登陆账户

购物车 (0)

G<sub>α 12</sub> Antibody (E-12) - Western Blotting - Image 360093 — Gα 12 (E-12): sc-515445. 在 RAW 264. 7 (A), 3T3-L1 (B), H19-7/IGF-IR (C) 和 NIH/3T3 (D) 全细胞裂解液以及小鼠心脏 (E) 和大鼠脑 (F) 组织提取物中 Gα 12 表达的 Western 印迹分析.

Gα 12 (E-12): sc-515445. 在 RAW 264. 7 (A), 3T3-L1 (B), H19-7/IGF-IR (C) 和 NIH/3T3 (D) 全细胞裂解液以及小鼠心脏 (E) 和大鼠脑 (F) 组织提取物中 Gα 12 表达的 Western 印迹分析.

G_{α 12} 抗体 (E-12): sc-515445

Name: G&alpha; 12抗体 (E-12)
Brand: SCBT
SKU: sc-515445
Price: 316.0 USD
Availability: InStock

3.5(4)

寫評論提問

说明书

G alpha 12 抗体 E-12 是小鼠单克隆 IgG₁ κ，G_{α 12}抗体, 在17篇文献中引用，规格为200 µg/ml
免疫原氨基酸序列89-300位于human物种的G_{α 12}的内部区域内
G alpha 12 抗体 (E-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 G_{α 12}
抗G alpha 12抗体（E-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取G_{α 12} (E-12): sc-515445的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG₁ BP-HRP和m-IgGκ BP-HRP是G_{α 12} Antibody (E-12) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与G_{α 12} Antibody (E-12) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(17)

快捷链接

G_{α 12} 抗体 (E-12) 参考文献:

G 蛋白 gamma 亚基的选择性组织分布，包括通过 cDNA 克隆发现的一种新形式的 gamma 亚基。 | Cali, JJ., et al. 1992. J Biol Chem. 267: 24023-7. PMID: 1385432
异三聚体 GTP 结合蛋白 beta 亚基的多样性：新型 beta 亚基 cDNA 的特征。 | von Weizsäcker, E., et al. 1992. Biochem Biophys Res Commun. 183: 350-6. PMID: 1543505
Gustducin 是一种味觉细胞特异性 G 蛋白，与转导蛋白关系密切。 | McLaughlin, SK., et al. 1992. Nature. 357: 563-9. PMID: 1608467
信号转导中 G 蛋白的多样性。 | Simon, MI., et al. 1991. Science. 252: 802-8. PMID: 1902986
Gα 12 和 Gα 13 亚基定义了第四类 G 蛋白α亚基。 | Strathmann, MP. and Simon, MI. 1991. Proc Natl Acad Sci U S A. 88: 5582-6. PMID: 1905812
G 蛋白家族的多样性：小鼠另外五个α亚基的序列。 | Strathmann, M., et al. 1989. Proc Natl Acad Sci U S A. 86: 7407-9. PMID: 2508088
由miR-16失调引起的Gα12过度表达通过促进肝星状细胞中的自噬作用，导致肝纤维化。 | Kim, KM., et al. 2018. J Hepatol. 68: 493-504. PMID: 29080810
肾损伤和疾病中的异源三聚体Gα12/13蛋白。 | Tutunea-Fatan, E., et al. 2020. Am J Physiol Renal Physiol. 318: F660-F672. PMID: 31984793
代谢性疾病中的 Gα12/13 信号传导 | Yang, YM., et al. 2020. Exp Mol Med. 52: 896-910. PMID: 32576930
ER应激引起的肝细胞中Gα12的过度表达会通过ROCK1介导的miR-15a和ALOX12失调加剧急性肝损伤。 | Tak, J., et al. 2022. Theranostics. 12: 1570-1588. PMID: 35198058
Gα12信号调节转录和表型反应，促进胶质母细胞瘤的肿瘤侵袭。 | Chaim, OM., et al. 2023. Sci Rep. 13: 22412. PMID: 38104152
与 G beta gamma, 受体和效应器相互作用的 G alpha 亚基的结构元素。 | Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
G_{α 12} 抗体 (E-12)	sc-515445	200 µg/ml	$316.00
G_{α 12} (E-12): m-IgG Fc BP-HRP 套装	sc-531370	200 µg Ab; 10 µg BP	$354.00
G_{α 12} (E-12): m-IgGκ BP-HRP 套装	sc-525044	200 µg Ab, 40 µg BP	$354.00
G_{α 12} (E-12): m-IgG₁ BP-HRP 套装	sc-544509	200 µg Ab; 20 µg BP	$354.00
Gα 12 抗体 (E-12) AC	sc-515445 AC	500 µg/ml, 25% agarose	$416.00
Gα 12 抗体 (E-12) HRP	sc-515445 HRP	200 µg/ml	$316.00
Gα 12 抗体 (E-12) FITC	sc-515445 FITC	200 µg/ml	$330.00
Gα 12 抗体 (E-12) PE	sc-515445 PE	200 µg/ml	$343.00
Gα 12 抗体 (E-12) Alexa Fluor^® 488	sc-515445 AF488	200 µg/ml	$357.00
G_{α 12} 抗体 (E-12) Alexa Fluor^® 546	sc-515445 AF546	200 µg/ml	$357.00
Gα 12 抗体 (E-12) Alexa Fluor^® 594	sc-515445 AF594	200 µg/ml	$357.00
Gα 12 抗体 (E-12) Alexa Fluor^® 647	sc-515445 AF647	200 µg/ml	$357.00
Gα 12 抗体 (E-12) Alexa Fluor^® 680	sc-515445 AF680	200 µg/ml	$357.00
Gα 12 抗体 (E-12) Alexa Fluor^® 790	sc-515445 AF790	200 µg/ml	$357.00

