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G<sub>α s</sub> Antibody (12) - Western Blotting - Image 353715 — G_αs (12): sc-135914. 3T3-L1 (A), C3H/10T1/2 (B) 和 C6 (C) 全细胞裂解液以及大鼠大脑 (D), 大鼠小脑 (E) 和小鼠大脑 (F) 组织提取物中 G_αs 表达的 Western 印迹分析.

G_αs (12): sc-135914. 3T3-L1 (A), C3H/10T1/2 (B) 和 C6 (C) 全细胞裂解液以及大鼠大脑 (D), 大鼠小脑 (E) 和小鼠大脑 (F) 组织提取物中 G_αs 表达的 Western 印迹分析.

G_{α s} 抗体 (12): sc-135914

Name: G&alpha; s抗体 (12)
Brand: SCBT
SKU: sc-135914
Price: 316.0 USD
Availability: InStock

4.7(11)

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说明书

G_{α s}抗体(12)是小鼠单克隆IgG₁ κ, 在13篇文献中引用，规格为200 µg/ml
免疫human物种的G_{α s}的氨基酸11-21
推荐用于 mouse, rat 和 human 来源的G_{α s} WB 和 IP检测
还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
关于如何获取G_{α s} (12): sc-135914的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG₁ BP-HRP是G_{α s} Antibody (12) for WB applications. 的首选辅助检测试剂，这些试剂现与G_{α s} Antibody (12) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(13)

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G_{α s} 抗体 (12) 参考文献:

不同的 beta 亚基决定了 G 蛋白与跨膜受体的相互作用。 | Kleuss, C., et al. 1992. Nature. 358: 424-6. PMID: 1322501
G 蛋白 gamma 亚基的选择性组织分布，包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。 | Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
在 Sf9 细胞中合成的 G 蛋白 beta γ 亚基。G 蛋白 beta γ 亚基在 Sf9 细胞中合成。 | Iñiguez-Lluhi, JA., et al. 1992. J Biol Chem. 267: 23409-17. PMID: 1429682
异三聚体 GTP 结合蛋白 beta 亚基的多样性：新型 beta 亚基 cDNA 的特征。 | von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
Gustducin 是一种味觉细胞特异性 G 蛋白，与转导蛋白关系密切。 | McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
信号转导中 G 蛋白的多样性。 | Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
G 蛋白α S 亚基通过蛋白激酶 A 信号转导促进肾细胞癌细胞增殖。 | Zhang, B., et al. 2017. DNA Cell Biol. 36: 237-242. PMID: 28051330
母体 GNAS 以细胞类型特异性的方式促进特大 G 蛋白 α 亚基（XLαs）的表达。 | Cui, Q., et al. 2021. Front Genet. 12: 680537. PMID: 34220953
超越G蛋白α亚基：研究Gαi3异三聚体其他成分的功能影响。 | Rysiewicz, B., et al. 2023. Cell Commun Signal. 21: 279. PMID: 37817242
信号转导的选择性由异源三聚体 G 蛋白的 gamma 亚基决定。 | Kleuss, C., et al. 1993. Science. 259: 832-4. PMID: 8094261
与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。 | Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779
Golf：参与气味信号转导的嗅觉神经元特异性-G 蛋白。 | Jones, DT. and Reed, RR. 1989. Science. 244: 790-5. PMID: 2499043

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
G_{α s} 抗体 (12)	sc-135914	200 µg/ml	$316.00
G_{α s} (12): m-IgG Fc BP-HRP 套装	sc-527103	200 µg Ab; 10 µg BP	$354.00
G_{α s} (12): m-IgG₁ BP-HRP 套装	sc-532476	200 µg Ab; 20 µg BP	$354.00
Gα s 抗体 (12) AC	sc-135914 AC	500 µg/ml, 25% agarose	$416.00
Gα s 抗体 (12) HRP	sc-135914 HRP	200 µg/ml	$316.00

Does this antibody react with zebrafish Gαs? If not experimentally validated, what is the predicted reactivity against the zebrafish protein?

