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G<sub>α o</sub> Antibody (A2) - Western Blotting - Image 11360 — G_{α o} (A2): sc-13532. SK-N-SH (A) 和 IMR-32 (B) 全细胞裂解液以及大鼠脑 (C) 和小鼠脑 (D) 组织提取物中 G_{α o} 表达的 Western 印迹分析.

Gα o Antibody (A2) - Western Blotting - Image 11360 — G_{α o} (A2): sc-13532. SK-N-SH (A) 和 IMR-32 (B) 全细胞裂解液以及大鼠脑 (C) 和小鼠脑 (D) 组织提取物中 G_{α o} 表达的 Western 印迹分析.

Gα o Antibody (A2) - Western Blotting - Image 4322 — G_{α o} (A2): sc-13532. 小鼠 (A) 和大鼠 (B) 脑提取物中 G_αo 表达的 Western 印迹分析.

Gα o Antibody (A2) - Western Blotting - Image 394042 — Gα o 抗体 (A2) HRP: sc-13532 HRP. 大鼠脑组织提取物中 Gα o 表达的直接 Western 印迹分析.

G_{α o} (A2): sc-13532. SK-N-SH (A) 和 IMR-32 (B) 全细胞裂解液以及大鼠脑 (C) 和小鼠脑 (D) 组织提取物中 G_{α o} 表达的 Western 印迹分析.

G_{α o} 抗体 (A2): sc-13532

Name: G&alpha; o抗体 (A2)
Brand: SCBT
SKU: sc-13532
Price: 316.0 USD
Availability: InStock

4.9(8)

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说明书

G_{α o}抗体(A2)是小鼠单克隆IgG₁ κ, 在42篇文献中引用，规格为200 µg/ml
针对G_αo（bovine起源）
抗-G alpha o 抗体 (A2) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 G_{α o}; 也和以下物种反应，包括： bovine
抗G alpha o抗体（A2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取G_{α o} (A2): sc-13532的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG₁ BP-HRP是G_{α o} Antibody (A2) for WB applications. 的首选辅助检测试剂，这些试剂现与G_{α o} Antibody (A2) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(42)

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G_{α o} 抗体 (A2) 参考文献:

G 蛋白 gamma 亚基的选择性组织分布，包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。 | Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
急性运动通过泛素-蛋白酶体蛋白水解途径改变大鼠脂肪细胞中 Galphai2 蛋白的表达。 | Ogasawara, J., et al. 2004. Biochem Biophys Res Commun. 323: 1109-15. PMID: 15381114
异三聚体 GTP 结合蛋白 beta 亚基的多样性：新型 beta 亚基 cDNA 的特征。 | von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
Gustducin 是一种味觉细胞特异性 G 蛋白，与转导蛋白关系密切。 | McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
信号转导中 G 蛋白的多样性。 | Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
三种刺激性 GTP 结合蛋白的生化特征。大, 小 Gs 和嗅觉特异性 G 蛋白 Golf。 | Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
G蛋白G（α）o对小鼠的咽鼓管功能和攻击行为至关重要。 | Chamero, P., et al. 2011. Proc Natl Acad Sci U S A. 108: 12898-903. PMID: 21768373
在运动障碍的病理生理学中，Gαo 是 cAMP 信号的主要决定因素。 | Muntean, BS., et al. 2021. Cell Rep. 34: 108718. PMID: 33535037
小鼠小脑中 G 蛋白 Gαo 亚基的细胞多样性和差异亚细胞定位 | Roldán-Sastre, A., et al. 2021. Front Neuroanat. 15: 686279. PMID: 34248508
神经元抑制性沉默因子限制肌细胞 GαO 表达并对心衰进展具有保护作用 | Sossalla, S. and Bers, DM. 2022. Circ Res. 130: 249-251. PMID: 35050687
G 蛋白α o 亚基：保守半胱氨酸的突变确定了亚基接触面并改变了 GDP 亲和力。 | Thomas, TC., et al. 1993. Proc Natl Acad Sci U S A. 90: 10295-9. PMID: 8234290
与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。 | Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
G_{α o} 抗体 (A2)	sc-13532	200 µg/ml	$316.00
G_{α o} (A2): m-IgG Fc BP-HRP 套装	sc-526554	200 µg Ab; 10 µg BP	$354.00
G_{α o} (A2): m-IgG₁ BP-HRP 套装	sc-531927	200 µg Ab; 20 µg BP	$354.00
Gα o 抗体 (A2) AC	sc-13532 AC	500 µg/ml, 25% agarose	$416.00
Gα o 抗体 (A2) HRP	sc-13532 HRP	200 µg/ml	$316.00
Gα o 抗体 (A2) FITC	sc-13532 FITC	200 µg/ml	$330.00
Gα o 抗体 (A2) PE	sc-13532 PE	200 µg/ml	$343.00
Gα o 抗体 (A2) Alexa Fluor^® 488	sc-13532 AF488	200 µg/ml	$357.00
G_{α o} 抗体 (A2) Alexa Fluor^® 546	sc-13532 AF546	200 µg/ml	$357.00
Gα o 抗体 (A2) Alexa Fluor^® 594	sc-13532 AF594	200 µg/ml	$357.00
Gα o 抗体 (A2) Alexa Fluor^® 647	sc-13532 AF647	200 µg/ml	$357.00
Gα o 抗体 (A2) Alexa Fluor^® 680	sc-13532 AF680	200 µg/ml	$357.00
Gα o 抗体 (A2) Alexa Fluor^® 790	sc-13532 AF790	200 µg/ml	$357.00

