Date published: 2025-9-7

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GRASP65 抗体 (D-12): sc-374423

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说明书
  • GRASP65 抗体 D-12 是小鼠单克隆 IgG1 κ,GRASP65抗体, 在8篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的GRASP65的C-terminus附近的氨基酸347-385之间
  • GRASP65 抗体 (D-12) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 GRASP65
  • 抗GRASP65抗体(D-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GRASP65 (D-12): sc-374423免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是GRASP65 Antibody (D-12) for WB applications. 的首选辅助检测试剂,这些试剂现与GRASP65 Antibody (D-12) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

GRASP65抗体(D-12)是一种IgG1 κ型小鼠单克隆GRASP65抗体(也称为GRASP65抗体),它能够通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测到来自小鼠、大鼠和人类的GRASP65蛋白。GRASP65抗体(D-12)既有非偶联的抗GRASP65抗体形式,也有多种偶联形式的抗GRASP65抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。高尔基体是一个高度复杂的细胞器,由从内质网到细胞表面的分泌途径上的一系列扁平膜囊组成。其结构由外骨骼或高尔基体基质维持,该基质由一组卷曲螺旋蛋白、高尔基体蛋白和其他周边膜成分如GRASP55和GRASP65构成。GRASP55(高尔基体重组堆叠蛋白或p59)是高尔基体堆叠机制的一个组成部分。GRASP55与GRASP65高度同源,并含有两个PDZ结构域。GRASP55经过肉豆蔻酰化和棕榈酰化。与GRASP65不同,GRASP55与囊泡对接蛋白GM130没有可检测的结合,并且位于高尔基体中间部分而不是顺式高尔基体。GRASP55和GRASP65都参与高尔基体扁平膜囊的堆叠。新型卷曲螺旋蛋白golgin 45与GRASP55和Rab 2的GTP形式相互作用,这表明GRASP55和golgin 45在高尔基体中间部分形成了一个Rab 2效应复合物,这对正常的蛋白质运输和高尔基体结构至关重要。ERK2直接磷酸化GRASP55,而GRASP55在有丝分裂细胞中被磷酸化,这表明有丝分裂期间,有丝分裂原激活的蛋白激酶激酶(MKK)/ERK通路磷酸化了高尔基体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GRASP65 抗体 (D-12) 参考文献:

  1. GRASP55,在无细胞系统中参与高尔基空腔堆积的第二种哺乳动物 GRASP 蛋白。  |  Shorter, J., et al. 1999. EMBO J. 18: 4949-60. PMID: 10487747
  2. 跨膜转化生长因子-α与含 PDZ 结构域的高尔基体膜缔合作用蛋白 p59/GRASP55 连接。  |  Kuo, A., et al. 2000. EMBO J. 19: 6427-39. PMID: 11101516
  3. 有丝分裂活化蛋白激酶 ERK2 对高尔基体重组堆积蛋白 55 的有丝分裂磷酸化。  |  Jesch, SA., et al. 2001. Mol Biol Cell. 12: 1811-7. PMID: 11408587
  4. 连接高尔基体结构与膜交通的 GRASP55-rab2 效应复合物  |  Short, B., et al. 2001. J Cell Biol. 155: 877-83. PMID: 11739401
  5. 高尔基体基质蛋白与 p24 货物受体相互作用,帮助它们有效地保留在高尔基体中。  |  Barr, FA., et al. 2001. J Cell Biol. 155: 885-91. PMID: 11739402

订购信息

产品名称产品编号规格价格数量收藏夹

GRASP65 抗体 (D-12)

sc-374423
200 µg/ml
$316.00

GRASP65 (D-12): m-IgG Fc BP-HRP 套装

sc-527462
200 µg Ab; 10 µg BP
$354.00

GRASP65 (D-12): m-IgG1 BP-HRP 套装

sc-532835
200 µg Ab; 20 µg BP
$354.00

GRASP65 抗体 (D-12) AC

sc-374423 AC
500 µg/ml, 25% agarose
$416.00

GRASP65 抗体 (D-12) HRP

sc-374423 HRP
200 µg/ml
$316.00

GRASP65 抗体 (D-12) FITC

sc-374423 FITC
200 µg/ml
$330.00

GRASP65 抗体 (D-12) PE

sc-374423 PE
200 µg/ml
$343.00

GRASP65 抗体 (D-12) Alexa Fluor® 488

sc-374423 AF488
200 µg/ml
$357.00

GRASP65 抗体 (D-12) Alexa Fluor® 546

sc-374423 AF546
200 µg/ml
$357.00

GRASP65 抗体 (D-12) Alexa Fluor® 594

sc-374423 AF594
200 µg/ml
$357.00

GRASP65 抗体 (D-12) Alexa Fluor® 647

sc-374423 AF647
200 µg/ml
$357.00

GRASP65 抗体 (D-12) Alexa Fluor® 680

sc-374423 AF680
200 µg/ml
$357.00

GRASP65 抗体 (D-12) Alexa Fluor® 790

sc-374423 AF790
200 µg/ml
$357.00

GRASP65 (D-12) 中和勝肽

sc-374423 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-374423 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from great antibodyI tested this antibody in mouse and human lung cancer cells and it works very good.
Date published: 2017-10-03
Rated 1 out of 5 by from No IF signals in mice/rat mandible ameloblastsThis antibody doesn't show bindings on PFA fixed and paraffin embedded sections from mice/rat mandible ameloblasts.
Date published: 2017-07-06
Rated 3 out of 5 by from IF yes, WB noWorks fine in IF, but is not suitable for WB on human lung fibroblasts which do highly express GRASP65. Empty blots, after very long expsoure some background signals appear.
Date published: 2017-06-12
Rated 5 out of 5 by from Good specificity in WB in HepG2Good specificity in WB in HepG2, HeLa, MCF7, OVCAR-3 and A549 whole cell lysates. -SCBT QC
Date published: 2015-07-03
Rated 5 out of 5 by from Strong band in WB in human transfected 293TStrong band in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from Excellent immunofluorescence Golgi apparatusExcellent immunofluorescence Golgi apparatus staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-08
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GRASP65 抗体 (D-12) is rated 4.0 out of 5 by 6.
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