Date published: 2025-9-6
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GAPDH Antibody (A-3) - Western Blotting - Image 352729
GAPDH 抗体 (A-3): sc-137179. NIH/3T3 (A), PC-12 (B), A549 (C) 和 Hep G2 (D) 全细胞裂解液中 GAPDH 表达的 Western 印迹分析.
GAPDH 抗体 (A-3): sc-137179. NIH/3T3 (A), PC-12 (B), A549 (C) 和 Hep G2 (D) 全细胞裂解液中 GAPDH 表达的 Western 印迹分析.

GAPDH 抗体 (A-3): sc-137179

4.3(20)
寫評論提問

说明书
  • GAPDH抗体(A-3)是小鼠单克隆IgG1 κ, 在212篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长GAPDH的氨基酸
  • 抗-GAPDH 抗体 (A-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 GAPDH and GAPDH-2
  • GAPDH抗体（A-3）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GAPDH (A-3): sc-137179的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 GAPDH 抗体 (A-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GAPDH 抗体 (A-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

GAPDH抗体（A-3）是一种IgG1 κ小鼠单克隆GAPDH抗体，可以通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）、免疫组织化学法（IHC, P）和酶联免疫吸附法（ELISA）检测小鼠、大鼠和人类来源的GAPDH和GAPDH-2。GAPDH抗体（A-3）既有偶联形式也有非偶联形式的抗GAPDH抗体。甘油醛-3-磷酸脱氢酶（GAPDH），也被称为尿嘧啶DNA糖基化酶，在无机磷酸和烟酰胺腺嘌呤二核苷酸（NAD）存在的情况下，催化甘油醛-3-磷酸的可逆氧化磷酸化，这是碳水化合物代谢中产生能量的重要步骤。尽管GAPDH长期以来被认为在糖酵解中起着不可或缺的作用，但GAPDH的其他功能还包括作为尿嘧啶DNA糖基化酶、激活转录、结合RNA以及参与核RNA输出、DNA复制和DNA修复。GAPDH在肝癌、肺癌和前列腺癌中的表达上调。在细胞凋亡过程中，GAPDH会转移到细胞核中。GAPDH与神经元蛋白复合物有关，这些蛋白涉及人类神经退行性疾病，包括β-淀粉样前体、亨廷顿蛋白和其他三重重复神经元疾病蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GAPDH 抗体 (A-3) 参考文献:

  1. GAPDH的过度表达可诱导转染克隆GAPDH cDNAs的COS-7细胞凋亡。  |  Tajima, H., et al. 1999. Neuroreport. 10: 2029-33. PMID: 10424669
  2. 甘油醛-3-磷酸脱氢酶与细胞凋亡  |  Berry, MD. and Boulton, AA. 2000. J Neurosci Res. 60: 150-4. PMID: 10740219
  3. 母体糖尿病体内和体外高血糖会降低大鼠胚胎的GAPDH活性。  |  Wentzel, P., et al. 2003. Diabetes. 52: 1222-8. PMID: 12716756
  4. 独立于 Exportin 1 的 GAPDH 核输出。  |  Schmitz, HD., et al. 2003. Cell Biol Int. 27: 511-7. PMID: 12842090
  5. 抗GAPDH-2抗体对雌性小鼠生育力的实验免疫学影响。  |  Wang, Y., et al. 2009. Fertil Steril. 92: 2020-7. PMID: 18980760
  6. ）诱导的聚集。  |  Rawat, P., et al. 2012. Biochem Cell Biol. 90: 329-38. PMID: 22292499
  7. CIB1通过AKT和ERK信号传导防止核GAPDH积累和非凋亡性肿瘤细胞死亡。  |  Leisner, TM., et al. 2013. Oncogene. 32: 4017-27. PMID: 22964641
  8. GAPDH对B23/核磷蛋白的S-亚硝基化可保护细胞免受SIAH1-GAPDH死亡级联反应的影响。  |  Lee, SB., et al. 2012. J Cell Biol. 199: 65-76. PMID: 23027902
  9. GOSPEL蛋白增强一氧化氮供体诱导的GAPDH聚集，并抑制NAD（。  |  González, MC., et al. 2016. FEBS Lett. 590: 2210-20. PMID: 27282776
  10. 帕金森病患者的晶状体中GAPDH减少：一种可能的新生物标志物。  |  Klettner, A., et al. 2017. Mov Disord. 32: 459-462. PMID: 27859598

