Date published: 2025-9-6
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G<sub>&alpha; i-1</sub>/GNAI1 Antibody (R4) - Western Blotting - Image 374318
Gα i-1 抗体 (R4): sc-13533. 大鼠大脑 (A), 小鼠大脑 (B) 和人类大脑皮层 (C) 组织提取物中 Gα i-1 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Gα i-1 抗体 (R4): sc-13533. 大鼠大脑 (A), 小鼠大脑 (B) 和人类大脑皮层 (C) 组织提取物中 Gα i-1 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Gα i-1/GNAI1 抗体 (R4): sc-13533

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说明书
  • Gα i-1/GNAI1抗体(R4)是小鼠单克隆IgG2b κ, 在35篇文献中引用，规格为200 µg/ml
  • 免疫rat物种的Gα i-1
  • 抗-Gα i-1/GNAI1 抗体 (R4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和bovine 来源的 Gα i-1
  • 抗Gα i-1/GNAI1抗体（R4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Gα i-1/GNAI1 (R4): sc-13533的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Gα i-1/GNAI1 抗体 (R4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Gα i-1/GNAI1 抗体 (R4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Heterotrimeric G Protein 抗体 包括 G α 11, G α 12, G α 13, G α i1, G α i2, G α i3, G α o, G α olf, G α q和G α q11

Gα i-1抗体（R4）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠、人类和牛的Gα i-1。抗Gα i-1抗体（R4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Gα i-1作为异三聚体G蛋白家族的一员，在细胞信号传导中发挥着至关重要的作用，该家族负责将信号从细胞表面受体传递到细胞内效应器。具体而言，Gα i-1参与抑制腺苷酸环化酶活性，从而降低环状AMP水平，这对于调节神经递质释放、激素分泌和细胞生长等各种生理过程至关重要。Gα i-1与特定受体和下游效应物的相互作用可以调节多种信号通路，因此Gα i-1成为心血管健康、神经生物学和癌症等领域研究的重要目标。了解Gα i-1的功能和相互作用有助于深入了解疾病机理和潜在的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Gα i-1/GNAI1 抗体 (R4) 参考文献:

  1. 免疫沉淀大鼠脑中高亲和力、嘌呤核苷酸敏感、可溶化的μ-阿片受体：G蛋白G（αo）、G（αi1）和G（αi3）的共沉淀。  |  Chalecka-Franaszek, E., et al. 2000. J Neurochem. 74: 1068-78. PMID: 10693938
  2. G蛋白α(i1)氨基末端螺旋与Gbetagamma亚基解离并激活后的构象变化。  |  Medkova, M., et al. 2002. Biochemistry. 41: 9962-72. PMID: 12146960
  3. 伽马（i）（1）的十四烷基氨基末端在溶液中伽马（i）（1）亚基的结构和功能中起着关键作用。  |  Preininger, AM., et al. 2003. Biochemistry. 42: 7931-41. PMID: 12834345
  4. G 蛋白 gamma 亚基的选择性组织分布，包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。  |  Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
  5. 异三聚体 GTP 结合蛋白 beta 亚基的多样性：新型 beta 亚基 cDNA 的特征。  |  von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
  6. Gustducin 是一种味觉细胞特异性 G 蛋白，与转导蛋白关系密切。  |  McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
  7. 信号转导中 G 蛋白的多样性。  |  Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
  8. G{alpha}i1蛋白的GDP-GTP交换过程取决于添加的洗涤剂的类型和浓度，会加速或减慢。  |  Kubota, M., et al. 2009. J Biochem. 146: 875-80. PMID: 19703944
  9. 核结合蛋白1是G{alpha}i1的一种钙调节鸟苷核苷酸解离抑制剂。  |  Kapoor, N., et al. 2010. J Biol Chem. 285: 31647-60. PMID: 20679342
  10. 三种刺激性 GTP 结合蛋白的生化特征。大, 小 Gs 和嗅觉特异性 G 蛋白 Golf。  |  Jones, DT., et al. 1990. J Biol Chem. 265: 2671-6. PMID: 2105931
  11. 人类鸟嘌呤核苷酸结合蛋白α i1亚基的基因在囊性纤维化基因座附近。  |  Bloch, DB., et al. 1988. Am J Hum Genet. 42: 884-8. PMID: 3130752
  12. 与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。  |  Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

