Date published: 2025-9-6
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Homer Antibody (D-3) - Western Blotting - Image 381356
Homer 抗体 (D-3): sc-17842. IMR-32 (A), A-431 (B), MIA PaCa-2 (C), AN3 CA (D), NIH/3T3 (E) 和 C2C12 (F) 全细胞裂解液中 Homer 表达的 Western 印迹分析.
Homer 抗体 (D-3): sc-17842. IMR-32 (A), A-431 (B), MIA PaCa-2 (C), AN3 CA (D), NIH/3T3 (E) 和 C2C12 (F) 全细胞裂解液中 Homer 表达的 Western 印迹分析.

Homer 抗体 (D-3): sc-17842

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说明书
  • Homer 抗体 D-3 是小鼠单克隆 IgG1 κ，Homer抗体, 在24篇文献中引用，规格为200 µg/ml
  • 针对human起源的Homer的1-354氨基酸；识别需要氨基酸13-181
  • Homer 抗体 (D-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Homer
  • 抗Homer抗体（D-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Homer (D-3): sc-17842的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Homer 抗体 (D-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Homer 抗体 (D-3) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Homer 抗体包括 Homer-1, Homer-1b, Homer-1c 和 Homer-3

Homer抗体（D-3）是一种小鼠单克隆IgG1 kappa轻链抗体，可特异性识别小鼠、大鼠和人类来源的Homer蛋白，适用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）等应用。荷马蛋白（Homer proteins）也称为Vesl蛋白（VASP/Ena-related gene upregulated during seizure），是一组支架蛋白，通过与第一类代谢型谷氨酸受体（mGluRs）结合，在突触信号传导中发挥关键作用，而mGluRs则参与触发细胞内钙离子释放。Homer蛋白与mGluRs之间的相互作用对于突触可塑性和神经传递至关重要，影响着学习和记忆过程。Homer-1a是一种由神经活动诱导的早期基因产物，与其他恒定表达的Homer蛋白（如Homer-1b、-1c、-2和-3）不同，它缺少螺旋线圈结构域，无法进行多聚化，并调节突触信号通路。通过将mGluRs与细胞内钙通道（如三磷酸肌醇受体（IP3R））连接起来，Homer蛋白有助于调节细胞内钙动态，这对于许多神经元功能至关重要。了解Homer蛋白的功能和调节对于神经疾病和突触功能障碍的研究非常重要。Homer (D-3) 单克隆抗体有未结合和结合两种形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种Alexa Fluor®结合物，可满足不同实验需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Homer 抗体 (D-3) 参考文献:

  1. 小鼠大脑和肌肉组织会持续表达高水平的荷马蛋白。  |  Soloviev, MM., et al. 2000. Eur J Biochem. 267: 634-9. PMID: 10651798
  2. 通过广泛的替代剪接产生的两组结构不同的人类同源物的分子特征。  |  Soloviev, MM., et al. 2000. J Mol Biol. 295: 1185-200. PMID: 10653696
  3. Homer-3 通过其 EVH1 结构域调节 T 细胞中血清反应元件的激活。  |  Ishiguro, K. and Xavier, R. 2004. Blood. 103: 2248-56. PMID: 14645007
  4. Homer：一种选择性结合代谢型谷氨酸受体的蛋白质。  |  Brakeman, PR., et al. 1997. Nature. 386: 284-8. PMID: 9069287
  5. vesl 是一种编码 VASP/Ena 家族相关蛋白的基因，在癫痫发作, 长期延时和突触发生过程中上调。  |  Kato, A., et al. 1997. FEBS Lett. 412: 183-9. PMID: 9257717
  6. 结合代谢型谷氨酸受体的 PDZ 蛋白 Vesl/Homer 家族的新成员。  |  Kato, A., et al. 1998. J Biol Chem. 273: 23969-75. PMID: 9727012
  7. Homer调节1类代谢谷氨酸受体与Homer相关的多价复合体, 突触蛋白的缔合作用。  |  Xiao, B., et al. 1998. Neuron. 21: 707-16. PMID: 9808458
  8. Homer 与一种新型富脯氨酸基团结合，并将第 1 组代谢谷氨酸受体与 IP3 受体连接起来。  |  Tu, JC., et al. 1998. Neuron. 21: 717-26. PMID: 9808459

