Date published: 2026-10-19
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hnRNP A1 Antibody (9H10) - Western Blotting - Image 46462
hnRNP A1 抗体 (9H10): sc-56700. 未转染 293T: sc-117752 (A), 转染小鼠 hnRNP A1 的 293T: sc-120851 (B) 和 Jurkat (C) 全细胞裂解液中 hnRNP A1 表达的 Western 印迹分析.
hnRNP A1 抗体 (9H10): sc-56700. 未转染 293T: sc-117752 (A), 转染小鼠 hnRNP A1 的 293T: sc-120851 (B) 和 Jurkat (C) 全细胞裂解液中 hnRNP A1 表达的 Western 印迹分析.

hnRNP A1 抗体 (9H10): sc-56700

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说明书
  • hnRNP A1 抗体 9H10 是小鼠单克隆 IgG2b, 在17篇文献中引用，提供 200 µg/ml
  • 针对全长hnRNP A1，human起源
  • 推荐用于 mouse, rat 和 human 来源的hnRNP A1 WB, IP, IF 和 ELISA检测
  • 看 hnRNP A1 (4B10): sc-32301，可查看有标记的 hnRNP A1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是hnRNP A1 Antibody (9H10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与hnRNP A1 Antibody (9H10) 打包提供（请参阅下面的订购信息）。
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関連項目

额外hnRNP抗体，包括hnRNP A, hnRNP A1, hnRNP A2, hnRNP B, hnRNP B1, hnRNP C1, hnRNP C2, hnRNP E1, hnRNP E2, hnRNP F, hnRNP H, hnRNP H3, hnRNP I, hnRNP J, hnRNP K和hnRNP L

hnRNP A1 抗体（9H10）是一种 IgG2b κ 小鼠单克隆 hnRNP A1 抗体，可通过 WB、IP、IF 和 ELISA 检测小鼠、大鼠和人类的 hnRNP A1。hnRNP A1 抗体（9H10）为非偶联的抗 hnRNP A1 抗体形式。异质核核糖核蛋白（hnRNPs）构成一组多肽，这些多肽有助于 mRNA 转录和前 mRNA 加工，以及成熟 mRNA 向细胞质的转运和翻译。它们还与异质核 RNA（hnRNA）结合，这是由 RNA 聚合酶 II 产生的转录物。目前已知大约有 20 种 hnRNP 蛋白，它们的复合物是剪接体的主要组成部分。大多数 hnRNP 蛋白组分定位于细胞核中，然而有些则在细胞核和细胞质之间穿梭。hnRNPs 的 A/B 亚家族包括 A1、A2/B1、A3 和 A0，在非洲爪蟾中，hnRNP A1、A2 和 A3 在整个发育过程以及成年组织中普遍表达。hnRNP A1 和 A2/B1 通过直接拮抗各种剪接因子的结合以及通过影响前 mRNA 上的剪接位点选择来调节前 mRNA 的加工。hnRNP A0 基因与其他 A/B 家族成员不同，它编码一种低丰度蛋白，与 mRNA 稳定性有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

hnRNP A1 抗体 (9H10) 参考文献:

