Date published: 2025-9-7
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GAPDH Antibody (G-9) - Western Blotting - Image 387325
用 GAPDH 抗体 (G-9) Alexa Fluor® 680: sc-365062 AF680 检测 Hep G2 (A), HeLa (B), A549 (C), RAW 264. 7 (D) 和 KNRK (E) 全细胞裂解液中的 GAPDH 表达, 用β-Actin (C4) Alexa Fluor® 790: sc-47778 AF790 检测β-Actin表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
用 GAPDH 抗体 (G-9) Alexa Fluor® 680: sc-365062 AF680 检测 Hep G2 (A), HeLa (B), A549 (C), RAW 264. 7 (D) 和 KNRK (E) 全细胞裂解液中的 GAPDH 表达, 用β-Actin (C4) Alexa Fluor® 790: sc-47778 AF790 检测β-Actin表达. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

GAPDH 抗体 (G-9): sc-365062

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说明书
  • GAPDH抗体(G-9)是小鼠单克隆IgG1 κ, 在1,636篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长GAPDH的氨基酸1-335
  • 抗-GAPDH 抗体 (G-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GAPDH and GAPDH-2
  • 抗GAPDH抗体（G-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GAPDH (G-9): sc-365062的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是GAPDH Antibody (G-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与GAPDH Antibody (G-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

GAPDH抗体（G-9）是一种IgG1 κ小鼠单克隆GAPDH抗体，可以通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）、免疫组织化学法（IHC, P）和酶联免疫吸附法（ELISA）检测小鼠、大鼠和人类来源的GAPDH和GAPDH-2。GAPDH抗体（G-9）既有非偶联形式的抗GAPDH抗体，也有多种偶联形式的抗GAPDH抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。GAPDH抗体（G-9）特异性检测甘油醛-3-磷酸脱氢酶（GAPDH），也被称为尿嘧啶DNA糖基化酶。GAPDH蛋白在无机磷酸和烟酰胺腺嘌呤二核苷酸（NAD）存在的情况下，催化甘油醛-3-磷酸的可逆氧化磷酸化，这是碳水化合物代谢中产生能量的重要步骤。尽管GAPDH长期以来被认为在糖酵解中起着不可或缺的作用，但GAPDH的其他功能还包括作为尿嘧啶DNA糖基化酶、激活转录、结合RNA以及参与核RNA输出、DNA复制和DNA修复。GAPDH在肝癌、肺癌和前列腺癌中的表达上调，这表明这些组织是GAPDH抗体（G-9）的合适阳性对照。在细胞凋亡过程中，GAPDH会转移到细胞核中。因此，使用GAPDH抗体（G-9）进行免疫染色可能会导致细胞质或细胞核定位。GAPDH与神经元蛋白复合物有关，这些蛋白涉及人类神经退行性疾病，包括β-淀粉样前体、亨廷顿蛋白和其他三重重复神经元疾病蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GAPDH 抗体 (G-9)

sc-365062
200 µg/ml
$316.00

GAPDH (G-9): m-IgGκ BP-HRP 套装

sc-522129
200 µg Ab, 40 µg BP
$354.00

GAPDH (G-9): m-IgG1 BP-HRP 套装

sc-552050
200 µg Ab; 20 µg BP
$354.00

GAPDH 抗体 (G-9) AC

sc-365062 AC
500 µg/ml, 25% agarose
$416.00

GAPDH 抗体 (G-9) HRP

sc-365062 HRP
200 µg/ml
$316.00

GAPDH 抗体 (G-9) FITC

sc-365062 FITC
200 µg/ml
$330.00

GAPDH 抗体 (G-9) PE

sc-365062 PE
200 µg/ml
$343.00

GAPDH 抗体 (G-9) Alexa Fluor® 488

sc-365062 AF488
200 µg/ml
$357.00

GAPDH 抗体 (G-9) Alexa Fluor® 546

sc-365062 AF546
200 µg/ml
$357.00

GAPDH 抗体 (G-9) Alexa Fluor® 594

sc-365062 AF594
200 µg/ml
$357.00

GAPDH 抗体 (G-9) Alexa Fluor® 647

sc-365062 AF647
200 µg/ml
$357.00

GAPDH 抗体 (G-9) Alexa Fluor® 680

sc-365062 AF680
200 µg/ml
$357.00

GAPDH 抗体 (G-9) Alexa Fluor® 790

sc-365062 AF790
200 µg/ml
$357.00

We are using this antibody on mouse brain samples. We are getting a huge band at around 50kDa along with GAPDH. How can we use this antibody to get only the specific band of GAPDH?