Hello, I would like to know, against which Gao epitope was this antibody (Gα o Antibody (A2): sc-13532 ) raised? Is that rather N terminal or C terminal part of Gao? I could not find the information on your webpage. Thank you, Jana

Asked by: Jana Val

Thank you for your question. Gα o Antibody (A2): sc-13532 was raised against full-length Gαo of bovine origin. Its exact epitope region has not been determined yet, so I am afraid we currently do not have any information on the antibody's epitope.

Answered by: Tech Support Europe

Date published: 2020-06-15

Hi, I am wondering if this antibody can capture Drosophila Galphao protein from Drosophila S2 cells by western blot? Please let me know if you have ever seen publications about that. Many thanks.

Asked by: greenygreen

Thank you for your question. We are not aware of any publications at this time using this antibody in drosophila samples.

Answered by: Technical Support

Date published: 2019-03-07

Can Gα o (A2): sc-13532 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt

Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Gα o (A2): sc-13532 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.

Answered by: Technical Support

Date published: 2017-02-24

Rated 5 out of 5 by SantoshBalot from Worked well in IPI used this antibody to pull down G alpha o from mouse brain lysate and it worked well.

Date published: 2018-05-08

Rated 4 out of 5 by Eduardo Molina from Good antibodyI have used anti-Gαo (A2) sc-13532 in Western blot with mouse brain extracts and cultured neurons at 1:500 and it works great.

Date published: 2018-02-19

Rated 5 out of 5 by silvama3 from EXCELENT!works great for western blot and IHC in rat retina.

Date published: 2017-02-14

Rated 5 out of 5 by Germaine from Produced a lot of publishable WB dataWith over 20 citations in the primary literature for use in WB, IF and IHC in mouse, rat and human samples, this proves to be a reliable and well characterized Galpha o monoclonal antibody.

Date published: 2017-01-24

Rated 5 out of 5 by LM from Worked well in WB in SkWorked well in WB in Sk-N-SH, IMR-32 whole cell lysates and rat and mouse brain tissue extracts. -SCBT QC

Date published: 2015-05-17

Rated 5 out of 5 by JT from Good WB antibody with strong band in transfectedGood WB antibody with strong band in transfected HEK-293 and P1-14 cell lines. -SCBT Publication Review

Date published: 2015-04-19

Rated 5 out of 5 by MS from Worked well in IF in mouse eye sampleWorked well in IF in mouse eye sample. -SCBT Publication Review

Date published: 2015-02-18

Rated 5 out of 5 by SF from Good specificity in WB in chicken embryoGood specificity in WB in chicken embryo samples at 1:200 dilution. -SCBT Publication Review

Date published: 2014-05-22

G α o 抗体 (A2) is rated 4.9 out of 5 by 8.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	GNAO1	Entrez Gene ID: 2775	16q12.2	XM_011523003, NM_020988, NM_138736	UniProt ID: P09471	139311
Mouse	Gnao1	Entrez Gene ID: 14681	8 C5	XM_011248311, NM_001113384, NM_010308	UniProt ID: P18872	N/A

G_{α o} 抗体 (A2): sc-13532

G_{α o} 抗体 (A2) 参考文献:

G_{α o} 抗体 (A2) 规格

替代产品名称: G protein subunit GNAO

产品克隆名称: A2

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸:

抗原表位靶标: Gα o

抗原表位种属: bovine

产品反应性/检测: G_{α o}

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: bovine

其它非哺乳动物反应性: N/A

应用: WB, IP, IF

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 42篇各种科学出版物的引文

G_{α o} 抗体 (A2)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 G_{α o} 抗體是 G_{α o} 抗體 (E-1): sc-393874.

