Date published: 2025-9-7
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GAPDH Antibody (D-6) - Western Blotting - Image 356675
GAPDH 抗体 (D-6): sc-166545. JAR (A), NIH/3T3 (B), Hep G2 (C) 和 KNRK (D) 全细胞裂解液及小鼠肝组织提取物 (E) 中 GAPDH 表达的 Western 印迹分析.
GAPDH 抗体 (D-6): sc-166545. JAR (A), NIH/3T3 (B), Hep G2 (C) 和 KNRK (D) 全细胞裂解液及小鼠肝组织提取物 (E) 中 GAPDH 表达的 Western 印迹分析.

GAPDH 抗体 (D-6): sc-166545

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说明书
  • GAPDH抗体(D-6)是小鼠单克隆IgG2b κ, 在156篇文献中引用，规格为200 µg/ml
  • 与human起源的GAPDH的C-terminus附近的肽图谱
  • 抗-GAPDH 抗体 (D-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GAPDH; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗GAPDH抗体（D-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GAPDH (D-6): sc-166545的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 GAPDH 抗体 (D-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GAPDH 抗体 (D-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

GAPDH抗体（D-6）是一种IgG2b κ小鼠单克隆GAPDH抗体，它可以通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）、免疫组织化学法（IHC, P）和酶联免疫吸附法（ELISA）检测小鼠、大鼠和人类来源的GAPDH。GAPDH抗体（D-6）有非偶联形式的抗GAPDH抗体，也有多种偶联形式的抗GAPDH抗体，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。甘油醛-3-磷酸脱氢酶（GAPDH），也被称为尿嘧啶DNA糖基化酶，在无机磷酸和烟酰胺腺嘌呤二核苷酸（NAD）存在的情况下，催化甘油醛-3-磷酸的可逆氧化磷酸化，这是碳水化合物代谢中产生能量的重要步骤。尽管GAPDH长期以来被认为在糖酵解中起着不可或缺的作用，但GAPDH的其他功能还包括作为尿嘧啶DNA糖基化酶、激活转录、结合RNA以及参与核RNA输出、DNA复制和DNA修复。GAPDH在肝癌、肺癌和前列腺癌中的表达上调。在细胞凋亡过程中，GAPDH会转移到细胞核中。GAPDH与神经元蛋白复合物有关，这些蛋白涉及人类神经退行性疾病，包括β-淀粉样前体、亨廷顿蛋白和其他三重重复神经元疾病蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GAPDH 抗体 (D-6)

sc-166545
200 µg/ml
$316.00

GAPDH (D-6): m-IgGκ BP-HRP 套装

sc-521651
200 µg Ab, 40 µg BP
$354.00

GAPDH (D-6): m-IgG2b BP-HRP 套装

sc-548946
200 µg Ab; 10 µg BP
$354.00

GAPDH 抗体 (D-6) AC

sc-166545 AC
500 µg/ml, 25% agarose
$416.00

GAPDH 抗体 (D-6) HRP

sc-166545 HRP
200 µg/ml
$316.00

GAPDH 抗体 (D-6) FITC

sc-166545 FITC
200 µg/ml
$330.00

GAPDH 抗体 (D-6) PE

sc-166545 PE
200 µg/ml
$343.00

GAPDH 抗体 (D-6) Alexa Fluor® 488

sc-166545 AF488
200 µg/ml
$357.00

GAPDH 抗体 (D-6) Alexa Fluor® 546

sc-166545 AF546
200 µg/ml
$357.00

GAPDH 抗体 (D-6) Alexa Fluor® 594

sc-166545 AF594
200 µg/ml
$357.00

GAPDH 抗体 (D-6) Alexa Fluor® 647

sc-166545 AF647
200 µg/ml
$357.00

GAPDH 抗体 (D-6) Alexa Fluor® 680

sc-166545 AF680
200 µg/ml
$357.00

GAPDH 抗体 (D-6) Alexa Fluor® 790

sc-166545 AF790
200 µg/ml
$357.00

GAPDH (D-6) 中和勝肽

sc-166545 P
100 µg/0.5 ml
$68.00

Hi, Why are two bands showing up for GAPDH in some of the example images but not others? Is there a way to optimize how many bands show up?

Asked by: Is17
Thank you for your question. The antibody expresses multiple isoforms but is commonly found around 37KDa. PMID: 1924305 (37) https://www.uniprot.org/uniprotkb/P16858/entry#sequences
Answered by: BlakeJ
Date published: 2023-11-09

buenas tardes, me preguntaba si hay alguna referencia para justificar el protocolo que la suma de las dos bandas se puede utilizar para cuantificar, si ya decidí y tengo los datos de GAPDH como control de carga.

Asked by: KARINA D
Gracias por su pregunta. Desafortunadamente, no tenemos un protocolo para la cuantificación de proteínas en Western Blot.
Answered by: Tech Support Europe
Date published: 2021-07-23

What is the identity of the two bands? Whether these two bands are isoform?

Asked by: Anonymous
For more information contact Technical Service.
Answered by: Tech Sales
Date published: 2021-07-09

QUE BANDA ES LA QUE SE CUANTIFICA LA DE ABAJO O LA DE ARRIBA?

Asked by: KARINA D
Thank you for your question. GAPDH has two isoforms, both of which may be present in a given sample. Total GAPDH levels are the sum of both bands produced. If you require an antibody against a housekeeping gene for quantification you could opt for an antibody specific against tubulin or actin.
Answered by: Tech Support Europe
Date published: 2020-12-31

I am working with zebrafish samples. Do you know if this antibody will cross-react?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-09
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Rated 5 out of 5 by from Cross reacts with budding yeast lysateCross reacted with a protein at a slightly smaller than expected size from TCA precipitated whole cell lysate from budding yeast S. cerevisiae. 10% SDS-PAGE gel, antibody used at 1:1000
Date published: 2017-10-23
Rated 5 out of 5 by from good commentUsed for western blot analysis of GAPDH expression in A549 whole cell lysates, clean background.
Date published: 2017-04-05
Rated 5 out of 5 by from Superb!!Outstanding WB, IF and IHC staining data; just a great antibody !!
Date published: 2017-01-26
Rated 5 out of 5 by from Versatile AntibodyThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-11
Rated 5 out of 5 by from Great antibodyGreat monoclonal antibody in HeLa cells and A549 cell lysate data. Antibody is very reliable. Hope that such cooperation, the future can continue. Generally speaking, the price is reasonable and the quality is reliable.
Date published: 2017-01-10
Rated 5 out of 5 by from Excellent antibodyExcellent antibody. We used it on HeLa cell lysate by WB (1/1000) with ECL Prime detection
Date published: 2016-09-21
Rated 5 out of 5 by from One of the best we have used for immunofluorescence.
Date published: 2015-05-20
Rated 5 out of 5 by from Great antibody for human prostate paraffinGreat antibody for human prostate paraffin sections, intense nuclear staining. -SCBT QC
Date published: 2015-03-12
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GAPDH 抗体 (D-6) is rated 5.0 out of 5 by 11.
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