Date published: 2025-9-6

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GnRH I 抗体 (HU11B): sc-32292

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说明书
  • GnRH I抗体(HU11B)是小鼠单克隆IgG1 κ, 在28篇文献中引用,规格为200 µg/ml
  • 针对代表rat起源的全长GnRH I十肽的合成肽
  • 抗-GnRH I 抗体 (HU11B) 推荐用于 IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 GnRH I
  • GnRH I 抗体 (HU11B) 可偶联 琼脂糖 用于 IP; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素(sc-32292 B, 200 µg/ml)偶联抗体,可用于WB、IHC(P)和ELISA实验
  • 关于如何获取GnRH I (HU11B): sc-32292免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是GnRH I Antibody (HU11B) for IHC(P) applications. 的首选辅助检测试剂,这些试剂现与GnRH I Antibody (HU11B) 打包提供(请参阅下面的订购信息)。

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関連項目

GnRH I抗体(HU11B)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类样本中的GnRH I蛋白。GnRH I (HU11B) 抗体有非结合型和结合型两种,分别与琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 染料结合。促性腺激素释放激素(GnRH)是一种十肽,在调节下丘脑-垂体-性腺轴方面发挥着关键作用,该轴对于人类生殖至关重要。GnRH由下丘脑神经元合成,并以脉冲形式释放到中脑的毛细血管丛中,刺激前垂体促性腺细胞分泌黄体生成素和促卵泡激素。对GnRH释放的精确调节可确保生殖功能所需的促性腺激素的适当时间和水平。GnRH I在人类精子的顶体区域和垂体前叶组织中表达,突出了其在生殖生理学和潜在癌症生物学中的重要性。GnRH与垂体中特定G蛋白偶联受体的相互作用使得抗GnRH I抗体(HU11B)对于研究生殖健康和相关疾病具有价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GnRH I 抗体 (HU11B) 参考文献:

  1. 促性腺激素释放激素激动剂能够诱导黄体中基质金属蛋白酶(MMP)-2 和膜 1 型-MMP 的表达增加,并导致大鼠黄体结构性溶解。  |  Goto, T., et al. 1999. J Endocrinol. 161: 393-402. PMID: 10333542
  2. 人类精子, 垂体和癌细胞中促性腺激素释放激素受体的免疫鉴定。  |  Lee, CY., et al. 2000. Am J Reprod Immunol. 44: 170-7. PMID: 11028904
  3. 促性腺激素释放激素刺激人前列腺癌细胞株 TSU-Pr1 增殖。  |  Enomoto, M., et al. 2004. Life Sci. 74: 3141-52. PMID: 15081579
  4. 人类 GnRH 受体的 Pro7.33(303)调节哺乳动物 GnRH 的选择性结合。  |  Fromme, BJ., et al. 2004. Mol Cell Endocrinol. 219: 47-59. PMID: 15149726
  5. 促性腺激素释放激素激动剂抑制卵巢囊性子宫内膜异位症雌酮硫酸酯酶的表达  |  Maitoko, K. and Sasaki, H. 2004. Fertil Steril. 82: 322-6. PMID: 15302278
  6. 促性腺激素释放激素(GnRH)拮抗剂通过激活 I 型 GnRH 受体的 Galphai 偶联状态,促进外周生殖肿瘤细胞的促凋亡信号传导。  |  Maudsley, S., et al. 2004. Cancer Res. 64: 7533-44. PMID: 15492280
  7. 人类促性腺激素释放激素(GnRH)-I、GnRH-II及其受体的分子生物学。  |  Cheng, CK. and Leung, PC. 2005. Endocr Rev. 26: 283-306. PMID: 15561800
  8. GnRH I和II通过JNK途径上调人滋养细胞MMP-26的表达。  |  Liu, J., et al. 2010. Reprod Biol Endocrinol. 8: 5. PMID: 20074375
  9. 啮齿动物模型衰老过程中多胺的表达及其与下丘脑GnRH-I的关系。  |  Mate, N., et al. 2022. Amino Acids. 54: 1135-1154. PMID: 35286462
  10. 人类促黄体生成素释放激素前体 cDNA 的特征。  |  Seeburg, PH. and Adelman, JP. Nature. 311: 666-8. PMID: 6090951
  11. 通过表达人类受体的剪接变体抑制促性腺激素释放激素受体的信号传导。  |  Grosse, R., et al. 1997. Mol Endocrinol. 11: 1305-18. PMID: 9259321
  12. 人类促性腺激素释放激素的第二个基因。  |  White, RB., et al. 1998. Proc Natl Acad Sci U S A. 95: 305-9. PMID: 9419371

