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GAPDH-2 Antibody (2E3-2E10) - Western Blotting - Image 384295
GAPDH-2 抗体 (2E3-2E10): sc-293335. MCF7 (A), NTERA-2 cl. D1 (B) 和 NIH/3T3 (C) 全细胞裂解液及人类睾丸组织提取物 (D) 中 GAPDH-2 表达的 Western 印迹分析.
GAPDH-2 抗体 (2E3-2E10): sc-293335. MCF7 (A), NTERA-2 cl. D1 (B) 和 NIH/3T3 (C) 全细胞裂解液及人类睾丸组织提取物 (D) 中 GAPDH-2 表达的 Western 印迹分析.

GAPDH-2 抗体 (2E3-2E10): sc-293335

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寫評論提問

说明书
  • GAPDH-2抗体(2E3-2E10)是小鼠单克隆IgG1 κ, 在101篇文献中引用，规格为100 µg/ml
  • 免疫human物种的GAPDH-2的氨基酸1-408
  • 推荐用于 mouse, rat 和 human 来源的GAPDH-2 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取GAPDH-2 (2E3-2E10): sc-293335的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成GAPDH-2 抗体 (2E3-2E10)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

GAPDH-2抗体（2E3-2E10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类样本中的GAPDH-2，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。GAPDH-2 (2E3-2E10) 抗体以非结合形式提供，可进行多种实验设计。甘油醛-3-磷酸脱氢酶（GAPDH）通过催化甘油醛-3-磷酸的可逆氧化磷酸化在细胞代谢中发挥关键作用，这是糖酵解的关键步骤，以烟酰胺腺嘌呤二核苷酸（NAD）的形式产生能量。除了能量代谢，GAPDH还参与各种细胞过程，包括DNA修复和转录调控，这凸显了GAPDH在维持细胞稳态中的重要性。在包括肝癌、肺癌和前列腺癌在内的多种癌症中，GAPDH的表达通常会上调。在细胞凋亡过程中，GAPDH会转移到细胞核中，与与神经退行性疾病相关的蛋白质（如β-淀粉样蛋白前体和亨廷顿蛋白）相互作用，进一步强调了GAPDH在正常生理和疾病中的多方面作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GAPDH-2 抗体 (2E3-2E10) 参考文献:

  1. 工程糖酵解甘油醛-3-磷酸脱氢酶与 NAD+ 烟酸酰胺的反构象结合，但不会发生 A 型特异性氢化物转移。  |  Eyschen, J., et al. 1999. Arch Biochem Biophys. 364: 219-27. PMID: 10190977
  2. 对一种古老蛋白质的新认识：哺乳动物甘油醛-3-磷酸脱氢酶的功能多样性。  |  Sirover, MA. 1999. Biochim Biophys Acta. 1432: 159-84. PMID: 10407139
  3. 甘油醛-3-磷酸脱氢酶与细胞凋亡  |  Berry, MD. and Boulton, AA. 2000. J Neurosci Res. 60: 150-4. PMID: 10740219
  4. 甘油醛-3-磷酸脱氢酶在神经变性和细胞凋亡信号转导中的作用。  |  Tatton, WG., et al. 2000. J Neural Transm Suppl. 77-100. PMID: 11205159
  5. 正位胰腺肿瘤模型细胞系的转录谱分析揭示了转移相关基因。  |  Tarbé, N., et al. 2001. Anticancer Res. 21: 3221-8. PMID: 11848476
  6. 甘油醛-3-磷酸脱氢酶细胞内结构和功能的改变：神经退行性疾病的共同表型？  |  Mazzola, JL. and Sirover, MA. 2002. Neurotoxicology. 23: 603-9. PMID: 12428732
  7. 病理晚期人类前列腺癌中甘油醛-3-磷酸脱氢酶基因表达增加。  |  Rondinelli, RH., et al. 1997. Prostate Cancer Prostatic Dis. 1: 66-72. PMID: 12496918
  8. 人类核尿嘧啶 DNA 糖基化酶是甘油醛-3-磷酸脱氢酶的 37-kDa 亚基。  |  Meyer-Siegler, K., et al. 1991. Proc Natl Acad Sci U S A. 88: 8460-4. PMID: 1924305
  9. 低压缺氧和复氧诱导大鼠大脑皮层蛋白质组谱变化  |  Hernández, R., et al. 2013. Neuromolecular Med. 15: 82-94. PMID: 22961459
  10. 脊柱融合过程中脂肪因子的时间表达。  |  Virk, S., et al. 2017. Spine J. 17: 1897-1906. PMID: 28647583
  11. [穿心莲内酯对脂多糖刺激下大鼠 II 型肺泡上皮细胞促凝血因子和纤溶抑制因子表达的影响]。  |  Yang, G., et al. 2021. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 33: 155-160. PMID: 33729132
  12. [Notch3信号通路调控主动脉夹层血管干细胞向平滑肌细胞分化的机制]。  |  Han, Y., et al. 2023. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 35: 503-508. PMID: 37308231

订购信息

产品名称产品编号规格价格数量收藏夹

GAPDH-2 抗体 (2E3-2E10)

sc-293335
100 µg/ml
$316.00

which kind of secondary anti-mouse IgG reagents was recommended for GAPDH-2 Antibody (2E3-2E10): sc-293335 by means of weatern blot？ 问题是GAPDH-2 Antibody (2E3-2E10): sc-293335的二抗用哪种，网页推荐那个失效了

Asked by: nafapple
感谢您的提问。做WB的话可以试一下sc-516102， https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp 关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. Here is m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-04-23

Can sc-293335: GAPDH-2 (2E3-2E10) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, sc-293335: GAPDH-2 (2E3-2E10) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Works goodI bought this antibody for western blots and works good for me
Date published: 2019-01-25
Rated 5 out of 5 by from Good antibodyIt is a good antibody. You can choose it to complete your experiment.
Date published: 2017-09-26
Rated 5 out of 5 by from greatI have used this antobody in western blot, the bands is specific at 36kDa and it can be easily tested in cell and tissue lysis .The recommed dilution is1:3000- 1:5000.
Date published: 2017-01-02
Rated 5 out of 5 by from GOOD FOR WESTERN BLOTThis antobody is good for western blot, it can be used at a dilution of 1:3000, I test the expression of GAPDH in different liver cancer cell line, tha band is specific, the result is very good.
Date published: 2016-12-17
Rated 5 out of 5 by from GOOD antibodySingle strong band in WB assays.WB1:5000 is OK!GOOD antibody
Date published: 2016-12-06
Rated 5 out of 5 by from a good loading cantrol antibodyactin,GAPDH and tubulin are usual loading control antibody.these antibody maybe the most uesd antibody.this GAPDH antibody has a good perfomance in WB.i also use it in IF and IHC,also work well.
Date published: 2016-11-20
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GAPDH-2 抗体 (2E3-2E10) is rated 5.0 out of 5 by 6.
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