Date published: 2025-9-5
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Glycophorin A Antibody (R10) - Western Blotting - Image 14624
Glycophorin A 抗体 (R10): sc-53905. K-562 (A) 和人 PBL (B) 全细胞裂解液中 Glycophorin A 表达的 Western 印迹分析.
Glycophorin A 抗体 (R10): sc-53905. K-562 (A) 和人 PBL (B) 全细胞裂解液中 Glycophorin A 表达的 Western 印迹分析.

Glycophorin A 抗体 (R10): sc-53905

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说明书
  • Glycophorin A 抗体 R10 是小鼠单克隆 IgG1 κ，Glycophorin A抗体, 在3篇文献中引用，规格为200 µg/ml
  • 针对Abelson鼠白血病病毒诱导的human前B肿瘤细胞
  • Glycophorin A 抗体 (R10) 推荐用于WB, IP, IF, IHC(P) 和 FCM，检测human 来源的 Glycophorin A
  • 抗Glycophorin A抗体（R10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Glycophorin A (R10): sc-53905的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Glycophorin A 抗体 (R10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Glycophorin A 抗体 (R10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Glycophorin 抗体 包括 Glycophorin A, Glycophorin B, Glycophorin C和Glycophorin D

Glycophorin A 抗体 (R10) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF)、石蜡包埋切片免疫组织化学 (IHCP) 和流式细胞术 (FCM) 检测人源 Glycophorin A。抗甘球蛋白 A 抗体 (R10) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Glycophorin A 是位于人类红细胞表面的一种重要的ialoglycoprotein，在维持红细胞结构完整性和促进免疫系统相互作用方面发挥着重要作用。糖蛋白 A 跨膜一次，向细胞外环境呈现氨基末端，对血型抗原性功能至关重要。糖卟啉表面抗原（包括糖卟啉 A、B 和 C）的遗传多样性决定了血型表型，因此这些蛋白质对输血兼容性和了解溶血性疾病至关重要。Glycophorin A 基因位于染色体 4q31.21，由七个外显子组成，与 Glycophorin B 具有高度同源性，编码 91 个氨基酸的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Glycophorin A 抗体 (R10) 参考文献:

  1. 对石蜡包埋骨髓切片中的红细胞前体进行免疫组化鉴定：谱蛋白是优于糖蛋白的标记物。  |  Sadahira, Y., et al. 1999. J Clin Pathol. 52: 919-21. PMID: 10711257
  2. 体内检测糖蛋白-A 及其突变体在膜内的异性结合。  |  Gerber, D. and Shai, Y. 2001. J Biol Chem. 276: 31229-32. PMID: 11402026
  3. 人类糖蛋白 A 的不同区域可增强人类红细胞阴离子交换子（带 3；AE1）的运输功能和表面贩运。  |  Young, MT. and Tanner, MJ. 2003. J Biol Chem. 278: 32954-61. PMID: 12813056
  4. 糖蛋白A是红细胞膜上O-型连接抗原表达的必要条件。  |  Arimitsu, N., et al. 2003. Genes Cells. 8: 769-77. PMID: 12940824
  5. 缺乏糖蛋白 A 的人类红细胞中 3 号带（AE1，SLC4A1）的结构和阴离子转运特性发生改变。  |  Bruce, LJ., et al. 2004. J Biol Chem. 279: 2414-20. PMID: 14604989
  6. 糖蛋白 A 跨膜同源二聚体对 D-氨基酸修饰的结构适应。  |  Gerber, D., et al. 2004. J Mol Biol. 339: 243-50. PMID: 15123435
  7. 螺旋-螺旋界面上的复杂相互作用稳定了糖蛋白 A 跨膜二聚体。  |  Doura, AK. and Fleming, KG. 2004. J Mol Biol. 343: 1487-97. PMID: 15491626
  8. 小鼠NXPE2和小鼠糖蛋白A的共转染赋予了对Ter-119的反应性。  |  Keele, GR., et al. 2024. Haematologica. 109: 3755-3759. PMID: 39021224
  9. 糖蛋白 A 是急性白血病早期红细胞分化的细胞表面标志物。  |  Andersson, LC., et al. 1979. Int J Cancer. 24: 717-20. PMID: 397196
  10. Glycophorin A 在恶性造血中的表达。  |  Liszka, K., et al. 1983. Am J Hematol. 15: 219-26. PMID: 6638008
  11. 人类红细胞祖细胞的细胞表面抗原表达：红细胞和巨核细胞标记物。  |  Nakahata, T. and Okumura, N. 1994. Leuk Lymphoma. 13: 401-9. PMID: 8069185
  12. 通过糖蛋白A的免疫学分析区分瘀伤和皮肤腐败变色。  |  Kibayashi, K., et al. 1993. Forensic Sci Int. 61: 111-7. PMID: 8307520

