT; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗HPRT抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HPRT (F-1): sc-376938的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成HPRT 抗体 (F-1)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

HPRT抗体（F-1）是一种IgG1κ小鼠单克隆HPRT抗体（也称为次黄嘌呤磷酸核糖转移酶1抗体、HGPRT抗体、次黄嘌呤-鸟嘌呤磷酸核糖转移酶抗体、HGPRTase抗体、次黄嘌呤-鸟嘌呤磷酸核糖转移酶1抗体、睾丸组织蛋白Li 89抗体或莱希-尼汉综合征抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的HPRT蛋白。 HPRT抗体（F-1）可以以非偶联抗HPRT抗体形式和多种偶联抗HPRT抗体形式提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。 HPRT（次黄嘌呤磷酸核糖转移酶1），也称为HGPRT或HPRT1，是一种218个氨基酸的细胞质蛋白，属于嘌呤/嘧啶磷酸核糖转移酶家族。参与嘌呤代谢，HPRT作为嘌呤救济酶发挥作用，催化次黄嘌呤和鸟嘌呤转化为各自的单核苷酸（分别为一磷酸肌苷和一磷酸鸟苷）。 HPRT以同四聚体形式存在，可以结合两个镁离子作为辅因子。编码HPRT的基因缺陷是痛风和莱希-尼汉综合征（LNS）的原因，这两种疾病均以NPRT酶活性部分或完全缺失为特征。虽然HPRT酶活性部分丧失导致尿酸积聚（痛风），但酶活性完全丧失导致高尿酸血症、精神发育迟滞、舞蹈手足徐动症和强迫性自残，这些都是与LNS相关的症状。这些疾病的严重程度表明HPRT在嘌呤代谢中起着重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HPRT 抗体 (F-1) 参考文献:

  1. 与莱施-奈恩综合征相关的次黄嘌呤磷酸核糖转移酶（HPRT1）基因中反复出现的大阿鲁介导的缺失。  |  Mizunuma, M., et al. 2001. Hum Mutat. 18: 435-43. PMID: 11668636
  2. 与人类 X 连锁 HPRT 基因中基质连接区相关的非活性 X 特异性复制原点。  |  Koina, E. and Piper, A. 2005. J Cell Biochem. 95: 391-402. PMID: 15779006
  3. 一名因 HPRT 缺乏症而患有痛风的男性的正常 HPRT 编码区。  |  Dawson, PA., et al. 2005. Mol Genet Metab. 85: 78-80. PMID: 15862284
  4. HPRTSardinia：一种新的点突变导致 HPRT 缺乏症，但不伴有莱希-奈恩病。  |  Cossu, A., et al. 2006. Biochim Biophys Acta. 1762: 29-33. PMID: 16216473
  5. HPRT 基因家族的基因重复和失活。  |  Keebaugh, AC., et al. 2007. Genomics. 89: 134-42. PMID: 16928426
  6. 中国接触过 1,3-丁二烯的工人淋巴细胞中的 HPRT 突变。  |  Liu, S., et al. 2008. Environ Health Perspect. 116: 203-8. PMID: 18288319
  7. HPRT及其在癌症中新兴作用的综述。  |  Townsend, MH., et al. 2018. Med Oncol. 35: 89. PMID: 29730818
  8. HPRT1中p.V35M突变引起的HPRT相关性高尿酸血症表现为家族性青少年痛风。  |  Mishima, E., et al. 2020. CEN Case Rep. 9: 210-214. PMID: 32128695
  9. HPRT：基因结构, 表达和突变。  |  Stout, JT. and Caskey, CT. 1985. Annu Rev Genet. 19: 127-48. PMID: 3909940
  10. 人类T淋巴细胞中hprt基因座突变分析。  |  Lambert, B., et al. 1995. Toxicol Lett. 82-83: 323-33. PMID: 8597072
  11. 次黄嘌呤磷酸核糖转移酶（HPRT）基因中的一种无症状种系错义碱基置换，会减少人体中的酶量。  |  Fujimori, S., et al. 1997. Hum Genet. 99: 8-10. PMID: 9003484

订购信息

产品名称产品编号规格价格数量收藏夹

HPRT 抗体 (F-1)

sc-376938
200 µg/ml
$316.00

HPRT 抗体 (F-1) AC

sc-376938 AC
500 µg/ml, 25% agarose
$416.00

HPRT 抗体 (F-1) HRP

sc-376938 HRP
200 µg/ml
$316.00

HPRT 抗体 (F-1) FITC

sc-376938 FITC
200 µg/ml
$330.00

HPRT 抗体 (F-1) PE

sc-376938 PE
200 µg/ml
$343.00

HPRT 抗体 (F-1) Alexa Fluor® 488

sc-376938 AF488
200 µg/ml
$357.00

HPRT 抗体 (F-1) Alexa Fluor® 546

sc-376938 AF546
200 µg/ml
$357.00

HPRT 抗体 (F-1) Alexa Fluor® 594

sc-376938 AF594
200 µg/ml
$357.00

HPRT 抗体 (F-1) Alexa Fluor® 647

sc-376938 AF647
200 µg/ml
$357.00

HPRT 抗体 (F-1) Alexa Fluor® 680

sc-376938 AF680
200 µg/ml
$357.00

HPRT 抗体 (F-1) Alexa Fluor® 790

sc-376938 AF790
200 µg/ml
$357.00

HPRT (F-1) 中和勝肽

sc-376938 P
100 µg/0.5 ml
$68.00

Does it cross-react with mouse?

Asked by: Laura
Thank you for your question. HPRT Antibody (F-1) is recommended for detection of HPRT of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine and porcine.
Answered by: Tech Support Europe
Date published: 2025-04-08

What dilution of sc-376938 did you use for the WB images on top of the website?

Asked by: RStr19
Thank you for your question. The recommended starting dilution for this antibody is 1:100, which is what was used here. Depending on your samples and experimental setup, a dilution range between 1:100-1:1000 is appropriate.
Answered by: Tech Support Europe
Date published: 2024-05-03

What application is the blocking peptide sc-376938 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_376938, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 106ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from works well for WBWorks well on neonatal mouse lungs at concentration of 30ug of protein. Ab dilution will be 1:1000 for overnight @ 4oC.
Date published: 2024-12-10
Rated 4 out of 5 by from Works well as loading controlThe antibody works well as loading control for western blot in human neutrophils (1:500 dilution).
Date published: 2023-12-06
Rated 5 out of 5 by from Klare Banden Im Western BlotSehr guter house-keeping Antikörper für Versuche mit Hypoxie
Date published: 2018-08-30
Rated 5 out of 5 by from great gobit works very well, the band is very clear ,great!
Date published: 2017-11-22
Rated 5 out of 5 by from Produced publishable WB data in mouseThis HPRT monoclonal antibody was referenced in a recent publication for the use in WB on mouse BMMCs
Date published: 2017-02-02
Rated 5 out of 5 by from Good antibody for western in A549 and MIA PaCaGood antibody for western in A549 and MIA PaCa-2 whole cell lysates. - SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-04-30
Rated 5 out of 5 by from StrongStrong, specific band of interest obtained in WB in Hep G2, KNRK, HeLa, A-431 and MCF7 whole cell lysates. -SCBT QC
Date published: 2015-03-31
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_376938, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
HPRT 抗体 (F-1) is rated 4.9 out of 5 by 8.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_376938, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。