订购信息

产品名称产品编号规格价格数量收藏夹

GnRH I 抗体 (HU11B)

sc-32292
200 µg/ml
$316.00

GnRH I (HU11B): m-IgG Fc BP-HRP 套装

sc-526674
200 µg Ab; 10 µg BP
$354.00

GnRH I (HU11B): m-IgG1 BP-HRP 套装

sc-532047
200 µg Ab; 20 µg BP
$354.00

GnRH I 抗体 (HU11B) AC

sc-32292 AC
500 µg/ml, 25% agarose
$416.00

GnRH I 抗体 (HU11B) FITC

sc-32292 FITC
200 µg/ml
$330.00

GnRH I 抗体 (HU11B) PE

sc-32292 PE
200 µg/ml
$343.00

GnRH I 抗体 (HU11B) Alexa Fluor® 488

sc-32292 AF488
200 µg/ml
$357.00

GnRH I 抗体 (HU11B) Alexa Fluor® 546

sc-32292 AF546
200 µg/ml
$357.00

GnRH I 抗体 (HU11B) Alexa Fluor® 594

sc-32292 AF594
200 µg/ml
$357.00

GnRH I 抗体 (HU11B) Alexa Fluor® 647

sc-32292 AF647
200 µg/ml
$357.00

GnRH I 抗体 (HU11B) Alexa Fluor® 680

sc-32292 AF680
200 µg/ml
$357.00

GnRH I 抗体 (HU11B) Alexa Fluor® 790

sc-32292 AF790
200 µg/ml
$357.00

GnRH I 抗体 (HU11B) B

sc-32292 B
200 µg/ml
$320.00

您好,请问GNRH单克隆抗体能否用于猪源蛋白检测?

Asked by: yyu123
感谢您的提问。sc-32292 推荐检测大鼠,小鼠,人来源的GnRH I 蛋白。您可以拨打电话:021-60936350,发送邮件到asia@scbio.cn,或者我们官网上的在线聊天,联系我们的亚洲技术支持团队,了解更多信息。 Thank you for your question. sc-32292 is recommended for detecting GnRH I of mouse, rat and human origin. Please contact our Technical Service Department for more information.Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Sen Li
Date published: 2024-01-25

Which secondary antibody would you recommend for this primary antibody?

Asked by: ARISTO
Thank you for your question. You can find our secondary antibodies and binding proteins under this link: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4 Depending on the assay you are planning you could choose a kappa-specific HRP secondary (sc-516102), AF488 secondary (sc-516176) or other conjugate from the list.
Answered by: Tech Support Europe
Date published: 2020-09-29

I'm asking about GnRH I Antibody (HU11B): sc-32292 . Can we use this antibody for a frozen rat brain slices with immunflourescence method.Can you recommend for secondary antibody using with this primer antibody.

Asked by: ZERCAN
Thank you for your question. Yes, you can use this antibody for immunflourescence on frozen rat samples. You can use any anti-mouse IgG secondary antibody, including our suggest mouse IgG binding proteins. Find them here: https://www.scbt.com/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Tech Service
Date published: 2020-01-14

Dear admin, I´m studying on RFRP-3 and GNRH neurons in rat frozen brain tissue with immunfluoresence method. Have you got antibody for this research.

Asked by: ZERCAN
Thank you for your question. Unfortunately we do not offer a RFRP-3 antibody at this time. You can find our available GNRH antibodies here: https://bit.ly/3ag0MT0.
Answered by: Tech Service
Date published: 2020-01-13

Do you have any data demonstrating absence of cross-reactivity with GnRHII in human samples? 

Asked by: NDSH
Thank you for your question. Based on in house testing and sequence homology, this antibody is specific for GnRH I and will not detect GnRH II. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-08-14

I would like to use this monoclonal antibody, GnRH I (HU11B): sc-32292, to stain paraffin embedded tissue sections. Do you recommend antigen retrieval, and if so, which kind?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2017-02-01
  • y_2025, m_8, d_28, h_5CST
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Rated 5 out of 5 by from Antibody worked well in IP stainWe bought this antibody for a IP staining experiment and got a great image
Date published: 2016-12-27
Rated 4 out of 5 by from Publishable data in IHC using human B4 cellsPublishable data in IHC using human B4 cells. -SCBT Publication Review
Date published: 2015-07-13
Rated 4 out of 5 by from Publishable data in WB using human FNCPublishable data in WB using human FNC-B4 cells. -SCBT Publication Review
Date published: 2015-03-10
Rated 4 out of 5 by from Publishable data in IHC(P) using human BreastPublishable data in IHC(P) using human Breast cancer tissue -SCBT Publication Review
Date published: 2015-02-22
Rated 4 out of 5 by from Produced clear immunoperoxidase cytoplasmicProduced clear immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC
Date published: 2015-02-04
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GnRH I 抗体 (HU11B) is rated 4.2 out of 5 by 5.
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