订购信息

产品名称产品编号规格价格数量收藏夹

Gα i-1/GNAI1 抗体 (R4)

sc-13533
200 µg/ml
$316.00

Gα i-1/GNAI1 (R4): m-IgG Fc BP-HRP 套装

sc-528286
200 µg Ab; 10 µg BP
$354.00

Gα i-1/GNAI1 (R4): m-IgGκ BP-HRP 套装

sc-520625
200 µg Ab, 40 µg BP
$354.00

Gα i-1/GNAI1 (R4): m-IgG2b BP-HRP 套装

sc-550024
200 µg Ab; 10 µg BP
$354.00

Gα i-1/GNAI1 抗体 (R4) AC

sc-13533 AC
500 µg/ml, 25% agarose
$416.00

Gα i-1/GNAI1 抗体 (R4) HRP

sc-13533 HRP
200 µg/ml
$316.00

Gα i-1/GNAI1 抗体 (R4) FITC

sc-13533 FITC
200 µg/ml
$330.00

Gα i-1/GNAI1 抗体 (R4) PE

sc-13533 PE
200 µg/ml
$343.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 488

sc-13533 AF488
200 µg/ml
$357.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 546

sc-13533 AF546
200 µg/ml
$357.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 594

sc-13533 AF594
200 µg/ml
$357.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 647

sc-13533 AF647
200 µg/ml
$357.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 680

sc-13533 AF680
200 µg/ml
$357.00

Gα i-1/GNAI1 抗体 (R4) Alexa Fluor® 790

sc-13533 AF790
200 µg/ml
$357.00

Has this antibody tested if it cross-react with the drosophila ortholog Gai. Thanks

Asked by: curious researcher
Thank you for your question.G&alpha; i-1 (R4) is recommended for mouse,rat. human and bovine. There is a 77%homology in drosophila. We only warranty if the homology is 95% or higher.
Answered by: BlakeJ
Date published: 2023-05-09

Is sc-13533 (G&alpha; i-1 -R4-) tested for cross-reactivity with G&alpha;0 and G&alpha;-i 2 &amp; 3 isoforms

Asked by: Miguelon
Thank you for your question. sc-13533 is specific for G&alpha; i-1, and should not cross-react with any other G&alpha; forms.
Answered by: Tech Service
Date published: 2020-02-11

In which region of G&alpha; i-1 does the antibody for G&alpha; i-1 (R4): sc-13533 recognize (epitope region)?

Asked by: Alex2323
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2018-12-21

Can G&alpha; i-1 (R4): sc-13533 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: jenniferc
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, G&alpha; i-1 (R4): sc-13533 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from antibody works for us, detected purified Gα i-1This antibody works for us in western blot. Used dilution is 1:200, protein was detected at the corresponding position on the blot, the lowest amount we loaded in our experiment is 10ng
Date published: 2025-06-17
Rated 4 out of 5 by from Works for IFI used this for IF staining brain sections and it works well
Date published: 2017-08-15
Rated 5 out of 5 by from Good operationThis antibody is suitable for many cell lines, I used it to detect Gα i-1, which was derived from WB mouse, and achieved good results.
Date published: 2017-05-23
Rated 5 out of 5 by from The catalystThe test catalyst, adenylate cyclase, each of a very broad range of receptors specifically detects extracellular stimuli, whereas the effectors used to generate one or more intracellular messengers are less
Date published: 2017-02-09
Rated 5 out of 5 by from Very popular choice for WB!This monoclonal G alpha i-1 Antibody (R4) is an excellent choice for Western blotting, IF and IHC(P). Published over a dozen times, especially popular in the literature for Western blotting applications.
Date published: 2017-02-07
Rated 5 out of 5 by from Recommended useublished data for IP and WB in mouse melanocytes,great western blot results,strong signal with low background,also nice IHC data.
Date published: 2017-02-07
Rated 5 out of 5 by from Great antibody for WBThis antibody is really great. In the experiment, the analysis results of Western blot were very strong and clear, and the baseband is perfect.antibody diluted at 1:400.
Date published: 2017-01-26
Rated 5 out of 5 by from reliableIt worked for me from the first attempt with a 1:1000 dilution, this has worked very well in WB, it is predictable and reliable.
Date published: 2017-01-12
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Gα i-1/GNAI1 抗体 (R4) is rated 4.6 out of 5 by 13.
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