Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
HIPK2 Double Nickase Plasmid (h): sc-402003-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HIPK2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HIPK2 Double Nickase Plasmid (h) and HIPK2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HIPK2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HIPK2 Antibody (F-189): sc-100383
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HIPK2 Double Nickase Plasmid (h) | sc-402003-NIC | 20 µg | $410.00 | |||
HIPK2 Double Nickase Plasmid (h2) | sc-402003-NIC-2 | 20 µg | $410.00 |
Human HIPK2 (homeodomain-interacting protein kinase 2) is a serine/threonine kinase that integrates stress and developmental signals by phosphorylating transcriptional regulators, including p53, and modulating chromatin-associated complexes. It participates in DNA damage responses, apoptosis and senescence programs, and transcriptional control through pathways linked to ATM/ATR signaling, Wnt/β-catenin regulation, and TGF-β–responsive gene expression. HIPK2 also interfaces with ubiquitin-dependent turnover and nuclear body dynamics, shaping context-dependent gene expression outcomes. Dysregulated HIPK2 activity and expression have been associated with altered checkpoint control, aberrant survival signaling, and tumor biology, making it a relevant node for mechanistic studies of genome stability and stress-adaptive transcription.
HIPK2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HIPK2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HIPK2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HIPK2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HIPK2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.