  1. HnRNP A1/A2蛋白通过上下文依赖性相互作用组装到7SK snRNA上。  |  Luo, L., et al. 2021. J Mol Biol. 433: 166885. PMID: 33684393
  2. 对hnRNP A1功能与功能障碍在神经退行性疾病发病机理中的作用进行全面分析。  |  Clarke, JP., et al. 2021. Front Mol Biosci. 8: 659610. PMID: 33912591
  3. HNRNP A1通过调节VRK1翻译促进肺癌细胞增殖。  |  Ryu, HG., et al. 2021. Int J Mol Sci. 22: PMID: 34071140
  4. hnRNP A1/A2在SMN1/2外显子7剪接中的位置依赖效应。  |  Qiu, J., et al. 2022. Biochim Biophys Acta Gene Regul Mech. 1865: 194875. PMID: 36208849
  5. 全面了解hnRNP A1在癌症中的作用：与非编码RNA结合的新视角。  |  Siculella, L., et al. 2023. Cancer Gene Ther. 30: 394-403. PMID: 36460805
  6. HnRNP A1抑制牙源性分化并抑制牙源性间充质细胞RUNX2外显子5的包含。  |  Zhang, Y., et al. 2023. Front Biosci (Landmark Ed). 28: 139. PMID: 37525910
  7. 在阿尔茨海默氏病模型中，抑制hnRNP A1与HK1 RNA的结合会导致糖酵解功能障碍。  |  Ji, XH., et al. 2023. Front Aging Neurosci. 15: 1218267. PMID: 37744386
  8. microRNA-195-5p/hnRNP A1轴通过调节癌细胞的迁移，促进肝细胞癌的进展。  |  Jung, M., et al. 2023. Biochem Biophys Res Commun. 686: 149183. PMID: 37926044
  9. β-Arrestin2通过促进hnRNP A1介导的PKM2选择性剪接，促进去势抵抗性前列腺癌对多西他赛的耐药性。  |  Zhou, Y., et al. 2023. Discov Oncol. 14: 215. PMID: 38019357
  10. T细胞分化与功能无需hnRNP A1-Serine 199的磷酸化。  |  White, TLA., et al. 2024. Immunohorizons. 8: 136-146. PMID: 38334757

订购信息

产品名称产品编号规格价格数量收藏夹

hnRNP A1 抗体 (9H10)

sc-56700
200 µg/ml
$322.00

hnRNP A1 (9H10): m-IgG Fc BP-HRP 套装

sc-537116
200 µg Ab; 10 µg BP
$361.00

hnRNP A1 (9H10): m-IgGκ BP-HRP 套装

sc-534308
200 µg Ab; 40 µg BP
$361.00

hnRNP A1 (9H10): m-IgG2b BP-HRP 套装

sc-549856
200 µg Ab; 10 µg BP
$361.00

I am using hnRNP A1 (9H10): sc-56700 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by arnoldone from It works perfectly in WBhnRNP A1 Antibody (9H10): sc-56700. Western blot analysis of hnRNP A1 expression in human Neural Progenitor cells (hNPCs) after nuclear (N) and cytoplasmic (C) fractionation.
Date published: 2021-09-03
Rated 4 out of 5 by STneuro from GoodA band of predicted size was detected in WB (HEK293 and mouse brain lysate).
Date published: 2020-06-26
Rated 5 out of 5 by Bertrand from Works for WB of endogenousDetects a band of the correct size at 1/200 on HEK293T cell lysate
Date published: 2017-09-13
Rated 5 out of 5 by TO from Very robust signal in WB in nonVery robust signal in WB in non-transfected 293T and human hnRNP A1 transfected 293T whole cell lysates and HeLa nuclear extract. -SCBT QC
Date published: 2015-05-14
Rated 5 out of 5 by HF from Published WB and CoIP data for use in humanPublished WB and CoIP data for use in human epidermoid carcinoma A431 cells. -SCBT Publication Review
Date published: 2015-05-13
Rated 5 out of 5 by FL from StrongStrong, clear signal in WB in mouse hnRNP A1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-05-13
Rated 5 out of 5 by JT from Published data for WB in HeLa cellsPublished data for WB in HeLa cells. -SCBT Publication Review
Date published: 2015-03-29
Rated 5 out of 5 by CJ from Very strong band in WB in HeLaVery strong band in WB in HeLa, MCF7, K-562 and SW480 nuclear extracts and Jurkat and HeLa whole cell lysates. -SCBT QC
Date published: 2014-09-25
  • y_2026, m_10, d_19, h_5
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hnRNP A1 抗体 (9H10) is rated 4.9 out of 5 by 8.
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