订购信息

产品名称产品编号规格价格数量收藏夹

Glycophorin A 抗体 (R10)

sc-53905
200 µg/ml
$316.00

Glycophorin A (R10): m-IgG Fc BP-HRP 套装

sc-528587
200 µg Ab; 10 µg BP
$354.00

Glycophorin A (R10): m-IgGκ BP-HRP 套装

sc-520999
200 µg Ab, 40 µg BP
$354.00

Glycophorin A (R10): m-IgG1 BP-HRP 套装

sc-543011
200 µg Ab; 20 µg BP
$354.00

Glycophorin A 抗体 (R10) AC

sc-53905 AC
500 µg/ml, 25% agarose
$416.00

Glycophorin A 抗体 (R10) HRP

sc-53905 HRP
200 µg/ml
$316.00

Glycophorin A 抗体 (R10) FITC

sc-53905 FITC
200 µg/ml
$330.00

Glycophorin A 抗体 (R10) PE

sc-53905 PE
200 µg/ml
$343.00

Glycophorin A 抗体 (R10) Alexa Fluor® 488

sc-53905 AF488
200 µg/ml
$357.00

Glycophorin A 抗体 (R10) Alexa Fluor® 546

sc-53905 AF546
200 µg/ml
$357.00

Glycophorin A 抗体 (R10) Alexa Fluor® 594

sc-53905 AF594
200 µg/ml
$357.00

Glycophorin A 抗体 (R10) Alexa Fluor® 647

sc-53905 AF647
200 µg/ml
$357.00

Glycophorin A 抗体 (R10) Alexa Fluor® 680

sc-53905 AF680
200 µg/ml
$357.00

Glycophorin A 抗体 (R10) Alexa Fluor® 790

sc-53905 AF790
200 µg/ml
$357.00

Could you please share the protocol of the IHC staining of the second image that you posted? My humanized mice samples consist of both human and mouse rbc, will this cause any unspecific binding to mouse rbc?

Asked by: Hooi Linn
Thank you for your question. A link to our recommended protocol that was used for IHC staining with this Glycophorin A Antibody (R10): sc-53905 is provided below. This antibody is recommended for detection of Glycophorin A of human origin. We do not expect the antibody to cross-react with Glycophorin A of mouse origin, however we have not experimentally confirmed this lack of species reactivity. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-05-03

Can Glycophorin A (R10): sc-53905 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jerojero
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Glycophorin A (R10): sc-53905 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-04-28
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Rated 3 out of 5 by from Positive Results in WBWe tested this antibody in WB against red blood cell lysate and we saw a strong and clear signal at 37 KDa. Otherwise, in more diluted samples the antibody didn't work.
Date published: 2022-06-21
Rated 2 out of 5 by from WBWe saw band for Glycopurin A (R10) in the WB of mouse lung primary fibroblast, but it was in the wrong fraction. Instead of membrane fraction, the band was in cytoplasmic fraction. We had negative and positive control for the fraction extraction. The size of the band also was not true, it was about 50 KD. We used anti-mouse IgG1.
Date published: 2018-03-28
Rated 4 out of 5 by from it worked.two month ago, i applied for this antibody and used it to detect the protein in Red blood cell sample, in generally, it worked, but instability.
Date published: 2017-03-04
Rated 5 out of 5 by from Reliable Antibody Great for IHCThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and Flow Cytometry. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-23
Rated 5 out of 5 by from Excellent immunoperoxidase membrane stainingExcellent immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human blood vessel tissue. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from Nice bands seen in WB using KNice bands seen in WB using K-562 and human PBL whole cell lysates. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Excellent FCM data of human red blood cellsExcellent FCM data of human red blood cells stained with Glycophorin A -SCBT QC
Date published: 2014-06-09
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Glycophorin A 抗体 (R10) is rated 4.0 out of 5 by 7.
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