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-515445: Gå 12 (E-12) mouse monoclonal antibody will work?

Asked by: Randy McDonald

Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.

Answered by: Technical Support

Date published: 2017-03-06

Hello I was wondering whether the sc-515445 G12 antibody has been tested for immunohistochemistry (IHC)? I see that this technique is not in the recommended applications list. Thanks Vernita

Asked by: Vernita

Thank you for your question. This antibody has not been tested for IHC on formalin fixed paraffin embedded tissue sections, so it is not warrantied for that use. It may work, but we have not tested it in house yest so we can not warranty it. However, we do warranty it for IF on cells and IHC on fresh frozen tissue section fixed in methanol or acetone. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com

Answered by: Tech Service

Date published: 2016-11-18

Rated 5 out of 5 by SCBT QC Lab from Good for Western BlotAntibody works for Western blot with 293T cell lysate; expression knocked out with Gα 12 siRNA in published research. -SCBT QC

Date published: 2023-09-14

Rated 1 out of 5 by CapLab22 from High WB background & poor customer serviceWe've tested this in western blot, using HepG2, RAW 264.7 (which should express Ga12), and THP-1 cell lines at 1:500, and in KG-1 and HL-60 at 1:200. In both cases we had very high background and are not confident even in the RAW line that we have a positive band. We contacted Santa Cruz twice to discuss troubleshooting options and have not received any replies.

Date published: 2022-03-10

Rated 3 out of 5 by Gilly from High background bands.We tested this antibody in endothelial cells. The isoforms were detectable, however, there was a very high level of background bands.

Date published: 2019-06-20

Rated 5 out of 5 by liyang86 from upennGα 12 Antibody is very good. The band is very stronger.

Date published: 2018-04-19

G α 12 抗体 (E-12) is rated 3.5 out of 5 by 4.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	GNA12	Entrez Gene ID: 2768	7p22.3	XM_011515288, XM_017012008, NM_001282440, NM_001282441, NM_001293092, NM_007353	UniProt ID: Q03113	604394
Mouse	Gna12	Entrez Gene ID: 14673	5 G2	NM_010302	UniProt ID: P27600	N/A

G_{α 12} 抗体 (E-12): sc-515445

G_{α 12} 抗体 (E-12) 参考文献:

G_{α 12} 抗体 (E-12) 规格

替代产品名称: G-protein subunit GNA12

产品克隆名称: E-12

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 89-300

抗原表位靶标: G_{α 12}

抗原表位种属: human

产品反应性/检测: G_{α 12}

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 17篇各种科学出版物的引文

G_{α 12} 抗体 (E-12)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

G_{α 12} 抗体 (E-12)