G_{α o} 抗体 (A2)

G_{α o} (A2): m-IgG Fc BP-HRP 套装

G_{α o} (A2): m-IgG₁ BP-HRP 套装

Gα o 抗体 (A2) AC

Gα o 抗体 (A2) HRP

Gα o 抗体 (A2) FITC

Gα o 抗体 (A2) PE

Gα o 抗体 (A2) Alexa Fluor^® 488

G_{α o} 抗体 (A2) Alexa Fluor^® 546

Gα o 抗体 (A2) Alexa Fluor^® 594

Gα o 抗体 (A2) Alexa Fluor^® 647

Gα o 抗体 (A2) Alexa Fluor^® 680

Gα o 抗体 (A2) Alexa Fluor^® 790

Hello, I would like to know, against which Gao epitope was this antibody (Gα o Antibody (A2): sc-13532 ) raised? Is that rather N terminal or C terminal part of Gao? I could not find the information on your webpage. Thank you, Jana

Hi, I am wondering if this antibody can capture Drosophila Galphao protein from Drosophila S2 cells by western blot? Please let me know if you have ever seen publications about that. Many thanks.

Can Gα o (A2): sc-13532 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Gα o 抗体 (A2): sc-13532

Gα o 抗体 (A2) 参考文献:

Gα o 抗体 (A2) 规格

替代产品名称: G protein subunit GNAO

产品克隆名称: A2

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸:

抗原表位靶标: Gα o

抗原表位种属: bovine

产品反应性/检测: Gα o

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: bovine

其它非哺乳动物反应性: N/A

应用: WB, IP, IF

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 42篇各种科学出版物的引文

Gα o 抗体 (A2)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor® 488, AF546: Alexa Fluor® 546, AF594: Alexa Fluor® 594, AF647: Alexa Fluor® 647, AF680: Alexa Fluor® 680, AF790: Alexa Fluor® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 Gα o 抗體是 Gα o 抗體 (E-1): sc-393874.

Gα o 抗体 (A2)

Gα o (A2): m-IgG Fc BP-HRP 套装

Gα o (A2): m-IgG1 BP-HRP 套装

Gα o 抗体 (A2) AC

Gα o 抗体 (A2) HRP

Gα o 抗体 (A2) FITC

Gα o 抗体 (A2) PE

Gα o 抗体 (A2) Alexa Fluor® 488

Gα o 抗体 (A2) Alexa Fluor® 546

Gα o 抗体 (A2) Alexa Fluor® 594

Gα o 抗体 (A2) Alexa Fluor® 647

Gα o 抗体 (A2) Alexa Fluor® 680

Gα o 抗体 (A2) Alexa Fluor® 790

Hello, I would like to know, against which Gao epitope was this antibody (G&alpha; o Antibody (A2): sc-13532 ) raised? Is that rather N terminal or C terminal part of Gao? I could not find the information on your webpage. Thank you, Jana

Hi, I am wondering if this antibody can capture Drosophila Galphao protein from Drosophila S2 cells by western blot? Please let me know if you have ever seen publications about that. Many thanks.

Can G&alpha; o (A2): sc-13532 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

G_{α o} 抗体 (A2): sc-13532

G_{α o} 抗体 (A2) 参考文献:

G_{α o} 抗体 (A2) 规格

抗体同型: IgG₁ kappa light chain

产品反应性/检测: G_{α o}

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

G_{α o} 抗体 (A2)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 G_{α o} 抗體是 G_{α o} 抗體 (E-1): sc-393874.

G_{α o} 抗体 (A2)

G_{α o} (A2): m-IgG Fc BP-HRP 套装

G_{α o} (A2): m-IgG₁ BP-HRP 套装

Gα o 抗体 (A2) Alexa Fluor^® 488

G_{α o} 抗体 (A2) Alexa Fluor^® 546

Gα o 抗体 (A2) Alexa Fluor^® 594

Gα o 抗体 (A2) Alexa Fluor^® 647

Gα o 抗体 (A2) Alexa Fluor^® 680

Gα o 抗体 (A2) Alexa Fluor^® 790

Hello, I would like to know, against which Gao epitope was this antibody (Gα o Antibody (A2): sc-13532 ) raised? Is that rather N terminal or C terminal part of Gao? I could not find the information on your webpage. Thank you, Jana

Can Gα o (A2): sc-13532 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?