Date published: 2025-9-7

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FTα 抗体 (D-5): sc-374262

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说明书
  • FT alpha 抗体 D-5 是小鼠单克隆 IgG3 (kappa轻链),FTα抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的FTα的C-terminus的氨基酸349-377之间
  • 推荐用于 mouse, rat 和 human 来源的FTα WB, IP, IF 和 ELISA检测
  • 关于如何获取FTα (D-5): sc-374262免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRP是FTα Antibody (D-5) 适用于 WB 应用。 的首选辅助检测试剂,该试剂现与FTα Antibody (D-5) 搭配使用(请参阅下面的订购信息)。

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    FTα抗体(D-5)是一种IgG3 κ小鼠单克隆FTα抗体(也被称作FTα抗体),该抗体可通过蛋白质印迹、免疫沉淀、免疫荧光和酶联免疫吸附测定检测小鼠、大鼠和人的FTα蛋白。FTα抗体(D-5)可作为非共轭抗FTα抗体使用。哺乳动物蛋白法尼基转移酶是包含两个非同一性的α和β亚基的异二聚体蛋白,它们将法尼基残基连接到几种蛋白质(包括核纤层蛋白和p21Ras蛋白)的COOH末端第四位半胱氨酸上。天然底物含有Cys-A-A-Xaa识别序列,其中A残基为脂肪族,Xaa代表甲硫氨酸、丝氨酸、谷氨酰胺或半胱氨酸。纯化的法尼基转移酶是αβ异二聚体。β亚基与肽底物结合,而α亚基则参与与底物法尼基焦磷酸形成稳定的复合物。α亚基与第二种异戊烯基转移酶即香叶基香叶基转移酶共用,后者将含有20个碳的香叶基香叶基连接到Ras相关蛋白上,这些蛋白终止于Cys-A-A-Xaa识别位点,其中Xaa为亮氨酸。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    FTα 抗体 (D-5) 参考文献:

    1. 原子分辨率的蛋白法尼基转移酶反应路径。  |  Long, SB., et al. 2002. Nature. 419: 645-50. PMID: 12374986
    2. 克隆和表达编码大鼠 p21ras 蛋白法尼基转移酶 alpha 亚基的 cDNA。  |  Chen, WJ., et al. 1991. Proc Natl Acad Sci U S A. 88: 11368-72. PMID: 1763049
    3. 蛋白质异戊烯化的序列依赖性。  |  Moores, SL., et al. 1991. J Biol Chem. 266: 14603-10. PMID: 1860864
    4. 大鼠脑 p21ras 蛋白法尼基转移酶识别肽的序列要求。  |  Reiss, Y., et al. 1991. Proc Natl Acad Sci U S A. 88: 732-6. PMID: 1992464
    5. 蛋白法尼基转移酶和香叶基转移酶共享一个共同的α亚基。  |  Seabra, MC., et al. 1991. Cell. 65: 429-34. PMID: 2018975
    6. p21H-ras 法尼基转移酶的非相同亚基。肽结合和焦磷酸法尼基载体功能。  |  Reiss, Y., et al. 1991. J Biol Chem. 266: 10672-7. PMID: 2037606
    7. Cys-AAX 四肽对纯化的 p21ras 法呢酰:蛋白转移酶的抑制作用。  |  Reiss, Y., et al. 1990. Cell. 62: 81-8. PMID: 2194674
    8. Ha-ras 癌基因蛋白的翻译后修饰:第三类蛋白羧基甲基转移酶的证据。  |  Clarke, S., et al. 1988. Proc Natl Acad Sci U S A. 85: 4643-7. PMID: 3290900
    9. 人 CAAX 法尼基转移酶两个亚基的 cDNA 克隆以及 FNTA 和 FNTB 基因座及相关序列的染色体图谱。  |  Andres, DA., et al. 1993. Genomics. 18: 105-12. PMID: 8276393

    订购信息

    产品名称产品编号规格价格数量收藏夹

    FTα 抗体 (D-5)

    sc-374262
    200 µg/ml
    $316.00

    FTα (D-5): m-IgG3 BP-HRP 套装

    sc-550577
    200 µg Ab; 40 µg BP
    $354.00

    FTα (D-5) 中和勝肽

    sc-374262 P
    100 µg/0.5 ml
    $68.00

    Good morning, I ran this immunoblot and ended up with three bands, between approximately 38 kDa and 55 kDa. What bands are correct/ what do each of the bands represent? Two were at the low end, and one was at the high end, if this helps. Thanks, Robyn

    Asked by: rxb345
    Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
    Answered by: Technical Service
    Date published: 2022-11-02

    Est ce que cet anticorps reconnait la CAAX box (KDGKKKKKKSKTKCVIM) ? Merci

    Asked by: Christine75
    Merci pour votre question. Veuillez contacter notre Service Technique pour toute information complémentaire sur cet anticorps. Thank you for your question. Please contact our Technical Service
    Answered by: SCBT Heidelberg Support
    Date published: 2018-12-22

    What application is the blocking peptide sc-374262 P appropriate for?

    Asked by: AbPolly
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-28
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    Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmicExcellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-01-05
    Rated 5 out of 5 by from Produced positive Western Blot data of FTProduced positive Western Blot data of FT expression in NIH/3T3 whole cell lysate. -SCBT QC
    Date published: 2013-10-13
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    FTα 抗体 (D-5) is rated 5.0 out of 5 by 2.
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