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GAPDH-2 Antibody (2E3-2E10) - Western Blotting - Image 384295
GAPDH-2 抗体 (2E3-2E10): sc-293335. MCF7 (A), NTERA-2 cl. D1 (B) 和 NIH/3T3 (C) 全细胞裂解液及人类睾丸组织提取物 (D) 中 GAPDH-2 表达的 Western 印迹分析.
GAPDH-2 抗体 (2E3-2E10): sc-293335. MCF7 (A), NTERA-2 cl. D1 (B) 和 NIH/3T3 (C) 全细胞裂解液及人类睾丸组织提取物 (D) 中 GAPDH-2 表达的 Western 印迹分析.

GAPDH-2 抗体 (2E3-2E10): sc-293335

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寫評論提問
说明书
  • GAPDH-2抗体(2E3-2E10)是小鼠单克隆IgG1 κ, 在101篇文献中引用，规格为100 µg/ml
  • 免疫human物种的GAPDH-2的氨基酸1-408
  • 推荐用于 mouse, rat 和 human 来源的GAPDH-2 WB, IP, IF, IHC(P) 和 ELISA检测
  • 目前，我们还没有完成GAPDH-2 抗体 (2E3-2E10)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

GAPDH-2抗体（2E3-2E10）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类样本中的GAPDH-2，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。GAPDH-2 (2E3-2E10) 抗体以非结合形式提供，可进行多种实验设计。甘油醛-3-磷酸脱氢酶（GAPDH）通过催化甘油醛-3-磷酸的可逆氧化磷酸化在细胞代谢中发挥关键作用，这是糖酵解的关键步骤，以烟酰胺腺嘌呤二核苷酸（NAD）的形式产生能量。除了能量代谢，GAPDH还参与各种细胞过程，包括DNA修复和转录调控，这凸显了GAPDH在维持细胞稳态中的重要性。在包括肝癌、肺癌和前列腺癌在内的多种癌症中，GAPDH的表达通常会上调。在细胞凋亡过程中，GAPDH会转移到细胞核中，与与神经退行性疾病相关的蛋白质（如β-淀粉样蛋白前体和亨廷顿蛋白）相互作用，进一步强调了GAPDH在正常生理和疾病中的多方面作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

GAPDH-2 抗体 (2E3-2E10) 参考文献:

  1. 工程糖酵解甘油醛-3-磷酸脱氢酶与 NAD+ 烟酸酰胺的反构象结合，但不会发生 A 型特异性氢化物转移。  |  Eyschen, J., et al. 1999. Arch Biochem Biophys. 364: 219-27. PMID: 10190977
  2. 对一种古老蛋白质的新认识：哺乳动物甘油醛-3-磷酸脱氢酶的功能多样性。  |  Sirover, MA. 1999. Biochim Biophys Acta. 1432: 159-84. PMID: 10407139
  3. 甘油醛-3-磷酸脱氢酶与细胞凋亡  |  Berry, MD. and Boulton, AA. 2000. J Neurosci Res. 60: 150-4. PMID: 10740219
  4. 甘油醛-3-磷酸脱氢酶在神经变性和细胞凋亡信号转导中的作用。  |  Tatton, WG., et al. 2000. J Neural Transm Suppl. 77-100. PMID: 11205159
  5. 正位胰腺肿瘤模型细胞系的转录谱分析揭示了转移相关基因。  |  Tarbé, N., et al. 2001. Anticancer Res. 21: 3221-8. PMID: 11848476
  6. 甘油醛-3-磷酸脱氢酶细胞内结构和功能的改变：神经退行性疾病的共同表型？  |  Mazzola, JL. and Sirover, MA. 2002. Neurotoxicology. 23: 603-9. PMID: 12428732
  7. 病理晚期人类前列腺癌中甘油醛-3-磷酸脱氢酶基因表达增加。  |  Rondinelli, RH., et al. 1997. Prostate Cancer Prostatic Dis. 1: 66-72. PMID: 12496918
  8. 人类核尿嘧啶 DNA 糖基化酶是甘油醛-3-磷酸脱氢酶的 37-kDa 亚基。  |  Meyer-Siegler, K., et al. 1991. Proc Natl Acad Sci U S A. 88: 8460-4. PMID: 1924305
  9. 低压缺氧和复氧诱导大鼠大脑皮层蛋白质组谱变化  |  Hernández, R., et al. 2013. Neuromolecular Med. 15: 82-94. PMID: 22961459
  10. 脊柱融合过程中脂肪因子的时间表达。  |  Virk, S., et al. 2017. Spine J. 17: 1897-1906. PMID: 28647583
  11. [穿心莲内酯对脂多糖刺激下大鼠 II 型肺泡上皮细胞促凝血因子和纤溶抑制因子表达的影响]。  |  Yang, G., et al. 2021. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 33: 155-160. PMID: 33729132
  12. [Notch3信号通路调控主动脉夹层血管干细胞向平滑肌细胞分化的机制]。  |  Han, Y., et al. 2023. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 35: 503-508. PMID: 37308231

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产品名称产品编号规格价格数量收藏夹

GAPDH-2 抗体 (2E3-2E10)

sc-293335
100 µg/ml
$322.00

which kind of secondary anti-mouse IgG reagents was recommended for GAPDH-2 Antibody (2E3-2E10): sc-293335 by means of weatern blot？ 问题是GAPDH-2 Antibody (2E3-2E10): sc-293335的二抗用哪种，网页推荐那个失效了

Asked by: nafapple
感谢您的提问。做WB的话可以试一下sc-516102， https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp 关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. Here is m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-04-23

Can sc-293335: GAPDH-2 (2E3-2E10) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, sc-293335: GAPDH-2 (2E3-2E10) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by DSSS from Works goodI bought this antibody for western blots and works good for me
Date published: 2019-01-25
Rated 5 out of 5 by Shu Zhuo from Good antibodyIt is a good antibody. You can choose it to complete your experiment.
Date published: 2017-09-26
Rated 5 out of 5 by zhangxiaodou from greatI have used this antobody in western blot, the bands is specific at 36kDa and it can be easily tested in cell and tissue lysis .The recommed dilution is1:3000- 1:5000.
Date published: 2017-01-02
Rated 5 out of 5 by qxrsyhg from GOOD FOR WESTERN BLOTThis antobody is good for western blot, it can be used at a dilution of 1:3000, I test the expression of GAPDH in different liver cancer cell line, tha band is specific, the result is very good.
Date published: 2016-12-17
Rated 5 out of 5 by houjing3132 from GOOD antibodySingle strong band in WB assays.WB1:5000 is OK!GOOD antibody
Date published: 2016-12-06
Rated 5 out of 5 by Kate Zhao from a good loading cantrol antibodyactin,GAPDH and tubulin are usual loading control antibody.these antibody maybe the most uesd antibody.this GAPDH antibody has a good perfomance in WB.i also use it in IF and IHC,also work well.
Date published: 2016-11-20
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GAPDH-2 抗体 (2E3-2E10) is rated 5.0 out of 5 by 6.
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