Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Gephyrin Antibody (G-6) - Western Blotting - Image 355497
Gephyrin 抗体 (G-6): sc-25311. HeLa (A), 3611-RF (B) 和 NCI-H460 (C) 全细胞裂解液及人脑组织提取物 (D) 中 Gephyrin 表达的 Western 印迹分析.
Gephyrin 抗体 (G-6): sc-25311. HeLa (A), 3611-RF (B) 和 NCI-H460 (C) 全细胞裂解液及人脑组织提取物 (D) 中 Gephyrin 表达的 Western 印迹分析.

Gephyrin 抗体 (G-6): sc-25311

4.6(8)
寫評論提問

说明书
  • Gephyrin 抗体 G-6 是小鼠单克隆 IgG2b κ，Gephyrin抗体, 在18篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Gephyrin的氨基酸437-736
  • Gephyrin 抗体 (G-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Gephyrin
  • 抗Gephyrin抗体（G-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Gephyrin (G-6): sc-25311的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Gephyrin 抗体 (G-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Gephyrin 抗体 (G-6) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Gephyrin抗体（G-6）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Gephyrin蛋白。抗Gephyrin抗体（G-6）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Gephyrin在抑制性甘氨酸受体的突触定位中起着至关重要的作用，而抑制性甘氨酸受体对于维持中枢神经系统的抑制性神经传递至关重要。Gephyrin主要在大脑中表达，在大脑中，Gephyrin定位于神经元突触后膜，促进甘氨酸受体锚定到突触后微管上。Gephyrin的正常功能对于突触的稳定性和可塑性至关重要，因此Gephyrin在调节突触强度以及大脑中兴奋性和抑制性信号的整体平衡方面发挥着关键作用。在大鼠和人类的大脑组织中，至少发现了五种Gephyrin的剪接变体，每种变体都可能对神经信号传导中的不同功能发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Gephyrin 抗体 (G-6) 参考文献:

  1. 受体相关蛋白 gephyrin 的共表达改变了 alpha 2 甘氨酸受体的配体结合亲和力。  |  Takagi, T., et al. 1992. FEBS Lett. 303: 178-80. PMID: 1318846
  2. 一种推定的甘氨酸受体-微管蛋白连接蛋白--ephyrin的初级结构和替代剪接变体。  |  Prior, P., et al. 1992. Neuron. 8: 1161-70. PMID: 1319186
  3. 抑制甘氨酸受体在 GABA 能突触上锚定的与 gephyrin 相关的机制  |  Meier, J. and Grantyn, R. 2004. J Neurosci. 24: 1398-405. PMID: 14960612
  4. gephyrin 动态聚类甘氨酸受体的结构基础  |  Sola, M., et al. 2004. EMBO J. 23: 2510-9. PMID: 15201864
  5. 不同的 gamma2 亚基结构域介导突触后 GABAA 受体和 gephyrin 的聚集和突触功能。  |  Alldred, MJ., et al. 2005. J Neurosci. 25: 594-603. PMID: 15659595
  6. 调节GABA能抑制：Gephyrin分子结构和功能。  |  Pizzarelli, R., et al. 2020. Neuroscience. 439: 125-136. PMID: 31356900
  7. 类真核的gephyrin和同源膜受体协调棒状杆菌的细胞分裂和极性伸长。  |  Martinez, M., et al. 2023. Nat Microbiol. 8: 1896-1910. PMID: 37679597
  8. 鉴定甘氨酸受体 beta 亚基上的 gephyrin 结合基团。  |  Meyer, G., et al. 1995. Neuron. 15: 563-72. PMID: 7546736
  9. gephyrin 转录物在成年和发育中大鼠大脑中的分布。  |  Kirsch, J., et al. 1993. Eur J Neurosci. 5: 1109-17. PMID: 8281317
  10. 小鼠 gephyrin 基因启动子区域分析。  |  Ramming, M., et al. 1997. FEBS Lett. 405: 137-40. PMID: 9089277
  11. 大脑中受体/通道集群分子 gephyrin 的变体：不同的分布模式, 发育特征以及钙蛋白酶的蛋白水解作用。  |  Kawasaki, BT., et al. 1997. J Neurosci Res. 49: 381-8. PMID: 9260749
  12. 突触后甘氨酸受体生物学。  |  Vannier, C. and Triller, A. 1997. Int Rev Cytol. 176: 201-44. PMID: 9394920

