Date published: 2025-9-5

021-6093-6350

SCBT Portrait Logo
Seach Input

GAPDH 抗体 (0411): sc-47724

4.7(255)
寫評論提問

说明书
  • GAPDH 抗体 0411 是小鼠单克隆 IgG1 κ,GAPDH抗体, 在3,428篇文献中引用,规格为200 µg/ml
  • 免疫human物种的重组GAPDH
  • GAPDH 抗体 (0411) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 GAPDH; 不建议用于检测GAPDH of mouse or rat origin
  • 抗GAPDH抗体(0411)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取GAPDH (0411): sc-47724免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 GAPDH 抗体 (0411) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与GAPDH 抗体 (0411) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

GAPDH抗体(0411)是一种小鼠单克隆IgG1 kappa轻链抗体,针对人类来源的重组甘油醛-3-磷酸脱氢酶(GAPDH)产生。GAPDH是糖酵解中的一种关键酶,催化甘油醛-3-磷酸的可逆氧化磷酸化,这是碳水化合物代谢中为细胞产生能量的重要步骤。虽然主要位于细胞质中,但GAPDH可以在细胞凋亡期间转移到细胞核中,参与转录激活、DNA复制和DNA修复等核功能,凸显其在细胞过程中的多功能作用。GAPDH与多种重要蛋白相互作用,包括与阿尔茨海默病发病机制有关的β-淀粉样蛋白前体蛋白(APP)、与亨廷顿病有关的突变蛋白亨廷顿蛋白(Huntingtin)、与神经退行性疾病有关的E3泛素连接酶Siah1,这表明其参与细胞死亡途径。此外,GAPDH表达在肝癌、肺癌和前列腺癌中上调,使这些组织成为GAPDH(0411)单克隆抗体的合适阳性对照。抗GAPDH抗体(0411)可与人类样本发生反应,是免疫印迹(WB)和免疫沉淀(IP)等应用的理想选择。GAPDH单克隆抗体(0411)具有高度特异性和亲和性,是研究人员研究糖酵解途径、GAPDH的核功能、其与疾病相关蛋白的相互作用及其在癌症中的作用时的重要工具,从而显著推进这些关键领域的研究。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

GAPDH 抗体 (0411)

sc-47724
200 µg/ml
$316.00

GAPDH (0411): m-IgG Fc BP-HRP 套装

sc-528511
200 µg Ab; 10 µg BP
$354.00

GAPDH (0411): m-IgGκ BP-HRP 套装

sc-520906
200 µg Ab, 40 µg BP
$354.00

GAPDH (0411): m-IgG1 BP-HRP 套装

sc-542968
200 µg Ab; 20 µg BP
$354.00

GAPDH 抗体 (0411) AC

sc-47724 AC
500 µg/ml, 25% agarose
$416.00

GAPDH 抗体 (0411) HRP

sc-47724 HRP
200 µg/ml
$316.00

GAPDH 抗体 (0411) FITC

sc-47724 FITC
200 µg/ml
$330.00

GAPDH 抗体 (0411) PE

sc-47724 PE
200 µg/ml
$343.00

GAPDH 抗体 (0411) Alexa Fluor® 488

sc-47724 AF488
200 µg/ml
$357.00

GAPDH 抗体 (0411) Alexa Fluor® 546

sc-47724 AF546
200 µg/ml
$357.00

GAPDH 抗体 (0411) Alexa Fluor® 594

sc-47724 AF594
200 µg/ml
$357.00

GAPDH 抗体 (0411) Alexa Fluor® 647

sc-47724 AF647
200 µg/ml
$357.00

GAPDH 抗体 (0411) Alexa Fluor® 680

sc-47724 AF680
200 µg/ml
$357.00

GAPDH 抗体 (0411) Alexa Fluor® 790

sc-47724 AF790
200 µg/ml
$357.00

Hello. I have a question. Which do part of Gapdh (amino acid) make your Anti-GAPDH antibody (0411) sc-47724 product?

Asked by: Khurlee
Apple
Answered by: Charlie
Date published: 2025-06-05

we have GAPDH ANTIBODY SC-47724 (LOT a1821). Is it HRP CONJUGATED?