Asked by: Lulu97

Thank you for your question. Based on our tests with this product, Anti-Gα s Antibody (12): sc-135914 is only recommended for detection of Gα s of mouse, rat and human origin by WB and IP. We do not have any internal data to support reactivity in samples from zebrafish.

Answered by: Tech Support Europe

Date published: 2022-09-19

Hi, Is this MAb specific for Gα-s? because the description of MAb for Gα-olf says, it can recognize both forms s and olf and in our study, we need to identify which one of the two is involved, so specificity is very necessary.

Asked by: ASM25

Thank you for your question. The antibody was raised against amino acids 11-21 of G s of human origin. The exact binding region of this antibody within the antigen has not been mapped. This antibody has not been tested experimentally for potential cross-reactivity under denaturing conditions in a Western Blot or native conditions in IP against different proteins. You might want to align the antigen sequence against the protein form of interest in order to determine potential reactivity. based on the antigen sequence we do not expect relevant cross-reactivity against G -olf. Please contact our Technical Service by phone or email or by Live Chat if you have any further questions.

Answered by: SCBT Heidelberg Support

Date published: 2018-11-27

Hi, How can I have a 10ul trial sample of the Gα s Antibody (12): sc-135914 antibody. Thanks

Asked by: Zhong Ding

Thank you for your inquiry. For a limited time, we are offering a free 10 µg sample of this antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.

Answered by: Technical Support 15

Date published: 2018-02-01

For Western Blot, is it recommended to use denatured or non-denatured conditions with this antibody?

Asked by: TinTin

Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.

Answered by: Technical Support 9

Date published: 2017-01-12

Can you recommend a positive control to use with Gα s (12): sc-135914 for Western blot?

Asked by: TinTin

We recommend Jurkat Whole Cell Lysate (sc-2204) or rat or mouse brain tissue extract (sc-2392 or sc-2253) as positive controls as we have Western blot data showing specific detection of Gα s in these samples.

Answered by: Technical Support 9

Date published: 2017-01-12

Rated 4 out of 5 by KDP2 from Strong Antibody Signal for WBWe tried a sample antibody on WB, signal was strong, but we got two bands.

Date published: 2017-11-03

Rated 5 out of 5 by Shine_ from no problemThe WB of the antibody was detected in the tissues of rat cerebellum, the signals were clear, specific and unique.

Date published: 2017-10-26

Rated 5 out of 5 by 33wen from Worked goodThis antibody sc-135914 works very well in WB, TBS 5% milk, diluted 1/250.

Date published: 2017-08-25

Rated 5 out of 5 by liujia from works wellIt works well in western blotting at dilution of 1:500 analysis Human liver tissue .

Date published: 2017-02-25

Rated 3 out of 5 by Professor Griffin from Moderate specificityGalpha-S (12/sc-135914): Moderate specificity: detects multiple bands from HEK293 lysate, of which one is the correct MW for Galpha-S. Poor sensitivity - may be optimized.

Date published: 2017-02-09

Rated 5 out of 5 by Bill28 from Superb westernVery nice western blot results; strong signal with low backgrond

Date published: 2017-01-31

Rated 5 out of 5 by Cweed from Great for WBA proven reagent for WB detection. It provides great signal and clear specific bands. It is reactive with mouse, rat, and human and has been published with several times.

Date published: 2017-01-30

Rated 5 out of 5 by MC from Various expression levels of the G s proteinVarious expression levels of the G s protein is demonstrated by Western Blot using Jurkat, HEK293, TT, K562 and MCF-7 whole cell lysates. -SCBT QC

Date published: 2015-04-12

G α s 抗体 (12) Reviews - page 2

G α s 抗体 (12) is rated 4.7 out of 5 by 11.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	GNAS	Entrez Gene ID: 2778	20q13.32	XM_017027812, XM_017027813, XM_017027814, XM_017027815, XM_017027816, XM_017027817, XM_017027818, XM_017027819, XM_017027820, XM_017027821, XM_017027822, NM_000516, NM_001077488, NM_001077489, NM_001077490, NM_001309840, NM_001309842, NM_001309861, NM_001309883, NM_016592, NM_080425, NM_080426	UniProt ID: O95467	603233
Mouse	Gnas	Entrez Gene ID: 14683	2 H4	XM_006498774, XM_006498776, XM_006498777, XM_006498778, XM_006498779, XM_006498780, XM_017315658, NM_001077507, NM_001077510, NM_001310083, NM_001310085, NM_010309, NM_019690, NM_022000, NM_201616, NM_201617, NM_201618, NM_010310	UniProt ID: P63094	N/A

