GAPDH Antibody (6F7): sc-51907

GAPDH Antibody (6F7) is a mouse monoclonal IgG1 antibody raised against rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), with species reactivity to human and rabbit. GAPDH is a key enzyme in glycolysis, catalyzing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD⁺), a critical step for energy production in carbohydrate metabolism. Beyond its classical metabolic role, GAPDH exhibits diverse functions including acting as a uracil DNA glycosylase involved in DNA repair, participating in transcriptional activation, binding to RNA, and facilitating nuclear RNA export and DNA replication. GAPDH expression is notably upregulated in various cancers such as liver, lung, and prostate, making GAPDH a significant marker in oncological research. During apoptosis, GAPDH translocates to the nucleus, suggesting involvement in programmed cell death pathways. Additionally, GAPDH interacts with neuronal proteins linked to neurodegenerative disorders, including the β-amyloid precursor protein and huntingtin, highlighting GAPDH′s importance in neurobiology. Anti-GAPDH antibody (6F7) provides a reliable tool for detecting GAPDH in human and rabbit samples, and advances studies in cellular metabolism, disease mechanisms, and therapeutic development.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GAPDH Antibody (6F7) has 61 citations in a variety of scientific publications.

Primary antibodies like Anti-GAPDH Antibody (6F7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GAPDH Antibody (6F7) Specifications

Alternate Product Name: GAPDH

Product Clone Name: 6F7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: GADPH

Epitope Species: rabbit

Product Reactivity/Detection: GAPDH

Species Reactivity/Detection: human, rabbit

Additional Mammalian Reactivity: canine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 61 citations in various scientific publications

Available Conjugates/Forms for GAPDH Antibody (6F7): N/A

The preferred GAPDH antibody is GAPDH Antibody (6C5): sc-32233.