Giemsa Stain (CAS 51811-82-6)

用法 :

Technique used to uniformly stain chromosomes that will leave centromeres constricted:



Method 1

materials:

4 mL Giemsa; 96 mL pH 6.8 phosphate buffer



procedure:

1.Place slides in a Coplin jar or staining dish.

2.Prepare the working stain and pour it over the slides.
Stain for 7 minutes.

3.Rinse slides in two changes of distilled water.

4.Air dry slides; mount them with a cover slip if desired. (If sequential banding procedures are to follow, coverslipping is not recommended.)



Method 2

materials:

1.5N HCl

2.Distilled water

3.pH 6.8 phosphate buffer

4. Giemsa stain: 2 mL stock Giemsa; 4 mL pH 6.8 buffer; 92 mL distilled water



procedure:

1.Place slides in a Coplin jar containing 5N HCl for 10 minutes at room temperature.

2.Rinse with tap water for 10 minutes.

3.Stain for in Giemsa for 10 minutes.

4.Rinse, dry, and coverslip, if desired.

物理状态 :

Solid

溶解度 :

insoluble in water (<0.1%), methanol (5.8 mg/l), and ethanol.

保存 :

Store at room temperature

熔点 :

300° C (lit.)

PubChem CID :

MDL 号码 :

MFCD00081642

EC号码 :

257-438-2

SMILES :

C[N+](=C1C=CC2=NC3=C(C=C(C=C3)N)SC2=C1)C.[Cl-]