HMG-20A CRISPR/Cas9 KO Plasmid (h): sc-407302
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HMG-20A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the HMG-20A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HMG-20A Antibody (D-5): sc-393028
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HMG-20A CRISPR/Cas9 KO Plasmid (h) | sc-407302 | 20 µg | $397.00 |
Overview
HMG20A encodes HMG-20A, a high mobility group–like chromatin-associated factor that contributes to transcriptional regulation by influencing nucleosome organization and access of regulatory complexes to DNA. It has been implicated in epigenetic control of lineage programs, including neurodevelopmental gene expression, through interactions with chromatin remodeling and histone-modifying machinery. By shaping promoter and enhancer activity, HMG-20A can affect cell-cycle control, differentiation, and maintenance of cell identity. Altered regulation of HMG20A-linked chromatin states is relevant to studies of neurological phenotypes and cancer-associated transcriptional dysregulation.
HMG-20A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the HMG20A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the HMG20A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the HMG20A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish HMG-20A protein expression.
This CRISPR knockout system enables efficient generation of HMG20A-deficient cell models for investigation of HMG-20A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting HMG20A exon(s) critical for HMG-20A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple HMG20A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by HMG-20A CRISPR/Cas9 KO Plasmid (h) and HMG-20A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the HMG20A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by HMG-20A HDR Plasmid (h) and HMG-20A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by HMG20A homology arms to support homology-directed repair at defined HMG20A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.