订购信息

产品名称产品编号规格价格数量收藏夹

Gephyrin 抗体 (G-6)

sc-25311
200 µg/ml
$316.00

Gephyrin (G-6): m-IgGκ BP-HRP 套装

sc-520773
200 µg Ab, 40 µg BP
$354.00

Gephyrin (G-6): m-IgG2b BP-HRP 套装

sc-548852
200 µg Ab; 10 µg BP
$354.00

Gephyrin 抗体 (G-6) AC

sc-25311 AC
500 µg/ml, 25% agarose
$416.00

Gephyrin 抗体 (G-6) HRP

sc-25311 HRP
200 µg/ml
$316.00

Gephyrin 抗体 (G-6) FITC

sc-25311 FITC
200 µg/ml
$330.00

Gephyrin 抗体 (G-6) PE

sc-25311 PE
200 µg/ml
$343.00

Gephyrin 抗体 (G-6) Alexa Fluor® 488

sc-25311 AF488
200 µg/ml
$357.00

Gephyrin 抗体 (G-6) Alexa Fluor® 546

sc-25311 AF546
200 µg/ml
$357.00

Gephyrin 抗体 (G-6) Alexa Fluor® 594

sc-25311 AF594
200 µg/ml
$357.00

Gephyrin 抗体 (G-6) Alexa Fluor® 647

sc-25311 AF647
200 µg/ml
$357.00

Gephyrin 抗体 (G-6) Alexa Fluor® 680

sc-25311 AF680
200 µg/ml
$357.00

Gephyrin 抗体 (G-6) Alexa Fluor® 790

sc-25311 AF790
200 µg/ml
$357.00

I came across that some of postsynaptic proteins, including gephyrin, required milder fixation for IHC (e.g., 1-2 h 2-4% PFA fixation). If I use this antibody (G-6, sc-25311) for IHC of mouse brain sections, will it be essential to do so?

Asked by: Tatsuya
Thank you for your question. In our testing of the antibody, we have not tried using milder fixation methods and followed our standard protocol to achieve success with this antibody. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2019-01-26

How much protein do you recommend loading on the gel for Western blot detection using Gephyrin (G-6): sc-25311?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 5
Date published: 2017-02-08
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_25311, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 99ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Worked wellWorked well in both IF and WB analyses. IF: Epifluorescent analysis primary hippocampal neurons at day in vitro 15 using Gephyrin (sc-25311; 1:50) (red), GFP (green) and MAP2 (blue). Fixing in 4% PFA + 4% sucrose in PSB for 15 minutes followed 100% methanol post-fixing for 10 minutes at -20*C. WB: Analysis of Gephyrin from P30 mouse brain lysate. Mouse anti-Gephyrin (sc-25311) was diluted 1:1000 in TBS 1x containing 5% skimmed milk and 0,2% Tween-20. Anti-mouse HRP-conjugated secondary antibody was diluted 1:10000.
Date published: 2018-04-13
Rated 4 out of 5 by from gephyrin抗体（G-6）使用感受小鼠海马组织，gephyrin一抗稀释浓度1:1000，4℃孵育过夜，条带清晰，特异性强，背景干净。不错。
Date published: 2018-01-15
Rated 5 out of 5 by from Excellent immunostaining for IHC-P of mouse brainThis antibody worked very well in immunofluorescence of formalin-fixed paraffin embedded mouse brain tissues, antigen retrieval used Pascal Citrate pH6, concentration 1:100.
Date published: 2017-08-15
Rated 5 out of 5 by from Preferred choice!This monoclonal Gephyrin Antibody (G-6) is the preferred choice for Western blotting and immunohistochemistry applications. Great choice!
Date published: 2017-01-26
Rated 4 out of 5 by from Good specificity and signal strength withGood specificity and signal strength with some background bands in BC3H1, SK-N-MC and H4 whole cell lysates. -SCBT QC
Date published: 2015-07-03
Rated 4 out of 5 by from Worked well with transfectedWorked well with transfected, Hela and 293T cells lysate -SCBT QC
Date published: 2015-06-03
Rated 5 out of 5 by from IF: Very good nuclear and cytoplasmic stainingIF: Very good nuclear and cytoplasmic staining in PC-12 cells. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2013-09-28
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_25311, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
Gephyrin 抗体 (G-6) is rated 4.6 out of 5 by 8.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_8
  • loc_zh_CN, sid_25311, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 80ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。