G_{α 12} (E-12): m-IgG Fc BP-HRP 套装

G_{α 12} (E-12): m-IgGκ BP-HRP 套装

G_{α 12} (E-12): m-IgG₁ BP-HRP 套装

Gα 12 抗体 (E-12) AC

Gα 12 抗体 (E-12) HRP

Gα 12 抗体 (E-12) FITC

Gα 12 抗体 (E-12) PE

Gα 12 抗体 (E-12) Alexa Fluor^® 488

G_{α 12} 抗体 (E-12) Alexa Fluor^® 546

Gα 12 抗体 (E-12) Alexa Fluor^® 594

Gα 12 抗体 (E-12) Alexa Fluor^® 647

Gα 12 抗体 (E-12) Alexa Fluor^® 680

Gα 12 抗体 (E-12) Alexa Fluor^® 790

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-515445: Gå 12 (E-12) mouse monoclonal antibody will work?

Hello I was wondering whether the sc-515445 G12 antibody has been tested for immunohistochemistry (IHC)? I see that this technique is not in the recommended applications list. Thanks Vernita

Gα 12 抗体 (E-12): sc-515445

Gα 12 抗体 (E-12) 参考文献:

Gα 12 抗体 (E-12) 规格

替代产品名称: G-protein subunit GNA12

产品克隆名称: E-12

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 89-300

抗原表位靶标: Gα 12

抗原表位种属: human

产品反应性/检测: Gα 12

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 17篇各种科学出版物的引文

Gα 12 抗体 (E-12)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor® 488, AF546: Alexa Fluor® 546, AF594: Alexa Fluor® 594, AF647: Alexa Fluor® 647, AF680: Alexa Fluor® 680, AF790: Alexa Fluor® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

Gα 12 抗体 (E-12)

Gα 12 (E-12): m-IgG Fc BP-HRP 套装

Gα 12 (E-12): m-IgGκ BP-HRP 套装

Gα 12 (E-12): m-IgG1 BP-HRP 套装

Gα 12 抗体 (E-12) AC

Gα 12 抗体 (E-12) HRP

Gα 12 抗体 (E-12) FITC

Gα 12 抗体 (E-12) PE

Gα 12 抗体 (E-12) Alexa Fluor® 488

Gα 12 抗体 (E-12) Alexa Fluor® 546

Gα 12 抗体 (E-12) Alexa Fluor® 594

Gα 12 抗体 (E-12) Alexa Fluor® 647

Gα 12 抗体 (E-12) Alexa Fluor® 680

Gα 12 抗体 (E-12) Alexa Fluor® 790

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-515445: G&aring; 12 (E-12) mouse monoclonal antibody will work?

Hello I was wondering whether the sc-515445 G12 antibody has been tested for immunohistochemistry (IHC)? I see that this technique is not in the recommended applications list. Thanks Vernita

G_{α 12} 抗体 (E-12): sc-515445

G_{α 12} 抗体 (E-12) 参考文献:

G_{α 12} 抗体 (E-12) 规格

抗体同型: IgG₁ kappa light chain

抗原表位靶标: G_{α 12}

产品反应性/检测: G_{α 12}

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

G_{α 12} 抗体 (E-12)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

G_{α 12} 抗体 (E-12)

G_{α 12} (E-12): m-IgG Fc BP-HRP 套装

G_{α 12} (E-12): m-IgGκ BP-HRP 套装

G_{α 12} (E-12): m-IgG₁ BP-HRP 套装

Gα 12 抗体 (E-12) Alexa Fluor^® 488

G_{α 12} 抗体 (E-12) Alexa Fluor^® 546

Gα 12 抗体 (E-12) Alexa Fluor^® 594

Gα 12 抗体 (E-12) Alexa Fluor^® 647

Gα 12 抗体 (E-12) Alexa Fluor^® 680

Gα 12 抗体 (E-12) Alexa Fluor^® 790

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-515445: Gå 12 (E-12) mouse monoclonal antibody will work?