G_{α s} 抗体 (12): sc-135914

G_{α s} 抗体 (12) 参考文献:

G_{α s} 抗体 (12) 规格

替代产品名称: G protein subunit GNAS

产品克隆名称: 12

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 11-21

抗原表位靶标: G_{α s}

抗原表位种属: human

产品反应性/检测: G_{α s}

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 13篇各种科学出版物的引文

G_{α s} 抗体 (12)可选的偶联物/形式: AC: Agarose, HRP: Horseradish Peroxidase

G_{α s} 抗体 (12)

G_{α s} (12): m-IgG Fc BP-HRP 套装

G_{α s} (12): m-IgG₁ BP-HRP 套装

Gα s 抗体 (12) AC

Gα s 抗体 (12) HRP

Does this antibody react with zebrafish Gαs? If not experimentally validated, what is the predicted reactivity against the zebrafish protein?

Hi, Is this MAb specific for Gα-s? because the description of MAb for Gα-olf says, it can recognize both forms s and olf and in our study, we need to identify which one of the two is involved, so specificity is very necessary.

Hi, How can I have a 10ul trial sample of the Gα s Antibody (12): sc-135914 antibody. Thanks

For Western Blot, is it recommended to use denatured or non-denatured conditions with this antibody?

Can you recommend a positive control to use with Gα s (12): sc-135914 for Western blot?

Gα s 抗体 (12): sc-135914

Gα s 抗体 (12) 参考文献:

Gα s 抗体 (12) 规格

替代产品名称: G protein subunit GNAS

产品克隆名称: 12

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 11-21

抗原表位靶标: Gα s

抗原表位种属: human

产品反应性/检测: Gα s

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 13篇各种科学出版物的引文

Gα s 抗体 (12)可选的偶联物/形式: AC: Agarose, HRP: Horseradish Peroxidase

Gα s 抗体 (12)

Gα s (12): m-IgG Fc BP-HRP 套装

Gα s (12): m-IgG1 BP-HRP 套装

Gα s 抗体 (12) AC

Gα s 抗体 (12) HRP

Does this antibody react with zebrafish G&alpha;s? If not experimentally validated, what is the predicted reactivity against the zebrafish protein?

Hi, Is this MAb specific for G&alpha;-s? because the description of MAb for G&alpha;-olf says, it can recognize both forms s and olf and in our study, we need to identify which one of the two is involved, so specificity is very necessary.

Hi, How can I have a 10ul trial sample of the G&alpha; s Antibody (12): sc-135914 antibody. Thanks

For Western Blot, is it recommended to use denatured or non-denatured conditions with this antibody?

Can you recommend a positive control to use with G&alpha; s (12): sc-135914 for Western blot?

G_{α s} 抗体 (12): sc-135914

G_{α s} 抗体 (12) 参考文献:

G_{α s} 抗体 (12) 规格

抗体同型: IgG₁ kappa light chain

抗原表位靶标: G_{α s}

产品反应性/检测: G_{α s}

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

G_{α s} 抗体 (12)可选的偶联物/形式: AC: Agarose, HRP: Horseradish Peroxidase

G_{α s} 抗体 (12)

G_{α s} (12): m-IgG Fc BP-HRP 套装

G_{α s} (12): m-IgG₁ BP-HRP 套装

Does this antibody react with zebrafish Gαs? If not experimentally validated, what is the predicted reactivity against the zebrafish protein?

Hi, Is this MAb specific for Gα-s? because the description of MAb for Gα-olf says, it can recognize both forms s and olf and in our study, we need to identify which one of the two is involved, so specificity is very necessary.

Hi, How can I have a 10ul trial sample of the Gα s Antibody (12): sc-135914 antibody. Thanks

Can you recommend a positive control to use with Gα s (12): sc-135914 for Western blot?