Asked by: ANURADHA
Thank you for your question. Lot A1821 is for the unconjugated form.
Answered by: BlakeJ
Date published: 2024-09-26

What is the amino acid sequence that this GAPDH(0411) recognizes?

Asked by: Vishal Prajapat
Thank you for your question. No epitope mapping has been performed for this antibody as of yet. Its exact binding site is not known.
Answered by: Tech Support Europe
Date published: 2024-01-09

I have not can detect GAPDH-488 (sc-47724 AF488) Can you help me with the protocol?

Asked by: JessicaM
Thank you for your question. Please reach out to our technical service team directly for assistance with your experiment.
Answered by: Tech Support Europe
Date published: 2022-11-30

Hello, This is my first time doing WB, I was wondering if I could get a sample of GAPDH Antibody (0411): sc-47724 to test? I would love to purchase after testing it on our sample? Thanks

Asked by: Anonymous
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a FREE 10 µg sample of this product.
Answered by: Technical Service 3
Date published: 2024-12-12

Has anyone had successful results using this antibody for the detection of mouse GAPDH?

Asked by: DF60
Thank you for your question. This antibody is not recommended for detection of GAPDH of mouse or rat origin. We have several options of alternative GAPDH antibodies for mouse and rat. We recommend, sc-365062: GAPDH (G-9), which has published Western blot data in mouse and rat on our website. Plus, 40 citations each for mouse and rat.
Answered by: Technical Support
Date published: 2024-09-26

I have a GAPDH antibody SC-20357 (lot# G1807). how old is it? what is the expiration time of your antibodies?

Asked by: Josee
Thank you for your question. This lot was released within the last few months. We do not characterize an expiration date for our antibodies. They can last for several years when stored properly at 4 degrees C (and never frozen). We do warranty our antibodies for one year from the date of purchase.
Answered by: Technical Support
Date published: 2021-08-22

about GAPDH antibody SC-47724, what volume per tube? I know it is 200 ug/ml but I can't find the amount send. thanks

Asked by: Josee
Thank you for your question. We provide this antibody as 200 µg in 1 mL volume.
Answered by: Technical Support
Date published: 2025-01-08
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_9
  • loc_zh_CN, sid_47724, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 103ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Great GAPDH AntibodyGreat GAPDH Antibody (GAPDH (0411), mouse monoclonal Ab. sc-47724; LOT# J0824). The shipping to Thailand was really fast. The quality is good at a reasonable price.
Date published: 2025-05-06
Rated 5 out of 5 by from Reliable loading controlWe regularly use GAPDH as a loading control, and it is always reliable.
Date published: 2024-08-07
Rated 5 out of 5 by from GAPDH works well for WBGAPDH works well for WB in d20 (hESC beta-like) clusters at 1/5000.
Date published: 2024-05-27
Rated 5 out of 5 by from Excelent antibodyThe antibody works wells in western blot. When HT29 cell lysates were used a clear band was detected with the anti-Gapdh at the dilution 1:500.
Date published: 2024-02-20
Rated 1 out of 5 by from Used to work but no longer doesUsed this happily for several years but latest batch is not properly conjugated to HRP. No band unless I use a secondary antibody. Lab manager tried to resolve with company but they are not being forthcoming.
Date published: 2024-02-13
Rated 1 out of 5 by from No hubo reconocimiento de la proteinaLo utilicé en una línea celular de mosquito (C6/36) y no hubo reconocimiento de la proteína
Date published: 2023-10-09
Rated 5 out of 5 by from GAPDH ANTIBOYThe antibody is good on western blot. The band is clean and clear on western blot.
Date published: 2023-08-14
Rated 5 out of 5 by from Works very wellWe bought this one 1 month ago. This antibody works very well in Western blot.
Date published: 2023-06-21
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_256
  • loc_zh_CN, sid_47724, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT
GAPDH 抗体 (0411) is rated 4.7 out of 5 by 256.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_256
  • loc_zh_CN, sid_47724, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 104ms
  • REVIEWS, PRODUCT