Date published: 2025-9-8
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GPNMB Antibody (D-9) - Western Blotting - Image 354277
GPNMB 抗体 (D-9): sc-271415. SK-MEL-28 (A), C32 (B) 和 A-375 (C) 全细胞裂解液中 GPNMB 表达的 Western 印迹分析.
GPNMB 抗体 (D-9): sc-271415. SK-MEL-28 (A), C32 (B) 和 A-375 (C) 全细胞裂解液中 GPNMB 表达的 Western 印迹分析.

GPNMB 抗体 (D-9): sc-271415

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寫評論提問

说明书
  • GPNMB 抗体 D-9 是小鼠单克隆 IgG1 κ，GPNMB抗体, 在4篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列38-158位于human物种的GPNMB的N-terminus附近
  • GPNMB 抗体 (D-9) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 GPNMB isoforms 1 and 2
  • 抗GPNMB抗体（D-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GPNMB (D-9): sc-271415的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和m-IgGκ BP-HRP是GPNMB Antibody (D-9) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与GPNMB Antibody (D-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

GPNMB抗体（D-9）是一种IgG1 κ小鼠单克隆GPNMB抗体（也称为GPNMB抗体），可通过WB、IP、IF和ELISA检测到人源的GPNMB蛋白。GPNMB抗体（D-9）既有非偶联形式的抗GPNMB抗体，也有多种偶联形式的抗GPNMB抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。跨膜糖蛋白NMB（GPNMB），也被称为造血生长因子诱导性神经激肽-1（HGFIN），是一种单次跨膜I型膜蛋白。GPNMB属于Pmel-17/NMB蛋白家族，作为一种黑色素生成酶。GPNMB的表达不仅限于黑色素细胞系细胞，在低转移性黑色素瘤细胞系中表达最高。而在高转移性黑色素瘤细胞系中无表达。GPNMB可能在淋巴造血干细胞的成熟过程中发挥重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GPNMB 抗体 (D-9) 参考文献:

  1. 编码黑体蛋白的基因突变会导致 DBA/2J 小鼠出现色素性青光眼。  |  Anderson, MG., et al. 2002. Nat Genet. 30: 81-5. PMID: 11743578
  2. 在成骨细胞中表达的新型 cDNA--骨活素的克隆和特征描述。  |  Safadi, FF., et al. 2001. J Cell Biochem. 84: 12-26. PMID: 11746512
  3. 造血生长因子诱导的神经激肽-1 型：一种类似于神经激肽 1 的跨膜蛋白与 P 物质相互作用。  |  Bandari, PS., et al. 2003. Regul Pept. 111: 169-78. PMID: 12609765
  4. 小鼠糖蛋白（跨膜）nmb（Gpnmb）基因的 mRNA 表达与发育中的视网膜色素上皮细胞和虹膜有关。  |  Bächner, D., et al. 2002. Brain Res Gene Expr Patterns. 1: 159-65. PMID: 12638126
  5. 以缺乏胆碱和 L- 氨基酸的饮食喂养的大鼠在肝硬化发展过程中表达的 Osteoactivin 可加速肝癌细胞的移动。  |  Onaga, M., et al. 2003. J Hepatol. 39: 779-85. PMID: 14568261
  6. HGFIN基因5'侧翼区域的克隆和表征表明，p53和细胞因子介导的转录因子之间存在合作作用：与细胞周期调控有关。  |  Metz, RL., et al. 2005. Cell Cycle. 4: 315-22. PMID: 15684612
  7. 大鼠, 人类肝脏和离体大鼠肝细胞中骨活素的表达。  |  Haralanova-Ilieva, B., et al. 2005. J Hepatol. 42: 565-72. PMID: 15763343

订购信息

产品名称产品编号规格价格数量收藏夹

GPNMB 抗体 (D-9)

sc-271415
200 µg/ml
$316.00

GPNMB (D-9): m-IgG Fc BP-HRP 套装

sc-529179
200 µg Ab; 10 µg BP
$354.00

GPNMB (D-9): m-IgGκ BP-HRP 套装

sc-521937
200 µg Ab, 40 µg BP
$354.00

GPNMB 抗体 (D-9) AC

sc-271415 AC
500 µg/ml, 25% agarose
$416.00

GPNMB 抗体 (D-9) HRP

sc-271415 HRP
200 µg/ml
$316.00

GPNMB 抗体 (D-9) FITC

sc-271415 FITC
200 µg/ml
$330.00

GPNMB 抗体 (D-9) PE

sc-271415 PE
200 µg/ml
$343.00

GPNMB 抗体 (D-9) Alexa Fluor® 488

sc-271415 AF488
200 µg/ml
$357.00

GPNMB 抗体 (D-9) Alexa Fluor® 546

sc-271415 AF546
200 µg/ml
$357.00

GPNMB 抗体 (D-9) Alexa Fluor® 594

sc-271415 AF594
200 µg/ml
$357.00

GPNMB 抗体 (D-9) Alexa Fluor® 647

sc-271415 AF647
200 µg/ml
$357.00

GPNMB 抗体 (D-9) Alexa Fluor® 680

sc-271415 AF680
200 µg/ml
$357.00

GPNMB 抗体 (D-9) Alexa Fluor® 790

sc-271415 AF790
200 µg/ml
$357.00

Whether sc-271415 (gpnmb) can be used in murine tumor cells?

Asked by: Anonymous
Thank you for your question.GPNMB Antibody (D-9) is recommended for detection of human origin.Not recommended for murine.Thank you.
Answered by: Jiajun Z
Date published: 2023-03-16

What is the recommended fixation method for immunofluorescence staining with GPNMB (D-9): sc-271415 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great antibodyWe observed evident and clean bands at the expected molecular weight when testing MCF10A immortalized and MDA-MB-231 tumoral cell lines samples, by immunoblotting. When using this antibody for IF of MDA-MB-231, we observed the expected cytoplasmic localization of GPNMB. Lastly, we successufuly used this antibody for the detection of GPNMB in both normal and tumor breast samples (human) by IHC, even though this antibody seems not to be yet validated for using in such technique.
Date published: 2018-12-02
Rated 5 out of 5 by from GPNMB (D-9)Exelent antibody for western blot. Detected TS at expected size in 15 ug total proteine lysate, wet transfer one hour at 450 mAmps on PVDF membrane, overnight blocking with 5% nonfat dry milk in PBS, one hour incubation at RT with 1:500 anti TS in 5% PBS with 0,2%Tween followed by one hour incubation with 1:10.000 anti mouse HRP conjugated antibody and detection with ECL prime substrate followed by short film exposure (under 1 minute) and automatic processing.
Date published: 2017-09-25
Rated 5 out of 5 by from Nice IF staining in HeLa cellsWe used this antibody for Immunoflourescence staining of GPNMB in HeLa cells. We were able to get a nice signal and resulting images from the staining.
Date published: 2017-02-09
Rated 5 out of 5 by from WB correct mw sized bandDistinct band observed by WB on 10 ug MDA-MB-231T whole cell lysates at 1:1000 dilution.
Date published: 2016-12-12
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmicProduced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-14
Rated 5 out of 5 by from Great Western Blot data of GPNMB expression in SKGreat Western Blot data of GPNMB expression in SK-MEL-28 whole cell lysate. -SCBT QC
Date published: 2013-11-12
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GPNMB 抗体 (D-9) is rated 5.0 out of 5 by 6.
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