Date published: 2025-9-8

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galectin-3 抗体 (M3/38): sc-23938

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说明书
  • galectin-3 抗体 M3/38 是大鼠单克隆 IgG2a, 在59篇文献中引用,提供 200 µg/ml
  • 免疫mouse物种的Galectin-3
  • 抗-galectin-3 抗体 (M3/38) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 Galectin-3
  • 抗galectin-3抗体(M3/38)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取galectin-3 (M3/38): sc-23938免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成galectin-3 抗体 (M3/38)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Galectin-3抗体(M3/38)是一种鼠单克隆IgG2a抗体,专门针对galectin-3,其别名包括Mac-2、hMac-2(人类Mac-2)、GALBP(半乳糖结合蛋白)、CBP35(碳水化合物结合蛋白35)和LGALS3(凝集素,半乳糖苷结合,可溶性,3)。抗半乳糖凝集素-3抗体(M3/38)是针对小鼠来源的半乳糖凝集素-3而制备的,建议用于检测小鼠、大鼠和人类样本中的半乳糖凝集素-3,可通过多种应用,包括蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)。Galectin-3是可溶性β-半乳糖苷结合凝集素家族中的关键成员,在调节细胞间粘附以及细胞与细胞外基质(ECM)之间的相互作用方面发挥着重要作用,而细胞间粘附和细胞与细胞外基质之间的相互作用对于组织发育、免疫反应和细胞通讯等过程至关重要。Galectin-3既存在于细胞内(细胞质和细胞核中),也存在于细胞外(细胞外基质中),其细胞定位对于其多种功能至关重要。在细胞内,galectin-3参与信号传导通路、前mRNA剪接和细胞凋亡,而在细胞外,galectin-3促进细胞粘附、迁移以及与ECM的相互作用。这些功能使galectin-3成为维持细胞稳态和应对细胞应激的不可或缺的组成部分。重要的是,galectin-3通过影响癌细胞增殖、抗凋亡和血管生成而与肿瘤进展和转移有关,从而使其成为癌症研究的重要靶标。Galectin-3 (M3/38) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 以及各种 Alexa Fluor® 结合物,为不同的实验应用提供了多种选择。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

galectin-3 抗体 (M3/38)

sc-23938
200 µg/ml
$316.00

galectin-3 抗体 (M3/38) AC

sc-23938 AC
500 µg/ml, 25% agarose
$416.00

galectin-3 抗体 (M3/38) HRP

sc-23938 HRP
200 µg/ml
$316.00

galectin-3 抗体 (M3/38) FITC

sc-23938 FITC
200 µg/ml
$330.00

galectin-3 抗体 (M3/38) PE

sc-23938 PE
200 µg/ml
$343.00

galectin-3 抗体 (M3/38) Alexa Fluor® 488

sc-23938 AF488
200 µg/ml
$357.00

galectin-3 抗体 (M3/38) Alexa Fluor® 546

sc-23938 AF546
200 µg/ml
$357.00

galectin-3 抗体 (M3/38) Alexa Fluor® 594

sc-23938 AF594
200 µg/ml
$357.00

galectin-3 抗体 (M3/38) Alexa Fluor® 647

sc-23938 AF647
200 µg/ml
$357.00

galectin-3 抗体 (M3/38) Alexa Fluor® 680

sc-23938 AF680
200 µg/ml
$357.00

galectin-3 抗体 (M3/38) Alexa Fluor® 790

sc-23938 AF790
200 µg/ml
$357.00

Hello, can I confirm that this antibody recognizes an epitope on the the N-terminal of Gal-3? Thanks!

Asked by: Jun Ong
Thank you for your question. sc-23938 recognizes an epitope in the N-terminal.
Answered by: BlakeJ
Date published: 2024-10-03

Hello, can I confirm that this antibody recognizes an epitope on the the N-terminal (aa 40 - 100) of Gal-3? Thanks!

Asked by: Jun Ong
Thank you for your question. sc-23938 recognizes an epitope in the N-terminal. Here is a link for more information on the protein : https://www.uniprot.org/uniprotkb/P16110/entry
Answered by: BlakeJ
Date published: 2024-10-03

Que dilución es recomendable usar para WB en suero humano

Asked by: Mari30
Thank you for your question. Galectin-3 Antibody (M3/38): sc-23938 is recommended for detection of galectin-3 of mouse, rat and human origin by Western Blotting at a starting dilution 1:200 (general dilution range 1:100-1:1000)
Answered by: Tech Support Europe
Date published: 2024-09-17

I would like to stain galectin-3 on renal sections by IHC. Which demasking conditions do you recommend for this antibody (EDTA, citrate, etc.) ? Which dilution as well ?

Asked by: pierrelo
Thank you for your question. Our recommended method is as follows: Heat treatment: Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2024-01-04

Is the antibody raised against the C or N-terminus of the protein?

Asked by: Maria93
Thank you for your question.The antibody is raised against the N-terminus.
Answered by: BlakeJ
Date published: 2023-08-30

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: cjMara
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-22

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Thank you for your question. We have not tested this antibody in canine samples, however this antibody clone has been published on dog samples (for example, PMID: 20705359). Please contact Technical Service by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-01-12
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Rated 5 out of 5 by from Good for WB and IPThe antibody presents good staining for WB and also works for immunoprecipitation. Tested in human and rat cells.
Date published: 2024-08-23
Rated 5 out of 5 by from ExcellentIt works for IF nicely. Very bright signal was detected.
Date published: 2024-01-17
Rated 5 out of 5 by from Molto buono!Ho testato questo anticorpo per immunoistochimica su polmoni di topo in paraffina perché stavo cercando un marcatore alternativo per l'identificazione dei macrofagi. La colorazione risulta essere chiara e pulita e sembra ben identificare i macrofagi alveolari.
Date published: 2021-04-13
Rated 5 out of 5 by from Great FeaturesI got the sample antibody a month ago and I probed the lysates for the galectin 3 expression. The bands were good even at 1:400 dilution. The original recommended dilution in the SDS was 1:100
Date published: 2019-09-16
Rated 5 out of 5 by from galectin 3 antibody (sample) recomendableAB sample was tested for IF (1/50) and gave perfect results! Highly recommended since it gave very nice pictures. Will order it.
Date published: 2018-04-11
Rated 5 out of 5 by from Wow !! Superb product !Really strong western blot data; also outstanding IHC !!
Date published: 2017-01-31
Rated 5 out of 5 by from Excellent rat monoclonalThere are several very good galectin-3 clones to choose from, however this galectin-3 (M3/38) clone provides an option of a rat host with excellent data in Western blot and IHC(P). Plus, this clone is featured in several recent publications!
Date published: 2017-01-30
Rated 4 out of 5 by from Publication in IF with mouse leg musclesPublication in IF with mouse leg muscles. -SCBT Publication Review
Date published: 2015-04-21
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galectin-3 抗体 (M3/38) is rated 4.6 out of 5 by 12.
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