Date published: 2025-9-5

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hnRNP K/J 抗体 (3C2): sc-32307

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说明书
  • hnRNP K/J 抗体 3C2 是小鼠单克隆 IgG2b κ,hnRNP K/J抗体, 在20篇文献中引用,规格为200 µg/ml
  • 针对通过寡核苷酸(dc)亲和色谱纯化的hnRNPs
  • hnRNP K/J 抗体 (3C2) 推荐用于WB, IP, IF, IHC(P) 和 FCM, 检测 mouse, rat, human, Xenopus 和 bacterial 来源的 hnRNP K and hnRNP J
  • 抗hnRNP K/J抗体(3C2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取hnRNP K/J (3C2): sc-32307免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 是hnRNP K/J 抗体 (3C2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与hnRNP K/J 抗体 (3C2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

hnRNP K/J抗体(3C2)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和流式细胞术(FC)检测小鼠、大鼠、人类和非洲爪蟾来源的hnRNP K/J蛋白 M)。hnRNP K/J (3C2) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。异质核糖核蛋白K(hnRNP K)在mRNA代谢中起着至关重要的作用,包括转录、前mRNA处理以及将成熟的mRNA转运到细胞质中进行翻译。hnRNP K主要定位于细胞核,但可以在细胞核和细胞质之间穿梭,这对于调节基因表达至关重要。hnRNP K包含两个K同源(KH)结构域,可促进与各种分子伴侣的相互作用,从而影响多种细胞过程。此外,hnRNP K 具有多个磷酸化位点,包括 Ser 302,这是蛋白激酶 C delta 磷酸化的重要位点。这种翻译后修饰可调节 hnRNP K 结合 DNA 和 RNA 的能力,从而影响其在转录调控和核质穿梭中的作用。hnRNP K 与其他蛋白相互作用的多功能性及其动态定位强调了其在细胞功能和基因表达调控中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP K/J 抗体 (3C2) 参考文献:

  1. 蛋白激酶 Cdelta 与异质核糖核蛋白 K 蛋白的调节性相互作用。  |  Schullery, DS., et al. 1999. J Biol Chem. 274: 15101-9. PMID: 10329716
  2. 核前 mRNA 区隔:将释放的转录本运送到剪接因子库。  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
  3. 在细胞核和细胞质之间穿梭的 hnRNPs 之间的蛋白质相互作用。  |  Kim, JH., et al. 2000. J Mol Biol. 298: 395-405. PMID: 10772858
  4. 生长因子调控异质核糖核蛋白 K 的表达和功能。  |  Mandal, M., et al. 2001. J Biol Chem. 276: 9699-704. PMID: 11121407
  5. 异质核糖核蛋白 K(hnRNP K)在癌症进展中的新作用。  |  Barboro, P., et al. 2014. Cancer Lett. 352: 152-9. PMID: 25016060
  6. LCDR 通过 hnRNP K 稳定的 LAPTM5 转录物调节溶酶体膜的完整性,促进细胞存活。  |  Yang, X., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35091468
  7. 癌症-睾丸lncRNA lnc-CTHCC通过结合hnRNP K和激活YAP1转录促进肝细胞癌变。  |  Xia, A., et al. 2022. Nat Cancer. 3: 203-218. PMID: 35122073
  8. HnRNP K 通过介导转录因子的剪接模式调节炎症基因的表达。  |  Liu, S., et al. 2023. Exp Biol Med (Maywood). 248: 1479-1491. PMID: 35866661
  9. hnRNP K 可诱导 HPV16 致癌基因的表达并促进宫颈癌的发生。  |  Lyu, Y., et al. 2023. J Cancer Res Clin Oncol. 149: 6225-6237. PMID: 36700980
  10. hnRNP A1 蛋白的核定位结构域。  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
  11. 前核糖核酸结合 K 蛋白含有一个新的进化保守基团。  |  Siomi, H., et al. 1993. Nucleic Acids Res. 21: 1193-8. PMID: 8464704
  12. 新型人类 RNA 结合蛋白的鉴定和特性分析。  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP K/J 抗体 (3C2)

sc-32307
200 µg/ml
$316.00

hnRNP K/J (3C2): m-IgG2b BP-HRP 套装

sc-548653
200 µg Ab; 10 µg BP
$354.00

hnRNP K/J 抗体 (3C2) AC

sc-32307 AC
500 µg/ml, 25% agarose
$416.00

hnRNP K/J 抗体 (3C2) HRP

sc-32307 HRP
200 µg/ml
$316.00

hnRNP K/J 抗体 (3C2) FITC

sc-32307 FITC
200 µg/ml
$330.00

hnRNP K/J 抗体 (3C2) PE

sc-32307 PE
200 µg/ml
$343.00

hnRNP K/J 抗体 (3C2) Alexa Fluor® 488

sc-32307 AF488
200 µg/ml
$357.00

hnRNP K/J 抗体 (3C2) Alexa Fluor® 546

sc-32307 AF546
200 µg/ml
$357.00

hnRNP K/J 抗体 (3C2) Alexa Fluor® 594

sc-32307 AF594
200 µg/ml
$357.00

hnRNP K/J 抗体 (3C2) Alexa Fluor® 647

sc-32307 AF647
200 µg/ml
$357.00

hnRNP K/J 抗体 (3C2) Alexa Fluor® 680

sc-32307 AF680
200 µg/ml
$357.00

hnRNP K/J 抗体 (3C2) Alexa Fluor® 790

sc-32307 AF790
200 µg/ml
$357.00

Can I get the epitope sequence or approximate region? That will be great.

Asked by: WEI huaxia
Thank you for your question. This IgG2bκ antibody clone was raised against purified full length human hnRNP K/J. Epitope mapping has not been carried out and thus binding region yet remains uncharacterized.
Answered by: Technical Support Europe
Date published: 2017-12-29

I am using hnRNP K/J (3C2): sc-32307 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works very well for WBworks very well for WB
Date published: 2015-10-20
Rated 4 out of 5 by from Publishable data for WB and CoPublishable data for WB and Co-IP in human epidermoid carcinoma A431 cells. -SCBT Publication Review
Date published: 2015-05-09
Rated 4 out of 5 by from Very robust signal in WB in nonVery robust signal in WB in non-transfected and mouse hnRNP K transfected 293T whole cell lysates and HeLa nuclear extract. -SCBT QC
Date published: 2015-04-28
Rated 4 out of 5 by from Very strong signal in WB in nonVery strong signal in WB in non-transfected and human hnRNP K transfected) 293T whole cell lysates. -SCBT QC
Date published: 2015-03-14
Rated 4 out of 5 by from Published data for IHC (acetone) in XenopusPublished data for IHC (acetone) in Xenopus laevis embryos. -SCBT Publication Review
Date published: 2015-02-05
Rated 4 out of 5 by from Strong band in WB in Sol8Strong band in WB in Sol8, KNRK and HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-14
Rated 4 out of 5 by from Wonderful immunoperoxidase nuclear and cytoplasmicWonderful immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-01-10
Rated 4 out of 5 by from Published data for WB in human 293T cellsPublished data for WB in human 293T cells. -SCBT Publication Review
Date published: 2015-01-05
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hnRNP K/J 抗体 (3C2) is rated 4.1 out of 5 by 9.
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