订购信息

产品名称产品编号规格价格数量收藏夹

Homer 抗体 (D-3)

sc-17842
200 µg/ml
$316.00

Homer (D-3): m-IgG Fc BP-HRP 套装

sc-528333
200 µg Ab; 10 µg BP
$354.00

Homer (D-3): m-IgGκ BP-HRP 套装

sc-520681
200 µg Ab, 40 µg BP
$354.00

Homer (D-3): m-IgG1 BP-HRP 套装

sc-542871
200 µg Ab; 20 µg BP
$354.00

Homer 抗体 (D-3) AC

sc-17842 AC
500 µg/ml, 25% agarose
$416.00

Homer 抗体 (D-3) HRP

sc-17842 HRP
200 µg/ml
$316.00

Homer 抗体 (D-3) FITC

sc-17842 FITC
200 µg/ml
$330.00

Homer 抗体 (D-3) PE

sc-17842 PE
200 µg/ml
$343.00

Homer 抗体 (D-3) Alexa Fluor® 488

sc-17842 AF488
200 µg/ml
$357.00

Homer 抗体 (D-3) Alexa Fluor® 546

sc-17842 AF546
200 µg/ml
$357.00

Homer 抗体 (D-3) Alexa Fluor® 594

sc-17842 AF594
200 µg/ml
$357.00

Homer 抗体 (D-3) Alexa Fluor® 647

sc-17842 AF647
200 µg/ml
$357.00

Homer 抗体 (D-3) Alexa Fluor® 680

sc-17842 AF680
200 µg/ml
$357.00

Homer 抗体 (D-3) Alexa Fluor® 790

sc-17842 AF790
200 µg/ml
$357.00

Hi, I was wondering if I could request a sample of this antibody to test out in our assay? We want to detect synaptic proteins in mouse brain sections by IHC using confocal microscopy.

Asked by: jcook5
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-14

Does this antibody detect Homer1, Homer2, and Homer3 as well?

Asked by: Nikolas
Thank you for your question. This mouse monoclonal antibody, Homer (D-3): sc-17842, is raised against Homer-1 of human origin (UniProt accession number: Q86YM7). The exact epitope recognized by this monoclonal antibody has not been determined, but based on sequence analysis there is approximately a 50% chance that this antibody may also cross-react with Homer-2 and Homer-3. This cross-reactivity has not yet been confirmed experimentally.
Answered by: Technical Support
Date published: 2017-09-11

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-01-27
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Rated 4 out of 5 by from Good Ab in western blotTested by WB on mouse hippocampal lysate (1:2000 dilution): 2 prominent bands (likely Homer 1b and 1c) at the expected MW
Date published: 2024-11-21
Rated 4 out of 5 by from Buon funzionamentoHo testato questo anticorpo su lisati ottenuti da tessuti di topo ed ho ottenuto un ottimo risultato, l'anticorpo da un segnale molto forte che compare all'altezza prevista.
Date published: 2021-02-22
Rated 5 out of 5 by from Western blotWe diluted the antibody 1:500, very clear band was showed in the membrane.
Date published: 2020-11-15
Rated 5 out of 5 by from Great antibody for WBThis antibody is goo for western, i got strong band and clear background.
Date published: 2017-03-23
Rated 5 out of 5 by from Useful for IP/WBThe (D-3) clone has been featured in 6 publications, and is frequently used for IP and WB for Homer in mouse, rat and human samples.
Date published: 2016-12-19
Rated 5 out of 5 by from Good specificity in WB in human transfectedGood specificity in WB in human transfected 293 whole cell lysates and mouse brain tissue extract. -SCBT QC
Date published: 2015-06-13
Rated 5 out of 5 by from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates and rat brain tissue extract. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Positive signal in WB in TE671 and IMRPositive signal in WB in TE671 and IMR-32 whole cell lysates and mouse brain and rat brain tissue extracts. -SCBT QC
Date published: 2015-05-12
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Homer 抗体 (D-3) is rated 4.8 out of 5 by 11.
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