订购信息

产品名称产品编号规格价格数量收藏夹

GAPDH 抗体 (A-3)

sc-137179
200 µg/ml
$316.00

GAPDH (A-3): m-IgG Fc BP-HRP 套装

sc-527127
200 µg Ab; 10 µg BP
$354.00

GAPDH (A-3): m-IgG1 BP-HRP 套装

sc-532500
200 µg Ab; 20 µg BP
$354.00

GAPDH 抗体 (A-3) AC

sc-137179 AC
500 µg/ml, 25% agarose
$416.00

GAPDH 抗体 (A-3) FITC

sc-137179 FITC
200 µg/ml
$330.00

GAPDH 抗体 (A-3) PE

sc-137179 PE
200 µg/ml
$343.00

GAPDH 抗体 (A-3) Alexa Fluor® 488

sc-137179 AF488
200 µg/ml
$357.00

GAPDH 抗体 (A-3) Alexa Fluor® 546

sc-137179 AF546
200 µg/ml
$357.00

GAPDH 抗体 (A-3) Alexa Fluor® 594

sc-137179 AF594
200 µg/ml
$357.00

GAPDH 抗体 (A-3) Alexa Fluor® 647

sc-137179 AF647
200 µg/ml
$357.00

GAPDH 抗体 (A-3) Alexa Fluor® 680

sc-137179 AF680
200 µg/ml
$357.00

GAPDH 抗体 (A-3) Alexa Fluor® 790

sc-137179 AF790
200 µg/ml
$357.00

I need to detect the house keeping GAPDH in WB analysis but don't know which antibody to chose...can you help please

Asked by: mohammad obeid
Thank you for your question. Please get in touch with our technical support team so we can help you find an appropriate antibody for your requirements.
Answered by: Tech Support Europe
Date published: 2020-12-09

Is GAPDH Antibody (A-3): sc-137179 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com Alternatively, consider using GAPDH (0411), which is available conjugated to agarose, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. (https://www.scbt.com/scbt/product/gapdh-antibody-0411)
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from Excellent antibody!Excelent antibody! I have used Anti-GAPDH Antibody (A-3): sc-137179 in western blots using TM4 cells (40 ug protein- 1:200). It gives clear and great results!
Date published: 2022-06-16
Rated 5 out of 5 by from Great features.We used this antibody for western blot in rat brain cytosolic fraction, and we got clear bands.
Date published: 2020-12-29
Rated 1 out of 5 by from No signalNo signal in in muscle cells or tissue. Cell Signaling antibody works fine.
Date published: 2018-02-22
Rated 5 out of 5 by from work nicelyIt works well on vole's brain tissue extraction for western blot.
Date published: 2017-11-28
Rated 4 out of 5 by from Good selectivity but not as sensitiveThis GAPDH has nice selectivity for just GAPDH at 37k Da but it is not as sensitive as other manufacturers. I blocked my blot in 5% BSA and added 1:500 GAPDH overnight at 4 degrees C. The blot had a very faint GAPDH band using Pico detection reagent.
Date published: 2017-11-22
Rated 5 out of 5 by from loading control with anti-GAPDHProtein extracts of mouse mammary gland were analyzed by immunoblot with anti-GAPDH (sc-137179). A single band of the expected molecular weight was detected
Date published: 2017-11-07
Rated 5 out of 5 by from Cross reacts with budding yeast lysateCross reacted with a band at the expected size from TCA precipitated whole cell lysate from budding yeast S. cerevisiae. 10% SDS PAGE gel, antibody used at 1:1000
Date published: 2017-10-23
Rated 1 out of 5 by from GAPDH antibodyI got three anti GAPDH antibody one month ago. Only sc-365062 has bind in 37 kDa for Rat kidney. sc137179 and sc47724 have very strong bind in 70-80kDa, weak bind in 37kDa. I don't know it is normal for Rat kidney of GAPDH MW 70-80 kDa or antibody something happened.
Date published: 2017-10-13
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GAPDH 抗体 (A-3) is rated 4.3 out of 5 by 20.
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