Asked by: Shashank
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service . Please call 800-457-3801 or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-07-17

这款产品二抗用哪个m-igg1还是iggk

Asked by: djxk
感谢您的提问。GAPDH (G-9) 抗体在WB 和 IHC(P) 方面的应用，推荐的二抗检测试剂为m-IgGκ BP-HRP。 Thank you for your question. m-IgGκ BP-HRP is the preferred secondary detection reagent for GAPDH Antibody (G-9) for WB and IHC(P) applications.
Answered by: Technical Service SH
Date published: 2023-10-13

Hello! I am using the antibody GAPDH (G-9) Alexa Fluor 647 (sc-365062) for Immunofluorescence. Could you please share the protocol you used for Immunofluoresnce with cultured cells (not tissue samples). Thank you!

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2023-10-13

why I get two clearly bands around ~55KD and ~40KD in my results，which one is specific to my antibody？thank you

Asked by: kangxin
Thank you for your question. The expected molecular weight for GAPDH is around 37-38kDa, so the band around 40kDa is your expected band.
Answered by: Tech Service
Date published: 2019-12-11

Hi, My western results detecting GAPDH around 30ish. Is it non-specific band? Thanks

Asked by: Uzma
Thank you for your question. The expected molecular weight of GapDH is 37kDa, but it can appear a little higher or lower depending on factors such as gel, markers etc. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2023-10-13

Hi, I have used SCBT Abs a lot. Can you share buffer composition that Abs are in? DTT or reducing agent is quite important for our experiment Thanks

Asked by: DrBio
Thank you for your question. GAPDH (G-9): sc-365062 contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support
Date published: 2023-10-13

How does GAPDH compare to Actin as a protein loading control for time course studies?

Asked by: Professor Griffin
Thank you for your question. Both Actin and GAPDH are generally acceptable as loading controls for time course, RNAi and covalent mod studies. However, their molecular weights are slightly different; Actin (43kda), GAPDH (37kda). In addition, GAPDH is more relevant for non-differentiated lymphocytes, whereas Actin or GAPDH may be suitable for adherents.
Answered by: Technical Support
Date published: 2024-06-18
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Rated 4 out of 5 by from GAPDH Antibody for Cytosolic FractionsThe GAPDH antibody was instrumental in defining cytosolic fractions, and the enriched, well-defined band unequivocally demonstrated its exclusive presence in this fraction. This antibody's specificity significantly contributed to the accuracy of our cytosolic protein analyses.
Date published: 2024-01-04
Rated 5 out of 5 by from Muy buen reconocimiento de la proteínaLo utilicé en una línea celular de mosquito (C6/36) y hubo un buen reconocimiento de la proteína en WB
Date published: 2023-10-09
Rated 5 out of 5 by from GAPDH antibodyanti-glyceraldehyde 3-phosphate dehydrogenase antibody used to probe membrane and aid in monitoring protein expressions.
Date published: 2023-06-30
Rated 5 out of 5 by from a very satisfying antibodyThis antibody works well, the protein bands are very clear and easily to determine. I'm very grateful for the scientific work that has been done with this antibody.
Date published: 2023-02-07
Rated 5 out of 5 by from Worked wellWe used this antibody in brain and peripheral tissues and it worked well.
Date published: 2023-01-06
Rated 5 out of 5 by from Very good antibodyWe have been using this antibody for long time. Its very nice
Date published: 2022-06-27
Rated 5 out of 5 by from Free fatty acid receptor 2This antibody is very good.I want to use it again.
Date published: 2022-05-19
Rated 5 out of 5 by from Nice clear bandsWe use this antibody regularly in my laboratory and it gives nice clear bands, particulalry sharp when doing 12% gels. You can use this at 1:5000.
Date published: 2022-04-07
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GAPDH 抗体 (G-9) is rated 4.7 out of 5 by 70.
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